LOCUS       sequence-246475-        6870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent expression of SomArchon under CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, 
            Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa 
            E, Sabatini BL, Fu Z, Boyden ES, Han X
  TITLE     Population imaging of neural activity in awake behaving mice.
  JOURNAL   Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. 
            Epub 2019 Oct 9.
  PUBMED    31597963
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
            volume: "574"; issue: "7778"; pages: "413-417"
COMMENT     Sequence Label: pAAV-CAG-FLEX-SomArchon
FEATURES             Location/Qualifiers
     source          1..6870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   56..185
                     /label=AAV2 ITR
     polyA_signal    347..468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(384..403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     438..457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    511..1099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             617..628
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1026..1046
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1141..1174
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1245..1278
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      1295..1304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2168..2170,2171..2881)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2168..2170,2171..2881)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2210..2231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2471..2490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2818..2839
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3149..3182)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3245..3278)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(3313..3332)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3394..3416)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3695..3972)
                     /label=chicken beta-actin promoter
     repeat_region   4283..4412
                     /label=AAV2 ITR
     rep_origin      4484..4939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4571..4590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4781..4802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4965..5069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5070..5138,5139..5930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5288..5307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6101..6689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6590..6609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatccttatc gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      301 tctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc
      481 gaagcggccg cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
      541 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      601 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      661 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
      721 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      781 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      841 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      901 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      961 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1021 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1081 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttcgaacctc ttcgagggac
     1141 ataacttcgt ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga
     1201 gggtcgatgg tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag
     1261 tatcctatac gaagttatac tagaattcgg taccgccacc atggtttcta tcgcactgca
     1321 ggctggttat gacctcctcg gagatggtag acctgaaagt ctgtggctcg gaattggtac
     1381 actgctgatg ctgatcggaa ccttctactt cctggttaga gcgtggggag agactgacaa
     1441 ggatgcacgg gagtactacg cagtgacaat cctggtcagc ggtattgcca gtgcagctta
     1501 tctgtcaatg ttctttggta ttggcctcac agaggtgcca gtaggtggag agatgctgaa
     1561 catctactac gcaagatacg cacagtggct gttcactaca ccactgttgc tgctgcacct
     1621 ggcactcctg gccaaagttg atagagtgac catcggcact ctcgttggcg tcgatgctct
     1681 gatgattgtc acaggcctga taggaacact gtcacatact gccattgcca gatactcttg
     1741 gtggctgttc tcaactatct gtatgatcgt cgttctgtat gtcctggcca cttctctgcg
     1801 gtcagccgct aaggagagag gcccagaggt tgcctcaacc ttcaacatcc tcaccgcact
     1861 ggttctggtc ctgtggactg cttaccctat catctggatt atcggcaccg agggtgccgg
     1921 agttgtgggc ctgggaatcg aaactttgct gttcatggtg ctggacgtca ccgccaaggt
     1981 gggcttcggt tttatcctgc tcaggtcaag agccattctg caagatacag aagcacctga
     2041 accttcagct ggagctgata ccggtgccgc cgaccgcccg gtagtagcag tgagcaaggc
     2101 ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat
     2161 caacgtggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2221 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2281 ctacggcaag ctgaccctga agttcatttg caccaccggc aagctgcccg tgccctggcc
     2341 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2401 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2461 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2521 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2581 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2641 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2701 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2761 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2821 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2881 gggtgggagc ggaggtaccc agagccagcc tatcctgaac accaaggaga tggcccctca
     2941 gagcaagcct cctgaggagc tggagatgag cagcatgcct agccctgtgg cccctctgcc
     3001 tgccaggacc gagggcgtga tcgacatgag gagcatgagc agcatcgaca gcttcatcag
     3061 ctgcgccacc gacttccctg aggccaccag gttctacaag ttctgctacg agaacgaggt
     3121 gtaatgtaca agtaagggga tccctagtat aacttcgtat agcatacatt atacgaagtt
     3181 atgggtcgat ggtgatgctt ggcaattcgg gtcgatggtg aagcattggt cttcctattt
     3241 tgatataact tcgtatagga tactttatac gaagttatgg ggatccaatt ctttgccaaa
     3301 atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc
     3361 atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc
     3421 cctgccccgt cccccccgaa ggcagccgtc cccccgcgga cagccccgag ccgctccccc
     3481 cgcacaaagg gccctcccgg agcccctcaa ggctttcacg cagccacaga aaagaaacaa
     3541 gccgtcatta aaccaagcgc taattacagc ccggaggaga agggccgtcc cgcccgctca
     3601 cctgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg gcggcggagc
     3661 ggggcacggg gcgaaggcag cgcgcagcga ctcccgcccg ccgcgcgctt cgctttttat
     3721 agggccgccg ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg ccgctctgat
     3781 tggctgccgc cgcacctcyc cgccycsccc csccccsccc cycsccccsc cccsccccsc
     3841 cyggcgcgcg cccccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa
     3901 ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg
     3961 ggctcacctc gaccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4021 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4081 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4141 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     4201 acgtcattat tgacgtcaat ggtactcttc ctagagcatg gctacgtaga taagtagcat
     4261 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg
     4321 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4381 cgggcggcct cagtgagcga gcgagcgcgc agctggcgta atagcgaaga ggcccgcacc
     4441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4681 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4801 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4921 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5281 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6241 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6481 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6781 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6841 gcagcgagtc agtgagcgag gaagcggaag
//
