LOCUS       sequence-24630-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Nie M, Htun H
  TITLE     Different modes and potencies of translational repression by 
            sequence-specific RNA-protein interaction at the 5'-UTR.
  JOURNAL   Nucleic Acids Res. 2006  . 34(19):5528-40.
  PUBMED    17023487
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2006  . 34(19):5528-40."
COMMENT     Sequence Label: pMN cFKT
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             330..356
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    419..540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     484..503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cacggtttcn ggcctatatt ttgtttactt taaaaggaga tatagccatg ggaaaccaaa
       61 agcatncntg tgattttgtc gtcattaaag cggtcgagga cggcgtgaac gtcatcggtt
      121 taacgagagg aacggatacg aaatttcacc attccgaaaa actggacaaa ggcgaagtga
      181 ttatcgctca gtttactgag catacctctg ccatcaaagt gagaggcaac gccctcatcc
      241 agaccgctta cggagaaatg aaaagcgaga agaagggcgc ctttggcgcg ctcgaattca
      301 cgcgtggtac ctctagagtc gaccagctgt atccatacga cgtgccagat tacgctggac
      361 accatcacca ccatcacgcc tgactgacta actaagcggc cgcttcgagc agacatgata
      421 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      541 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
      601 taaagcaagt aaaacctcta caaatgtggt aaattcgata aggatcctcg gngctaacca
      661 agcccgnaaa ggaagctgng ttgnctgctg ccaccgctga agcatnacta cataccccct
      721 nggggcctct aacgggntct gaggggtttt tgctgaaggg aggactatat agatccggct
      781 ggcgtatanc gaaaagccgc cccgacgcct cccacagttg ncacctgant gggatggccg
      841 cctgngngnn nttatagcgc gggngg
//
