LOCUS       sequence-245901-        2149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for T500 terminator Engineered 
            terminator derived from bacteriophage 83; see DOI: 
            10.1126/science.284.5414.611)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2149)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T1m
FEATURES             Location/Qualifiers
     source          1..2149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..54
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    60..80
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    151..172
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      175..218
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(222..240)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        308..412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(413..481,482..1273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(631..650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1444..2032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1933..1952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
       61 tgcagcggcc gctactagta ggtctctaag ctctcaagga cagaaaagcc cgcctttcgg
      121 cgggctttga gtaccttgag acccactagt ctctagaagc ggccgcgaat tccagaaatc
      181 atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt
      241 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      421 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      481 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      541 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      601 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      661 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      721 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      781 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      841 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      901 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      961 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1021 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1081 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt cgcgcggtat
     1141 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1201 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1261 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1321 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga aaaacgccag
     2041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2101 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtga
//
