LOCUS       sequence-24599-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Pluta K, Luce MJ, Bao L, Agha-Mohammadi S, Reiser J
  TITLE     Tight control of transgene expression by lentivirus vectors 
            containing second-generation tetracycline-responsive promoters.
  JOURNAL   J Gene Med. 2005 Jun . 7(6):803-17.
  PUBMED    15655804
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2005 Jun . 7(6):803-17."
COMMENT     Sequence Label: pNL-EGFP/TREPittdU3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(85..87,88..90,91..801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDXLYK"
     primer_bind     complement(130..151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(391..410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     738..759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggttnnnnn nngnngcgtg ancatcacgc ntnttttgac ctccatagaa gacaccggga
       61 ccgatccagc ctggatcccc gcggatggtg agcaagggcg aggagctgtt caccggggtg
      121 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      181 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      241 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      301 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      361 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      421 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      481 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      541 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      601 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      661 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      721 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      781 ggcatggacg anctgtacaa gtcggactca gatccgactc nagactaaaa aactgancat
      841 ccaat
//
