LOCUS       sequence-24609-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: CMV-Brainbow-1.0 L
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(82..115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(267..285)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     535..554
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgagctca taacttcgta taggatactt
       61 tatacgaagt tataactcga gataacttcg tatagcatac attatacgaa gttataccgg
      121 cagatctgcc accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
      181 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      241 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      301 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
      361 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
      421 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
      481 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
      541 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
      601 cgtgctgaa
//
