LOCUS       sequence-246477-       13809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13809)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 13809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX033
FEATURES             Location/Qualifiers
     source          1..13809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2911..2935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3923..3947)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3976..4065
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4099..4351
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(4780..5032)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5500..6525)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5500..6525)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6535..6879)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7021..7038)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7021..7037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7030..7052)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7177..7196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7387..7408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7655..7679
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9275..10423)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10722..11516)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11708..12475
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      13168..13799
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag aacacggggg acaagtttgt acaaaaaagc aggctccacc
     2941 actagtatga cggctgccgg tgatagaatg acgtccggcg gatcatcggg gcggttgccg
     3001 acgtggaagg agagggaaaa caataagagg agagagagga gaagaagagc tatcgcagcc
     3061 aaaatattta ctgggctacg agctcaagga aacttcaaac tccccaagca ctgtgataat
     3121 aacgaggtct taaaagctct ctgtctagaa gctggttggg tcgttgaaga tgatggcacc
     3181 acttatcgca agggaaacag gcctccaccg atggaaaatg gtggtgcctc aatgaataac
     3241 agcacatgct catcgattca gcctagccca atgtcgtcct cttaccctag tcatgtacct
     3301 tcttaccatg ccagcccaac atcatcctca ttccctagcc cctcccgttg tgacggaaat
     3361 ccaccatcat acatcctccc ctttctccat aacctagctt acattccctc ttctctccca
     3421 cctcttcgtg tatctaacag tgcccctgtt accccacctc tttcttctcc tacccgaaac
     3481 tcaaacccca aacctgtctg ggaatcactc tccagtgttc cattgcattc tttccaccac
     3541 ccgctttttg ctgcttctgc accatcaagt cccactcgcc gccaatactc caagcctgct
     3601 acaattccag aatgtgatga gtctgatgtc gcctcagttg tgtctgcacg ctggggcagc
     3661 ttccagacgg gggcaccttc agctgctcca acttcgccta cttttaacct tgtaaagcct
     3721 attcctcagc agaatattct cttagatgcc ttaagtggcc gtggggcttt tggctgggct
     3781 gaagcagctc acaagggaca tggctctgaa tttaattttg aaagctgcaa agtgatggca
     3841 tgggaaggtg agcgaataca tgaagttgct gttgatgatt tagagctcac tcttgggagt
     3901 ggaaagccac gtgctcccgg ggacccagct ttcttgtaca aagtggttga taacagcggg
     3961 ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
     4021 ccggactatg caggatccta tccatatgac gttccagatt acgctgctca gtgcagcgct
     4081 tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     4141 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     4201 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     4261 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     4321 tcgcgcgcgg tgtcatctat gttactagat cgggaattag cttcatcaac gcaagacatg
     4381 cgcacgaccg tctgacagga gaggaatttc cgacgagcac agaaaggact tgctcttgga
     4441 cgtaggccta tttctcaggc acatgtatca agtgttcgga cgtgggtttt cgatggtgta
     4501 tcagccgccg ccaactggga gatgaggagg ctttcttggg gggcagtcag cagttcattt
     4561 cacaagacag aggaacttgt aaggagatgc actgatttat cttggcgcaa accagcagga
     4621 cgaattagtg ggaatagccc gcgaatatct aagttatgcc tgtcggcatg agcagaaact
     4681 tccaattcga aacagtttgg agaggttgtt tttgggcata ccttttgtta gtcagcctct
     4741 cgattgctca tcgtcattac acagtaccga agtttgatcg atctagtaac atagatgaca
     4801 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     4861 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     4921 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     4981 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttcga
     5041 cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag caccaaaacg
     5101 aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag ttggattaat
     5161 cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta tttcgatttt
     5221 ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag aaaaatagcg
     5281 atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg ctaggcagta
     5341 ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca agagagaatt
     5401 gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt gctcttggac
     5461 gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg ccctcggacg
     5521 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     5581 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     5641 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     5701 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     5761 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     5821 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     5881 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     5941 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     6001 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     6061 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     6121 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     6181 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     6241 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     6301 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     6361 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     6421 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     6481 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc
     6541 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     6601 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     6661 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     6721 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     6781 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     6841 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     6901 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttgacggat
     6961 ctctaggacg cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag aagctaattc
     7021 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7081 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7141 cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct
     7201 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7261 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     7321 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7381 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     7441 ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct
     7501 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     7561 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc
     7621 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     7681 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     7741 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     7801 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     7861 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaaat
     7921 atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc gcttaaccgt
     7981 gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg ataaagccgc
     8041 tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc aactccccta
     8101 tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc ggcctgatgc
     8161 atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga aacaactgta
     8221 ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg
     8281 ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg cggatcaaac
     8341 actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg
     8401 agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc
     8461 gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc
     8521 aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa tcgtatcggg
     8581 ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc gcctaccgtc
     8641 gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca gaatagcgaa
     8701 atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg
     8761 acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc ggcgacccct
     8821 cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg agcgtccttg
     8881 gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg
     8941 aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc
     9001 tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg
     9061 aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac aattgtcaat
     9121 tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag cgatactgag
     9181 cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg
     9241 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     9301 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     9361 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     9421 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     9481 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     9541 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     9601 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     9661 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     9721 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     9781 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     9841 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     9901 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     9961 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
    10021 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc
    10081 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
    10141 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
    10201 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
    10261 cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg
    10321 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
    10381 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
    10441 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct
    10501 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
    10561 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
    10621 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
    10681 gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt
    10741 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    10801 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    10861 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10921 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10981 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    11041 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    11101 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    11161 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    11221 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    11281 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    11341 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    11401 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    11461 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    11521 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    11581 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    11641 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
    11701 aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
    11761 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
    11821 acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc
    11881 aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
    11941 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga
    12001 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
    12061 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
    12121 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
    12181 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
    12241 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
    12301 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
    12361 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
    12421 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttacccaatt
    12481 atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa
    12541 gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc
    12601 cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc ttatcagtat
    12661 tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg tcttggtagc
    12721 tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc
    12781 tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac ccccgaacac
    12841 gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc
    12901 catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc
    12961 gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat
    13021 gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat
    13081 ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
    13141 cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac
    13201 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
    13261 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
    13321 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
    13381 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
    13441 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    13501 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    13561 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    13621 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    13681 atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
    13741 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg
    13801 gaaaggcgc
//
