LOCUS       sequence-246032-        2347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of p85-GFP in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT p85-GFP
FEATURES             Location/Qualifiers
     source          1..2347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..48
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             1591..2304
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1591..2301
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1618..1646)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2241..2261
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2324..2341)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 caccatcatc acgaagatca agttgaccca agacttattg acggaaaagg aggaggaggc
       61 cggcccatgc ggccacaggc gcccggatcg ctggtcgatc ccaacgagga cgagctacgc
      121 atggccccat ggtactgggg ccggatatcg agggaggaag ccaagagcat cctgcacgga
      181 aagccggacg gcagcttttt ggtgcgggac gccctctcga tgaagggcga gtacactctg
      241 accctgatga aggacggatg cgagaagcta ataaagatat gccacatgga tcgcaagtac
      301 ggctttatcg agacggacct gttcaattcg gtggtggaga tgatcaacta ctacaaggag
      361 aactctctga gcatgtacaa caagacgctg gacataacac tgagtaatcc gattgtgcga
      421 gcccgcgagg atgaggagtc gcagccgcac ggggatcttt gtctgctgag caacgaattc
      481 atcaggacct gccagctgct tcagaatctt gagcaaaatc tcgagaataa gcgcaactca
      541 ttcaatgcga ttcgagagga gctgcaggag aagaagctgc accagagcgt attcgggaat
      601 acagagaaga tattccgcaa tcaaatcaag ctcaatgaat ccttcatgaa ggcgccagcg
      661 gatgccccgt ccacggaggc aggaggagct ggagatggag ctaacgcagc cgcatccgca
      721 gcggccaatg ccaacgctcg ccggagtctg caggagcaca agcagacact cctcaatctg
      781 ctggatgctc ttcaggccaa gggtcaagtc cttaaccact acatggagaa caagaagaag
      841 gaggagctac tgctcgagcg acagatcaac gccctgaagc cggaattaca gatactgcag
      901 ttgcgcaagg acaagtacat tgagcgccta aaaggattca acctcaagga cgacgatctg
      961 aagatgatat tgcagatggg cttcgacaag tggcagcaac tttatgagac agtctccaac
     1021 cagccgcaca gcaacgaagc tctctggttg ctaaaggatg ccaagagaag gaatgcggag
     1081 gagatgctta agggggcgcc atcgggcacc ttcctgattc gagcgagaga tgcgggtcac
     1141 tatgctctgt ccattgcctg caagaacatc gtgcagcact gccttattta cgagacgagc
     1201 accggctttg gatttgctgc cccttacaat atatatgcca cgctgaagag tctggtggag
     1261 cactatgcca acaattcgct ggaggagcat aacgacacgc tgaccacgac attgcgctgg
     1321 cctgtcttgt actggaagaa caacccgctg caagttcaga tgatccaatt gcaggaggaa
     1381 atggatctgg agtacgagca ggcagccacg ttgaggccgc cgccaatgat gggcagcagt
     1441 gcgccgattc ccacgagtcg gtcccgtgaa catgacgtcg tagatggaac tggaagcctc
     1501 gaggcggagg cggctccggc ctccatttcg ccctccaact tcagcacatc gcaggggcgc
     1561 gccggcggcc gctcgagtct agagggcccc agcaaaggag aagaactttt cactggagtt
     1621 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
     1681 gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
     1741 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
     1801 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
     1861 tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa
     1921 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa
     1981 gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac
     2041 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt
     2101 gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2161 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc
     2221 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
     2281 ggcatggatg agctctacaa ataggtttaa acccgctgat cagcctcgac tgtgccttct
     2341 aagatcc
//
