LOCUS       sequence-246124-        2915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 0 vector of PlaMiNGo toolkit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pTEI125
FEATURES             Location/Qualifiers
     source          1..2915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             325..966
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQTVLSKDPNEK"
     primer_bind     complement(1029..1047)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1115..1219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1441..2232
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2403..2915,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2892..2911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatgtcc aaaggagaag aactgtttac cggtgttgtg
      361 ccaattttgg ttgaactcga tggtgatgtc aacggacata agttctcagt gagaggcgaa
      421 ggagaaggtg acgccaccat tggaaaattg actcttaaat tcatctgtac tactggtaaa
      481 cttcctgtac catggccgac tctcgtaaca acgcttacgt acggagttca gtgcttttcg
      541 agatacccag accatatgaa aagacatgac ttttttaagt cggctatgcc tgaaggttac
      601 gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat ataaaactag agcagttgtt
      661 aaatttgaag gagatacttt ggttaaccgc attgaactga aaggaacaga ttttaaagaa
      721 gatggtaata ttcttggaca caaactcgaa tacaatttta atagtcataa cgtatacatc
      781 actgctgata agcaaaagaa cggaattaaa gcgaatttca cagtacgcca taatgtagaa
      841 gatggcagtg ttcaacttgc cgaccattac caacaaaaca cccctattgg agacggtccg
      901 gtacttcttc ctgataatca ctacctctca tatcaaacag tcctgagcaa agatccaaat
      961 gaaaaaggaa caggtggcgg cggaagttag ctttgagacc acgaagtggc tcttcagtgg
     1021 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1081 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1201 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     1261 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta
     1321 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     1381 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     1441 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     1501 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     1561 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     1621 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1681 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1741 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     1801 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     1861 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     1921 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     1981 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     2041 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     2101 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     2161 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     2221 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2281 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2341 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2521 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2761 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2881 gagcttccag ggggaaacgc ctggtatctt tatag
//
