LOCUS       sequence-245898-        2135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for Bujard RBS (strong RBS from 
            https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2135)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U17m
FEATURES             Location/Qualifiers
     source          1..2135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..76
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    82..102
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             120..131
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    159..180
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      183..226
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(230..248)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        316..420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(421..489,490..1281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(639..658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1452..2040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1941..1960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
       61 cgagtcagtg agcgaggaag cctgcagcgg ccgctactag taggtctctc attggtacct
      121 ttctcctctt taatgaattc agtatgagac ccactagtct ctagaagcgg ccgcgaattc
      181 cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg cctcgtgata
      241 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      541 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      601 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      661 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      721 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      781 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      841 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      901 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      961 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1021 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1081 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcg
     1141 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1201 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1261 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1321 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1381 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1441 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1501 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1561 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1621 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1681 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1741 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1801 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1861 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1921 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1981 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctgtggaaa
     2041 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2101 ttctttcctg cgttatcccc tgattctgtg gataa
//
