LOCUS       sequence-245692-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial 
            cell culture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Figard L, Zheng L, Biel N, Xue Z, Seede H, Coleman S, Golding I, 
            Sokac AM
  TITLE     Cofilin-Mediated Actin Stress Response Is Maladaptive in 
            Heat-Stressed Embryos.
  JOURNAL   Cell Rep. 2019 Mar 26;26(13):3493-3501.e4. doi: 
            10.1016/j.celrep.2019.02.092.
  PUBMED    30917306
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.02"; journalName: "Cell Rep"; date: 
            "2019-03-26- 26"; volume: "26"; issue: "13"; pages: "3493-3501.e4"
COMMENT     Sequence Label: pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(537..551)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(570..587)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             600..622
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    637..661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(768..787)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcctgcactc gctgtgctag tagacgagtc catgtgctgg cgttcaattt cgcagcagcg
       61 gtttctttac cacactcgag ttattggcgg tcggtggccc ggagtttctc ctcgacggct
      121 tcccgggagg cttcggaaag atcagtggct tgtatatact tttggacgcc cacgagcgac
      181 ttcttgagag catcgaagga gctggagtac aacatcttct tcttgacctt ggcagtatcg
      241 ggacaccacg acataaggaa cagcttttgc ttctttgaac tctcagaggt gccttggcac
      301 tggtgcatgt actcgaaatc gaacagtccg taccggcact ctccaggtcc gcatttctgg
      361 atatcttcta gaaactggtc gtactcggcg ttgcgatctg ccacagtctc cacatcaatc
      421 tgcttttcat cgcgaatgta aaagatgaca tagcgatgct ttttgtcctt ctttatttcc
      481 tcgtatgtag tcttgcagac atcagacaca gttacaccag aagcgggatc cggactcttg
      541 tcgtcgtcat cattcgaacc ggtacccacg tgatggtggt ggtgatgtgc catggtatat
      601 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc
      661 ccctatagtg agtcgtatta atttcgcggg atcgagatcg atctcgatcc tctacgccgg
      721 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      781 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      841 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      901 attccttgcg gcggcggtgc tcaacgggcc tcaacctact actgggctgc ttcctaatgc
      961 aggagtcgca taaggagagc gtcgagatcc cggacatcat cgaatggcgc aaaaccttcg
     1021 cgtatgggca tgatagcgcc ctgaagagag tcattcaggt ggtgaatgtg cacagttacg
     1081 taacgatgtc gcagagttat gcgggtgttc tcctatatcc agaaaccgtt tttctg
//
