LOCUS       sequence-24624-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Targeted gene inactivation in zebrafish using engineered zinc-finger
            nucleases.
  JOURNAL   Nat Biotechnol. 2008 Jun . 26(6):695-701.
  PUBMED    18500337
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Jun . 26(6):695-701."
COMMENT     Sequence Label: 1352wUV2 F1bbs kan GCGTGGNNN
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             215..484
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             494..517
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atnctacgcg caatgcaacc tcccaattca agtgcggaca acaacccttt gcaacagcaa
       61 tcatcacaaa ataccgtacc aaacgtcctc aaccaaatta accaaatctt ttctccagag
      121 gagcaacgca gcttattaca agaagccatc gaaacctgca agaattttga aaaaacacaa
      181 ttgtaagtct cggctccaag aaggagatat acccatggca cgcgtaactg ttcaggacgc
      241 tgtagagaaa attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca
      301 gatgcaggta ggcggaaagg acccgctcgt accggaagaa aacgataaaa ccactgtaat
      361 cgcgctgcgc gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg
      421 ccaggaacag caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg
      481 tcgtgcggcc gcggactaca aggatgacga cgacaagttc cggaccggtt ccaagacacc
      541 cccccatggt acccgcccat atgcttgccc tgtcgagtcc tgtggtcttc taagtgagga
      601 tccaaacncg gncgggncan ccgagcgttc tgaaatgaat ccagatggag ttctgaggtc
      661 attactggat cttatcaaca ggagtccaag cngagctctc gaaccccaga gtcccgctca
      721 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gattcgggag ggncaanccg
      781 taangcacga gnagcggt
//
