LOCUS       sequence-24634-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe H-Ras V12 S35
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(20..52,53..55,56..589)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPSIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(608..628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggnnnngatt cnctcgacca tgacggaata taagctggtg gtggtgggcg ccgtcggtgt
       61 gggcaagagt gcgctgacca tccagctgat ccagaaccat tttgtggacg aatacgaccc
      121 cagtatagag gattcctacc ggaagcaggt ggtcattgat ggggagacgt gcctgttgga
      181 catcctggat accgccggcc aggaggagta cagcgccatg cgggaccagt acatgcgcac
      241 cggggagggc ttcctgtgtg tgtttgccat caacaacacc aagtcttttg aggacatcca
      301 ccagtacagg gagcagatca aacgggtgaa ggactcggat gacgtgccca tggtgctggt
      361 ggggaacaag tgtgacctgg ctgcacgcac tgtggaatct cggcaggctc aggacctcgc
      421 ccgaagctac ggcatcccct acatcgagac ctcggccaag acccggcagg gagtggagga
      481 tgccttctac acgttggtgc gtgagatccg gcagcacaag ctgcggaagc tgaaccctcc
      541 tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgag gatctagtcg
      601 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      661 agtatgnnnn nngnnnntnn nnnnnagtca ngcaaccagg ngnnggaaag tccccagggc
      721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcnncaac catagtcccg
      781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgccccatt cctccgcccc
//
