LOCUS       sequence-246265-       15251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15251)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX011
FEATURES             Location/Qualifiers
     source          1..15251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5397..5421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5450..5539
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5573..5825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6254..6506)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6974..7999)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6974..7999)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8009..8353)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8463..8480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8463..8479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8472..8494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8619..8638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8829..8850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9097..9121
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10717..11865)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12164..12958)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13150..13917
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14610..15241
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcgcc
     4621 gaagcaactg ggtggcgcgg tggcggttac ggcggcggtt ggagaagtga atggaatttc
     4681 aaaagaggta cattataggg gcgtaaggaa gaggccatgg gggaggtatg cggcggagat
     4741 aagagatccg gggaaaaaga gccgggtttg gctcgggact tttgatacgg cggaggaagc
     4801 agcgaaagcc tacgatgcgg cggcgagaga attccgcggt gcaaaggcga aaactaattt
     4861 tcctccgtcg gaagaagaga acctcaaaat cgcccaaaac aatttaggcg ttaagatgta
     4921 taagaacgtt aatgataata acaatcgtag tccgagtcag agcagtaccg ttgagtcatc
     4981 tagccgtgac gggttatctc ctgccgttat ggttgactca tcgccgttag atctcagcct
     5041 cggcgtgaaa ttcccgttcc agaacaatca gttccgcact tccccgattc ccggcgggtt
     5101 ttccggctgc gggtttaccg gcgcaactcc tgcgatgaat catcacctgt attatctgga
     5161 ggctctggca cgtgcgggag taataaatct ggaaaaacaa cagcaaaggc agacggttga
     5221 tttccttggc ggtaacggtg gacatggcgg cagtagcggc ggtagcggaa gtgggaccca
     5281 gagtgaatct gattcatcat cggtgattga gtttatgcgt aacgatgtta agccccctac
     5341 cagaacaagt cttaatctgg acctaaattt tcctccaccg gagaacgcgc ccggggaccc
     5401 agctttcttg tacaaagtgg ttgataacag cgggttaatt aacatctttt acccatacga
     5461 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     5521 tgacgttcca gattacgctg ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc
     5581 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     5641 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     5701 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     5761 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     5821 agatcgggaa ttagcttcat caacgcaaga catgcgcacg accgtctgac aggagaggaa
     5881 tttccgacga gcacagaaag gacttgctct tggacgtagg cctatttctc aggcacatgt
     5941 atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc gccgccaact gggagatgag
     6001 gaggctttct tggggggcag tcagcagttc atttcacaag acagaggaac ttgtaaggag
     6061 atgcactgat ttatcttggc gcaaaccagc aggacgaatt agtgggaata gcccgcgaat
     6121 atctaagtta tgcctgtcgg catgagcaga aacttccaat tcgaaacagt ttggagaggt
     6181 tgtttttggg catacctttt gttagtcagc ctctcgattg ctcatcgtca ttacacagta
     6241 ccgaagtttg atcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     6301 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     6361 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     6421 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     6481 ctttattgcc aaatgtttga acgatctgct tcgacgcact ccttctttac tccaccatct
     6541 cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac
     6601 caatcacgct ggatgatttg ccagttggat taatcttgcc tttccccgca tgaataatat
     6661 tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat agctgcaatt gccgcgacat
     6721 cctccaacga gcataattct tcagaaaaat agcgatgttc catgttgtca gggcatgcat
     6781 gatgcacgtt atgaggtgac ggtgctaggc agtattccct caaagtttca tagtcagtat
     6841 catattcatc attgcattcc tgcaagagag aattgagacg caatccacac gctgcggcaa
     6901 ccttccggcg ttcgtggtct atttgctctt ggacgttgca aacgtaagtg ttggatcccg
     6961 gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     7021 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     7081 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     7141 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     7201 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     7261 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     7321 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     7381 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     7441 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     7501 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     7561 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     7621 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt
     7681 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     7741 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     7801 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     7861 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     7921 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     7981 tgagttcagg ctttttcata tcggggtcgt cctctccaaa tgaaatgaac ttccttatat
     8041 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     8101 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     8161 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     8221 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     8281 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     8341 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     8401 gacgagagtg tcgtgctcca ccatgttgac ggatctctag gacgcgtcct agaagctaat
     8461 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8521 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8581 cgcccttccc aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc
     8641 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8701 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     8761 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8821 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     8881 ttttgattta taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg
     8941 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     9001 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt
     9061 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     9121 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     9181 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     9241 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     9301 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     9361 atatcatctc cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc
     9421 gtgacaggct gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc
     9481 gctgaggaag ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc
     9541 tatccattgc tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat
     9601 gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg
     9661 taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca
     9721 ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa
     9781 acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg
     9841 tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa
     9901 ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca
     9961 ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg
    10021 ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg
    10081 tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg
    10141 aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc
    10201 ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc
    10261 ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct
    10321 tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc
    10381 cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt
    10441 gctcgtaaac ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc
    10501 ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca
    10561 attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg
    10621 agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc
    10681 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
    10741 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
    10801 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
    10861 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
    10921 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
    10981 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
    11041 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
    11101 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
    11161 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
    11221 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
    11281 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
    11341 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
    11401 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
    11461 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
    11521 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
    11581 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga
    11641 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
    11701 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
    11761 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
    11821 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
    11881 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
    11941 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
    12001 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
    12061 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
    12121 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca
    12181 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
    12241 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
    12301 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
    12361 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
    12421 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
    12481 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
    12541 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
    12601 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    12661 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    12721 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    12781 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    12841 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    12901 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    12961 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    13021 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    13081 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    13141 acaattatgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
    13201 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
    13261 taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg
    13321 gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat
    13381 ggccatgaat ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac
    13441 gattttacgt cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc
    13501 agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg
    13561 cgccagcgct ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta
    13621 ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac
    13681 gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg
    13741 cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag
    13801 cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac
    13861 aaagtcatcg ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa
    13921 ttatgataga atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc
    13981 aagtttttat gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc
    14041 cccctgaaat ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt
    14101 attgtcaata tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta
    14161 gctttttaaa tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata
    14221 cctcggtata atcttaccta tcacctcaaa tggttcgctg ggtttatcgc acccccgaac
    14281 acgagcacgg cacccgcgac cactatgcca agaatgccca aggtaaaaat tgccggcccc
    14341 gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc acccaggccg
    14401 ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca
    14461 atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc gatcccggca
    14521 atggcaagga ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc
    14581 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc
    14641 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt
    14701 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg
    14761 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg
    14821 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc
    14881 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat
    14941 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct
    15001 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc
    15061 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag
    15121 gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc
    15181 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt
    15241 cggaaaggcg c
//
