LOCUS       sequence-246470-       15439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15439)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX029
FEATURES             Location/Qualifiers
     source          1..15439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4745..4765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(5553..5577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5606..5695
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5729..5981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6410..6662)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7130..8155)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7130..8155)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8165..8509)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8651..8668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8651..8667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8660..8682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8807..8826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9017..9038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9285..9309
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10905..12053)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12352..13146)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13338..14105
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14798..15429
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggatgg
     4621 aaacggtatg aatatacgaa attggggttt tttcgagcca gctgcaacag tcctgaaggg
     4681 ccatttaggc ctgcaactca tggcctctat ggatgagaag cccctttttg gcaacattcg
     4741 cgaccatcac catcaccatc accacaacca ccaaacccac cagcctcacc atcctacagt
     4801 tatggagtca actaatggtg gtgcgtttca tcaccaccgg gtttgtggga tcttggagac
     4861 ccccatgccc actgagtaca tgagggattc ttgggtcaat cataaagact atagagaaaa
     4921 gtatctcagt gtgctatccg gaaaccacca ctatcttacg ggttatggtt tttttcctga
     4981 gacgtcttct gctcagtcca tgcagattca cgagcagcca aacttggtga aagtggaacc
     5041 tgatcctcag ctggaagagg tttgtctaga aagggatatt ggtgggcttg caaagaagag
     5101 gggagctggt aaatcacaac tgctgaagtc ccctaaaagt aagagggcaa agaaagctac
     5161 tagggtccct aaagtcgagt ctaccccatc tgttccacgg gcaagagctc ccagaaaaag
     5221 tgcagaagtt gtcattaatg gtattactat ggatatttcg gtaattccca taccaatttg
     5281 ctcatgcact ggggctgctc agcagtgtta taggtggggc tgtggtggtt ggcaatcggc
     5341 ttgctgtacc actaatttat cttcatatcc cttgcctatg aatgccaaaa ggcgtggttc
     5401 aaggatagca ggacggaaga tgagtttggg agcgtttaca aaggtactag aaaaacttgc
     5461 ttcggaagga tataactttt ctaacgcaat tgatctgaag ccttactggg caaagcatgg
     5521 tacaaacaag tttgtcacta tcagacccgg ggacccagct ttcttgtaca aagtggttga
     5581 taacagcggg ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     5641 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     5701 gtgcagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     5761 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5821 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     5881 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     5941 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgatcaaact tcggtactgt
     6001 gtaatgacga tgagcaatcg agaggctgac taacaaaagg tatgcccaaa aacaacctct
     6061 ccaaactgtt tcgaattgga agtttctgct catgccgaca ggcataactt agatattcgc
     6121 gggctattcc cactaattcg tcctgctggt ttgcgccaag ataaatcagt gcatctcctt
     6181 acaagttcct ctgtcttgtg aaatgaactg ctgactgccc cccaagaaag cctcctcatc
     6241 tcccagttgg cggcggctga tacaccatcg aaaacccacg tccgaacact tgatacatgt
     6301 gcctgagaaa taggcctacg tccaagagca agtcctttct gtgctcgtcg gaaattcctc
     6361 tcctgtcaga cggtcgtgcg catgtcttgc gttgatgaag ctaattcccg atctagtaac
     6421 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     6481 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     6541 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     6601 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     6661 tctgcttcga cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag
     6721 caccaaaacg aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag
     6781 ttggattaat cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta
     6841 tttcgatttt ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag
     6901 aaaaatagcg atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg
     6961 ctaggcagta ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca
     7021 agagagaatt gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt
     7081 gctcttggac gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg
     7141 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     7201 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     7261 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     7321 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     7381 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     7441 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     7501 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     7561 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     7621 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     7681 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     7741 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     7801 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     7861 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     7921 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     7981 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     8041 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     8101 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg
     8161 ggtcgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     8221 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     8281 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     8341 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     8401 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     8461 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     8521 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     8581 gttgacggat ctctaggacg cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag
     8641 aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8701 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8761 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     8821 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8881 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     8941 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9001 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9061 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     9121 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     9181 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     9241 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     9301 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     9361 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     9421 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     9481 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     9541 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
     9601 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
     9661 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
     9721 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
     9781 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
     9841 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
     9901 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
     9961 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
    10021 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
    10081 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
    10141 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
    10201 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
    10261 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
    10321 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
    10381 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
    10441 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
    10501 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
    10561 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
    10621 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    10681 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    10741 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    10801 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    10861 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    10921 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    10981 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    11041 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    11101 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    11161 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    11221 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    11281 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    11341 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    11401 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    11461 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    11521 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    11581 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    11641 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    11701 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    11761 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    11821 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    11881 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    11941 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    12001 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    12061 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    12121 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    12181 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    12241 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    12301 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    12361 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    12421 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    12481 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    12541 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    12601 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    12661 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    12721 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    12781 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    12841 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    12901 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    12961 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    13021 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    13081 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    13141 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    13201 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    13261 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    13321 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    13381 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
    13441 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    13501 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    13561 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    13621 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    13681 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    13741 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    13801 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    13861 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    13921 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    13981 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    14041 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    14101 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    14161 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    14221 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    14281 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    14341 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    14401 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    14461 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    14521 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    14581 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    14641 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    14701 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    14761 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    14821 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    14881 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    14941 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    15001 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    15061 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    15121 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    15181 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    15241 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    15301 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    15361 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    15421 gtgagcgtcg gaaaggcgc
//
