LOCUS       sequence-245648-        7944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP7.12-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2722..2739
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2791..2846
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2845..2864)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5082..5100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5168..5272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5273..5341,5342..6133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5491..5510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6304..6892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6793..6812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7046..7063
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7180..7201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7216..7221,7222..7239,7240..7246)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7254..7270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7259..7281
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7278..7294
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7278..7294
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7565..7944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaagagaa agatcacaac agtgggccag
      841 ggcggcgact acaacgagaa cgtggtcaac atcctgagcc agaaactggg caacgtgtac
      901 aacgccgacc ccagcaacgc caacgaggcc gtgaacaaga agtacaagct gatcaagaaa
      961 gagatggaag aactgcagca gtacgagaaa gagaacgccg aagaggactt ccagcagaac
     1021 ttcatgaccg agacagacga gacaagcggc aagaagaaaa ccatctttgg cgtggacgag
     1081 gacgacctgg acagctacga cgaggaattc ttcggccaga gcaagaagtt catcaagggc
     1141 gaggccagcg gcgacgacgt gcacagcgga aatagcctgg aagccgagga acccaaagag
     1201 gtggccagca gccctcctca tggcgatctg gccggaacag gcctgacacc tggcagacag
     1261 agcgagatcg agcagctgaa gggcgagaga gtggaaaccg gccctgacgt gaagatcagc
     1321 agcacccatg tgcggagcga cggctctcct gagagcgccc ctaaagagag cctgcagcag
     1381 cccaacaccg ccgacctgga agcagaaaga cgggctggcg tgaccacccc ttctgacggc
     1441 cagcagagaa cagtggctgc cggccctaca cctagcagcc cacctcccaa tccccaggac
     1501 cctagcaaga ccagcgaccc caagggcagc gagagccaga gaaacgtgca ggaaaccagc
     1561 gaggtccaga gagagaacct ggaaagcagc accggcaacc tggaacctgg cgtgccatct
     1621 gccgtggccg aggatacaaa gcagcctccc gagcagatca ccgaggccac agcccctgtg
     1681 gtcaagtaca tggacagcct gtacgacgag atcatcacca agagcggcca gaacgagaag
     1741 gccgagaaca ccaagaagca cagcaactac gccaacctga agaaaaagta caacttcccc
     1801 atgaacggca aagagtacaa catggtcaag cagctgttcg gcgagtgctt taagaaagag
     1861 gacaccgcca acgtcgccga gaatgccgcc tgtcaggtgg acgtgctggc caacgtgctg
     1921 caggacaaag agttcagcga gcagttcgac aacttcatgc acggcctgta cagcttcgcc
     1981 aagcggcaca cctacctgag cgacgccaga ctgaaggacg agaacgccat caacggcttt
     2041 ctgaagaacg ccctgagcct gctgaacaca ggcgcgccgt cgacctccat cacggcctat
     2101 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     2161 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     2221 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     2281 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     2341 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     2401 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     2461 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     2521 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     2581 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc
     2641 ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct
     2701 cctctggccc ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa
     2761 ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     2821 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     2881 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
     2941 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     3001 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     3061 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     3121 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     3181 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     3241 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     3301 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     3361 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     3421 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     3481 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     3541 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     3601 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     3661 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     3721 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     3781 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     3841 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     3901 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     3961 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     4021 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     4081 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     4141 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     4201 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     4261 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     4321 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     4381 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     4441 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     4501 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4561 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     4621 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4681 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     4741 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4801 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4861 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4921 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4981 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     5041 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     5101 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5161 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5221 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5281 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5341 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5401 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5461 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5521 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5581 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5641 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5701 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5761 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     5821 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5881 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5941 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6001 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6061 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6121 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6181 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6841 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6901 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6961 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7021 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7081 ratacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7141 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7201 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7261 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     7321 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     7381 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     7441 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     7501 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     7561 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7621 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7681 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7741 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7801 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     7861 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     7921 gtattagtca tcgctattac catg
//
