LOCUS       sequence-245751-        7758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP7.8-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2536..2553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2605..2660
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2659..2678)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4896..4914)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4982..5086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5087..5155,5156..5947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5305..5324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6118..6706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6607..6626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6860..6877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6994..7015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7030..7035,7036..7053,7054..7060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7068..7084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7073..7095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7092..7108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7092..7108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7379..7758
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaaagtga ccgccaagaa caagcagaag
      841 aactacgcca aagagggctt cctgaacctg ctgaacaaga agctggaaaa cctgaacaac
      901 gccgtgtccg gcgaggtgtc cgccaacttc aacaaggaca tcgagctgct gaagaaagag
      961 atcgaggaac tgcagaagta cgagaaggcc tacggcgccg aggatttcga cgagaacctg
     1021 gaagatgaga gcggcgacgg cctgggcaag aagaaaaaga tcctgggcgt ggacgaggac
     1081 gagctggaca actacgacga ggaattcttc ggccagagca agaccaccat gaagggcgag
     1141 gactaccagg aagataccga cgctcaggac gacaccgacg agcagggcga gacagatgcc
     1201 caggatgaca gcagcggcga ggatcaccac atggtgcctg gcgagatcgc cgagggcaat
     1261 agccctctgg aaggcaccaa cgacaagggc gctgcccaga agtctggcga gttcccaccc
     1321 aaagagacaa gcaacggcaa ggtgtccctg gatggcgccg accctcacaa cgaggccagc
     1381 aatggacatg atgccccacc tggcgaggtg ccaccttacg gcgtggaaag caccgatcct
     1441 gaggtggccc ctggaaagca gaaggcccct gagcagggct tcaacaccgc caacgccgag
     1501 gtgcagtacc tggacaacct gtacgacgac gccatcagag agacaaacaa gaacaaccag
     1561 gtgcacaacc ctcagcacca caccaactac gataccctga acaaaaacta cgacttcccc
     1621 atgaccagca aagaatacga catggtcaag atgctgttcg gcgactgctt caagatgggc
     1681 aacgacgaga atagcaacac cgtgtgcctg atcgacctgt tcaagaagat tctcgaggac
     1741 aagatcttta tgaagcagtt cgagaacttc atgcacagca tctacagctt cgccaagcgg
     1801 cacaactacc tgggccacga gaagatgaag aacgaggaac tctacaaaga gctgttcaac
     1861 agcgccctga agctgctcaa cacaggcgcg ccgtcgacct ccatcacggc ctataagagt
     1921 gagggggagt cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga
     1981 gagttgagat ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta
     2041 aagaaccaaa aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa
     2101 acgctcccac tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac
     2161 ctgaccctga ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa
     2221 gtgactcagc ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag
     2281 atgagactga tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg
     2341 attcaagtgc aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag
     2401 gtcaagatgg actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac
     2461 cacatcctgg acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg
     2521 gcccctctgg gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg
     2581 atcccccgac ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     2641 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga
     2701 tccctcggag atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta
     2761 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     2821 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     2881 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     2941 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     3001 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     3061 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     3121 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     3181 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     3241 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     3301 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     3361 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     3421 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     3481 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     3541 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     3601 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     3661 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     3721 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     3781 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     3841 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     3901 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     3961 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     4021 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     4081 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     4141 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     4201 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     4261 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     4321 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4381 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     4441 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     4501 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     4561 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4621 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4681 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4741 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4801 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag
     4861 ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     4921 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4981 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5221 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5281 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     5341 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5401 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5461 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5521 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5581 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     5641 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5701 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5761 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5821 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5881 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5941 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6001 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6061 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6241 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6481 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6841 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6901 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6961 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7021 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7081 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     7141 ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg
     7201 caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc
     7261 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     7321 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
     7381 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     7441 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7501 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7561 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     7621 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     7681 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     7741 gtcatcgcta ttaccatg
//
