LOCUS       sequence-246589-       16757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16757)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX056
FEATURES             Location/Qualifiers
     source          1..16757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6871..6895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6924..7013
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7047..7299
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7728..7980)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8448..9473)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8448..9473)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9483..9827)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9969..9986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9969..9985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9978..10000)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10125..10144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10335..10356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10603..10627
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12223..13371)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13670..14464)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14656..15423
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16116..16747
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat gagttcggaa aattggagtt tgacggcgaa gctttgcgat
     5881 tcgtgcaaaa cgacgtcggc gacggtgttc tgccgggcag attctgcttt tttatgcata
     5941 ggatgcgact gtaaaatcca cgcggcgaat aagctagcgt cacgtcacgc gcgggtttgg
     6001 gtttgcgagg tgtgcgagca ggcgccggcg agcgtcacgt gcaaggcgga cgccgccgct
     6061 ctctgcgtca cgtgcgaccg cgatatccac tctgcgaatc cattagcgcg tcgtcacgag
     6121 cgtttccccg ttgttccctt ctacgacacc gccgtcgcaa aatctcacgg cggcgttaat
     6181 gatgatgaga agtactttga tagtcatgag aataatcctc aaccggagga agaggcggag
     6241 gcggcgtcgt ggatacttcc gacgccgaaa gaagggactg ataatcagta taaatcagcg
     6301 gattatttgt ttaatgatat ggattcgtat ttggatatgg atactatgtc gtgtgatcaa
     6361 aaaccgaata atattcttca gctgtaccag cagtactgct ctgatggagt ggtgccagtg
     6421 cagaacaaga acgagagtag ccatttgcaa ggtccagttg ttaatggatt tccaacgtac
     6481 gaaatggact tcattggatc taaaccatac ttgtacaact tcaactctca gtccattagc
     6541 caaagtgttt cttcatcatc tatggaagtg ggagttgtgc cggagcacag tgcaacggcg
     6601 gatgtatcaa acagcttagt gatgaactca tcgggggaca ccgtaccgaa cccagtctct
     6661 ggtttagaca gagaagcaag ggtattgagg tacagagaga aaaggaagaa cagaaagttc
     6721 gagaagacta ttcggtatgc ttctagaaag gcttacgcag agacccgacc cagaatcaaa
     6781 ggaagattcg caaagcgtac tgagattgaa gtcgactccc ttctcgccgc cgccgatgcg
     6841 tcttatggcg ttgttccatc gttttcgagg acccagcttt cttgtacaaa gtggttgata
     6901 acagcgggtt aattaacatc ttttacccat acgatgttcc tgactatgcg ggctatccct
     6961 atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt
     7021 gcagcgctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     7081 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     7141 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     7201 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     7261 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc
     7321 aagacatgcg cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg
     7381 ctcttggacg taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg
     7441 atggtgtatc agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca
     7501 gttcatttca caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac
     7561 cagcaggacg aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag
     7621 cagaaacttc caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt
     7681 cagcctctcg attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat
     7741 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     7801 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     7861 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     7921 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     7981 tgcttcgacg cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca
     8041 ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt
     8101 ggattaatct tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt
     8161 tcgattttgg caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa
     8221 aaatagcgat gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct
     8281 aggcagtatt ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag
     8341 agagaattga gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc
     8401 tcttggacgt tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc
     8461 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     8521 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     8581 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     8641 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
     8701 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     8761 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     8821 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     8881 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     8941 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     9001 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
     9061 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
     9121 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     9181 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca
     9241 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     9301 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     9361 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     9421 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg
     9481 tcgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     9541 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     9601 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     9661 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     9721 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     9781 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     9841 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     9901 tgacggatct ctaggacgcg tcgacgcgtc gacgcgtcga cgcgtcgacg cgtcctagaa
     9961 gctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10021 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10081 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt
    10141 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    10201 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
    10261 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    10321 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
    10381 ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt
    10441 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
    10501 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa
    10561 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
    10621 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
    10681 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
    10741 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
    10801 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
    10861 gatcaaatat catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc
    10921 ttaaccgtga caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat
    10981 aaagccgctg aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa
    11041 ctcccctatc cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg
    11101 cctgatgcat ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa
    11161 caactgtagg ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc
    11221 cgatcaggcc gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg
    11281 gatcaaacac taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg
    11341 taaaggtgag cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca
    11401 tggaaaccgc ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg
    11461 tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc
    11521 gtatcgggct acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc
    11581 ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga
    11641 atagcgaaat attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa
    11701 tctgtcggac gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg
    11761 cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag
    11821 cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt
    11881 aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct
    11941 cctcgtgctc gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga
    12001 tctcgcggaa atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa
    12061 ttgtcaattt taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg
    12121 atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc
    12181 gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac
    12241 caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc
    12301 ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg
    12361 gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg
    12421 tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc
    12481 agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc
    12541 acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc
    12601 ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta
    12661 ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat
    12721 cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc
    12781 atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa
    12841 aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc
    12901 agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa
    12961 caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt
    13021 ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat
    13081 agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg
    13141 acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct
    13201 gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc
    13261 gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc
    13321 cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc
    13381 ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc
    13441 tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt
    13501 ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc
    13561 cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa
    13621 gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt
    13681 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    13741 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    13801 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    13861 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    13921 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    13981 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    14041 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    14101 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    14161 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    14221 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    14281 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    14341 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    14401 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    14461 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    14521 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    14581 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    14641 gaaaccacaa ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
    14701 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
    14761 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa
    14821 aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat
    14881 tgatatggcc atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct
    14941 caacacgatt ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag
    15001 ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct
    15061 aaatcgcgcc agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg
    15121 cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc
    15181 tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga
    15241 aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg
    15301 aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg
    15361 catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt
    15421 acccaattat gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt
    15481 ccatgcaagt ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg
    15541 ccttgccccc tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt
    15601 atcagtattg tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc
    15661 ttggtagctt tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt
    15721 ttcatacctc ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc
    15781 ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc
    15841 ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc
    15901 aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc
    15961 acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc
    16021 ccggcaatgg caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag
    16081 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg
    16141 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac
    16201 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa
    16261 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa
    16321 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca
    16381 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt
    16441 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg
    16501 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg
    16561 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc
    16621 cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc
    16681 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt
    16741 gagcgtcgga aaggcgc
//
