LOCUS       sequence-24554-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mRFP-RBD(R59A)-mRFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          100..232
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     215..234
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        277..295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      315..324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(451..469)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gnggtagttt atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg
       61 aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
      121 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
      181 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
      241 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
      301 ctcgagaatt cgtcgccacc atggcctcct ccgaggacgt catcaaggag ttcatgcgct
      361 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      421 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      481 tgcccttcgc ctgggacatc ctgtcccctc agttccagta cggctccaag gcctacgtga
      541 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      601 agcgcgtgat gaacttcgag gacggcggcg tgggngaccg tgacccnnnn ncttccnggn
      661 nnngnngcga gttcatctac aaaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      721 cccgtaatgc agaaagaaga ccatggggct gggagggcct ccacccgagc gggatgtacc
      781 cccgagggac ggcgccctgg
//
