LOCUS       sequence-246199-       15376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15376)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX005
FEATURES             Location/Qualifiers
     source          1..15376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5490..5514)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5543..5632
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5666..5918
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6347..6599)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7067..8092)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7067..8092)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8102..8446)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8588..8605)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8588..8604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8597..8619)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8744..8763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8954..8975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9222..9246
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10842..11990)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12289..13083)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13275..14042
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14735..15366
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggctat
     4621 gaaagaaaag ctaaatggcg gtttgaaagg aagaaatgtt aaaactaacg gagtgaaaga
     4681 ggttcactac agaggagtaa gaaagaggcc atggggacgt tacgctgctg aaatccgtga
     4741 tcccagcaag aagtgcaggg tttggttggg caccttcgac acggcggaag aggcggcaaa
     4801 ggcttatgat accgccgcca gagagtttcg tggacccaaa gccaagtcga acttcccctt
     4861 gccgtcggag aatcagtgtc ccagtaacag cagtggacca acagagtcgt ccagtggaga
     4921 aaccggtgtt cacgctcctc acgcaccctc tgagctcgac ctcacgcgcc cccttggaac
     4981 cctcgctgta ggcggtgacc atggtggttg taaatcagaa gttggattgg tgagaaatgg
     5041 gattccgatt ttccatcatc aacctgttat gggagttctg actagtgacg acagtggcgt
     5101 tgcagagtcc tctagtggag aaaccggcgt tcacgcgcct cttgagctcg acctcacgcg
     5161 ccatctcggc gccgttggtg agggtggcaa ccatgtccga aatgggtttc ccgtttttta
     5221 tcagcaaccg acagtggcgg ttctgcctaa tggcctgcca attttgttgt tcgacagtac
     5281 ttttgtgcca accggagtat ggaaccggca aaagccgtac aggtttgaac cggtgggggt
     5341 tgagtttaac ggcgttagtg gtagggtagt tcatagcgac gcgagctcat ccgctgcggt
     5401 gaaggagaat cattacggag ggggcggcgt aggtagaaga gaactaaatc ttgatctaaa
     5461 ccttgcccca ccaatggaag ctcccgggga cccagctttc ttgtacaaag tggttgataa
     5521 cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg gctatcccta
     5581 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     5641 cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     5701 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     5761 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     5821 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     5881 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt catcaacgca
     5941 agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga aaggacttgc
     6001 tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt gggttttcga
     6061 tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg cagtcagcag
     6121 ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt ggcgcaaacc
     6181 agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt cggcatgagc
     6241 agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct tttgttagtc
     6301 agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc tagtaacata
     6361 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     6421 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     6481 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     6541 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     6601 gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg tgggtagcac
     6661 caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat ttgccagttg
     6721 gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg aggggtattt
     6781 cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat tcttcagaaa
     6841 aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt gacggtgcta
     6901 ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat tcctgcaaga
     6961 gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg tctatttgct
     7021 cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat tcctttgccc
     7081 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     7141 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     7201 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     7261 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     7321 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     7381 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     7441 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     7501 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     7561 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     7621 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     7681 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     7741 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     7801 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     7861 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     7921 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     7981 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     8041 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcggggt
     8101 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     8161 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     8221 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     8281 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     8341 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     8401 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     8461 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     8521 gacggatctc taggacgcgt cgacgcgtcg acgcgtcgac gcgtcgacgc gtcctagaag
     8581 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8641 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8701 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc
     8761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     8881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     9001 tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa caggattttc
     9061 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     9121 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa
     9181 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     9241 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     9301 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     9361 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     9421 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     9481 atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct
     9541 taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata
     9601 aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac
     9661 tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc
     9721 ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac
     9781 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc
     9841 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg
     9901 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt
     9961 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat
    10021 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt
    10081 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg
    10141 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc
    10201 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa
    10261 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat
    10321 ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc
    10381 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc
    10441 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta
    10501 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc
    10561 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat
    10621 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat
    10681 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga
    10741 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg
    10801 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
    10861 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
    10921 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
    10981 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
    11041 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
    11101 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
    11161 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
    11221 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
    11281 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
    11341 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
    11401 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
    11461 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
    11521 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
    11581 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
    11641 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
    11701 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
    11761 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
    11821 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
    11881 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
    11941 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
    12001 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
    12061 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
    12121 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc
    12181 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag
    12241 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc
    12301 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    12361 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    12421 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    12481 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    12541 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    12601 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    12661 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    12721 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    12781 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    12841 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    12901 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    12961 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    13021 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    13081 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    13141 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    13201 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    13261 aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
    13321 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
    13381 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa
    13441 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt
    13501 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc
    13561 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
    13621 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
    13681 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
    13741 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
    13801 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
    13861 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
    13921 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
    13981 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
    14041 cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc
    14101 catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc
    14161 cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata tattatctta
    14221 tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct
    14281 tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt
    14341 tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc
    14401 cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg
    14461 gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca
    14521 ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca
    14581 cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc
    14641 cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg
    14701 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg
    14761 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
    14821 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
    14881 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
    14941 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
    15001 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
    15061 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
    15121 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
    15181 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
    15241 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct
    15301 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg
    15361 agcgtcggaa aggcgc
//
