LOCUS       sequence-245428-        2166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic 
            terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2166)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T17m
FEATURES             Location/Qualifiers
     source          1..2166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..26
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(30..73)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    76..97
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    185..205
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(211..228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(382..970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(462..481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1141..1932,1933..2001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1764..1783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2002..2106)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
       61 taaggatgat ttctggaatt cgcggccgct tctagagact agtgggtctc aaggttctcg
      121 gtaccaaatt ccagaaaaga gacgctttcg agcgtctttt ttcgttttgg tccgcttaga
      181 gacctactag tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      361 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
      421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1261 cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa
     1321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2101 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2161 attaac
//
