LOCUS       sequence-24625-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Targeted gene inactivation in zebrafish using engineered zinc-finger
            nucleases.
  JOURNAL   Nat Biotechnol. 2008 Jun . 26(6):695-701.
  PUBMED    18500337
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Jun . 26(6):695-701."
COMMENT     Sequence Label: pCS2-HA-GAAZFP-FokI-RR
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..47
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             48..137
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gtnttttgcg gactgccacc atggctccaa agaagaagcg taaggtatac ccatacgatg
       61 ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcg tatccatatg
      121 acgttccaga ttacgctgct catggtaccc gcccatatgc ttgccctgtc gagtcctgtg
      181 accgccgctt cagccgtagc gacgcgctca cccgccatat ccggattcac acaggccaga
      241 agcccttcca gtgtcgcatc tgcatgagga acttcagctg tgcgcatcac ctcacccgcc
      301 acatccgcac ccacacaggc gagaagcctt ttgcatgcga catctgcggc aggaagttcg
      361 cgcagttgtc taacctcacc cgccatacca aaatccatac aggtggatcc caactagtca
      421 aaagtgaact ggaggagaag aaatctgaac ttcgtcataa attgaaatat gtgcctcatg
      481 aatatattga attaattgaa attgccagaa attccactca ggatagaatt cttgaaatga
      541 aggtaatgga attttttatg aaagtttatg gatatagagg taaacatttg ggtggatcaa
      601 ggaaaccgga cgnagntntn tatacgggcg gatctcctat tgataacggt gtgatcgtgg
      661 atactaannt aangaggagg ttataatctg ccaattggcc agccggtnag tgccaangtt
      721 tgcnaaaaaa tcaaccnnaa acaactttca ccctangang gggaagtctt cctctttgta
      781 cgaatta
//
