LOCUS       sequence-245755-        8193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP3.10-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2971..2988
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3040..3095
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3094..3113)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5331..5349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5417..5521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5522..5590,5591..6382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5740..5759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6553..7141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7042..7061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7295..7312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7429..7450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7465..7470,7471..7488,7489..7495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7503..7519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7508..7530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7527..7543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7527..7543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7814..8193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaagatgc ccagagaggt ggacaacacc
      841 aagaacatcg gcaacatgaa gcggagcctg ccccacaact tctacgtgaa gaatatcgcc
      901 ctgcaggacg aggaagagaa gcggaccctc caggtggacc ctcccagcca ggaagaggcc
      961 agaagcgtcc agacacccca gagcgagctg aaccccgatc agaaagaggc ccggacagag
     1021 aacggccagg ccggactgga agccctgcct tctgagggcc agaatctgca gggccggacc
     1081 ctgtctacca acgccgccgt gaacggcatc atcaacgagc ccaacgtgga cacccaggaa
     1141 gaacaccacg gcagcaccca gaacctgaag gacaaagagc agcagcccga cgccgagaac
     1201 agccggtcca tcggaaatat cgtgggagcc caggataccc aggaactgag ccaggaaaaa
     1261 gagagcaaca ccgccatcag cagaggcgag cacagcgaga aagagatcac caaagagaca
     1321 gccaccggca agaccagcaa cggccacacc gagacagccg agatctctgc cggaaaggcc
     1381 ctgaagccca gcgtgcagcc taatgccctg cctgccgata aggccgagag aagcctggaa
     1441 accgccaccg gcctgaacaa ggatcagcct gccagcccta gcggcgagga acccctgatc
     1501 aagcctgaag tgggcaccac cctgaacagc aagagccccg aggaagatcg gggcgaaaaa
     1561 gaaaagggcc ccgagcccca cagagccagc gagtctgaac agctgccagc cgagaagtcc
     1621 accgaccaga aactgagcgt gtccgagggc gacctgccca gcacactgga tgtgaaggcc
     1681 ccaagcggcc cacagaacga ggacctgagc acacaggaac cccagtccgt cgacaacgag
     1741 cagggcaaag aagaggacga agaggaacgc aagggcgtcg aggaacatca ggaagagagc
     1801 cccagcagcc tgtccgagaa agtggacaac cagaacatcc tggaacccgt gaacctgccc
     1861 cagggcaaga cccacaacac cgtggatgag gatgagaaag tgatcgagga aaatgaggaa
     1921 aagcagaaag aagaagatga ggaagatatc atcaaggccg aagaggtgca gaacatcgtc
     1981 aaagagattc tgcaggaagg ggaggaagag ggaaaagagg agggcgagga agaggaagag
     2041 gacgaggaac aggccggcga gtacaactcc cacggcaagc aggataagac cgagggcgtg
     2101 aacaacgagc tgctgaaaga gaaagaggaa agcacccctc ctcccaacga cgccaccctg
     2161 tacaagtact tcagcatgga atacgaggac gacaatgagg ccaagaagtt cgccgacgag
     2221 ctgatcaaca ccctggtcac cagcgtgggc ggcgacacca gcaccgtgaa caccctgaag
     2281 gatctggaaa aggatatgcg gcagttcttc ctcaacattg gcgcgccgtc gacctccatc
     2341 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     2401 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     2461 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     2521 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     2581 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     2641 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     2701 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     2761 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     2821 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     2881 tccggcagcc tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg
     2941 aacctgcctc ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca
     3001 cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     3061 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3121 atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg
     3181 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     3241 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     3301 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     3361 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     3421 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     3481 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     3541 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     3601 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     3661 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     3721 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     3781 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     3841 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     3901 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     3961 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     4021 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     4081 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     4141 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     4201 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     4261 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     4321 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     4381 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     4441 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     4501 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     4561 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     4621 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     4681 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     4741 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4801 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     4861 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4921 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     4981 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     5041 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     5101 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5161 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5221 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     5281 tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat
     5341 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5521 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5581 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5641 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5701 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5761 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5821 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5881 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5941 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6001 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6061 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6121 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6181 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6241 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6301 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6361 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6421 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6481 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6661 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7201 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7261 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7321 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7381 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7441 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7501 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     7561 ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     7621 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     7681 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     7741 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     7801 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     7861 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     7921 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     7981 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8041 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     8101 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     8161 tacatctacg tattagtcat cgctattacc atg
//
