LOCUS       sequence-245970-       11241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-XTEN-TdT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, 
            Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
  TITLE     Lineage tracing and analog recording in mammalian cells by 
            single-site DNA writing.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 
            10.1038/s41589-021-00769-8. Epub 2021 Mar 22.
  PUBMED    33753928
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00769-8"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "739-747"
COMMENT     Sequence Label: pTBL210
FEATURES             Location/Qualifiers
     source          1..11241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             917..5020
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             917..5020
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             917..5020
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5033..5053
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6676..6693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6682..6906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6952..7380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7039..7058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7249..7270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7389..7409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7394..7723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7574..7709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7636..7655
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8914..9035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8951..8970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9005..9024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9084..9100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9084..9100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9097..9119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9108..9124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9132..9138,9139..9156,9157..9162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9177..9198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9315..9332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9486..10074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9566..9585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10245..11036,11037..11105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10868..10887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11106..11210)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cctgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccggtc gccaccatgg acaagaagta ctccattggg ctcgatatcg gcacaaacag
      961 cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct
     1021 gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc tgttcgactc
     1081 cggggagacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag
     1141 aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga
     1201 ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg
     1261 ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat
     1321 atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta
     1381 tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa
     1441 cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct
     1501 tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag
     1561 gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg agaagaagaa
     1621 cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact ttaaatctaa
     1681 cttcgacctg gccgaagatg ccaagcttca actgagcaaa gacacctacg atgatgatct
     1741 cgacaatctg ctggcccaga tcggcgacca gtacgcagac ctttttttgg cggcaaagaa
     1801 cctgtcagac gccattctgc tgagtgatat tctgcgagtg aacacggaga tcaccaaagc
     1861 tccgctgagc gctagtatga tcaagcgcta tgatgagcac caccaagact tgactttgct
     1921 gaaggccctt gtcagacagc aactgcctga gaagtacaag gaaattttct tcgatcagtc
     1981 taaaaatggc tacgccggat acattgacgg cggagcaagc caggaggaat tttacaaatt
     2041 tattaagccc atcttggaaa aaatggacgg caccgaggag ctgctggtaa agcttaacag
     2101 agaagatctg ttgcgcaaac agcgcacttt cgacaatgga agcatccccc accagattca
     2161 cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat ttctacccct ttttgaaaga
     2221 taacagggaa aagattgaga aaatcctcac atttcggata ccctactatg taggccccct
     2281 cgcccgggga aattccagat tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc
     2341 ctggaacttc gaggaagtcg tggataaggg ggcctctgcc cagtccttca tcgaaaggat
     2401 gactaacttt gataaaaatc tgcctaacga aaaggtgctt cctaaacact ctctgctgta
     2461 cgagtacttc acagtttata acgagctcac caaggtcaaa tacgtcacag aagggatgag
     2521 aaagccagca ttcctgtctg gagagcagaa gaaagctatc gtggacctcc tcttcaagac
     2581 gaaccggaaa gttaccgtga aacagctcaa agaagactat ttcaaaaaga ttgaatgttt
     2641 cgactctgtt gaaatcagcg gagtggagga tcgcttcaac gcatccctgg gaacgtatca
     2701 cgatctcctg aaaatcatta aagacaagga cttcctggac aatgaggaga acgaggacat
     2761 tcttgaggac attgtcctca cccttacgtt gtttgaagat agggagatga ttgaagaacg
     2821 cttgaaaact tacgctcatc tcttcgacga caaagtcatg aaacagctca agaggcgccg
     2881 atatacagga tgggggcggc tgtcaagaaa actgatcaat gggatccgag acaagcagag
     2941 tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca
     3001 gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg
     3061 ccagggggac agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa
     3121 gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa
     3181 gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa
     3241 gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat
     3301 ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta
     3361 cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga
     3421 ctacgacgtg gatcatatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa
     3481 agtgttgaca agatccgata aaaatagagg gaagagtgat aacgtcccct cagaagaagt
     3541 tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg
     3601 gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg
     3661 cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct
     3721 cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt
     3781 tattactctg aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt
     3841 gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac
     3901 tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt
     3961 gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa
     4021 gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg
     4081 agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga
     4141 caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt
     4201 taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa
     4261 cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga
     4321 ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa
     4381 aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga
     4441 aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat
     4501 cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc
     4561 tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt
     4621 cttgtatctg gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa
     4681 gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga
     4741 attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa
     4801 taagcacagg gataagccca tcagggagca ggcagaaaac attatccact tgtttactct
     4861 gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag acagaaagcg
     4921 gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct
     4981 ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg accccaagaa
     5041 gaagaggaag gtgagcggca gcgagactcc cgggacctca gagtccgcca cacccgaaag
     5101 tatggatcca ccacgagcgt cccacttgag ccctcggaag aagagacccc ggcagacggg
     5161 tgccttgatg gcctcctctc ctcaagacat caaatttcaa gatttggtcg tcttcatttt
     5221 ggagaagaaa atgggaacca cccgcagagc gttcctcatg gagctggccc gcaggaaagg
     5281 gttcagggtt gaaaatgagc tcagtgattc tgtcacccac attgtagcag agaacaactc
     5341 gggttcggat gttctggagt ggcttcaagc acagaaagta caagtcagct cacaaccaga
     5401 gctcctcgat gtctcctggc tgatcgaatg cataggagca gggaaaccgg tggaaatgac
     5461 aggaaaacac cagcttgttg tgagaagaga ctattcagat agcaccaacc caggcccccc
     5521 gaagactcca ccaattgctg tacaaaagat ctcccagtat gcgtgtcaga gaagaaccac
     5581 tttaaacaac tgtaaccaga tattcacgga tgcctttgat atactggctg aaaactgtga
     5641 gtttagagaa aatgaagact cctgtgtgac atttatgaga gcagcttctg tattgaaatc
     5701 tctgccattc acaatcatca gtatgaagga cacagaagga attccctgcc tggggtccaa
     5761 ggtgaagggt atcatagagg agattattga agatggagaa agttctgaag ttaaagctgt
     5821 gttaaatgat gaacgatatc aatccttcaa actctttact tctgtatttg gagtggggct
     5881 gaagacttct gagaagtggt tcaggatggg tttcagaact ctgagtaaag taaggtcgga
     5941 caaaagcctg aaatttacac gaatgcagaa agcaggattt ctgtattatg aagaccttgt
     6001 cagctgtgtg accagggcag aagcagaggc cgtcagtgtg ctggttaaag aggctgtctg
     6061 ggcatttctt ccggatgctt tcgtcaccat gacaggaggg ttccggaggg gtaagaagat
     6121 ggggcatgat gtagattttt taattaccag cccaggatca acagaggatg aagagcaact
     6181 tttacagaaa gtgatgaact tatgggaaaa gaagggatta cttttatatt atgaccttgt
     6241 ggagtcaaca tttgaaaagc tcaggttgcc tagcaggaag gttgatgctt tggatcattt
     6301 tcaaaagtgc tttctgattt tcaaattgcc tcgtcaaaga gtggacagtg accagtccag
     6361 ctggcaggaa ggaaagacct ggaaggccat ccgtgtggat ttagttctgt gcccctacga
     6421 gcgtcgtgcc tttgccctgt tgggatggac tggctcccgg cagtttgaga gagacctccg
     6481 gcgctatgcc acacatgagc ggaagatgat tctggataac catgctttat atgacaagac
     6541 caagaggata ttcctcaaag cagaaagtga agaagaaatt tttgcgcatc tgggattgga
     6601 ttatattgaa ccgtgggaaa gaaatgccta ggcggccgct cgagtctaga gggcccgttt
     6661 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7261 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7321 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7381 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7741 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgcagctctc
     7801 ggagggcgaa gaatatcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     7861 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     7921 ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta
     7981 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     8041 cgctgttctg caaccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     8101 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     8161 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     8221 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     8281 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     8341 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     8401 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     8461 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     8521 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     8581 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     8641 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     8701 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gcgggactct ggggttcgaa
     8761 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     8821 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8881 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9061 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9121 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9181 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9241 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9301 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10261 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10321 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10381 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10441 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10501 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10561 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10621 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10681 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10741 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10801 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10861 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10921 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10981 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11041 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11101 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11221 cgaaaagtgc caccttacgt c
//
