LOCUS       sequence-246719-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enocdes a histone H2B fused to the SunTag for single molecule 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-H2B-SunTag24x_v1
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             788..1165
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             1235..1300
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1316..1381
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1397..1462
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1478..1543
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1559..1624
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1640..1705
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1721..1786
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1802..1867
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1883..1948
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1964..2029
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2045..2110
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2126..2191
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     2207..2229
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     2230..2252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             2255..2320
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2336..2401
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2417..2482
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2498..2563
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2579..2644
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2660..2725
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2741..2806
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2822..2887
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2903..2968
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2984..3049
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3065..3130
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3146..3211
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     misc_feature    3282..3834
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3426..3443)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3653..3672
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3846..4445
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3846..3865)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4342..4362
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4483..4500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4489..4713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4759..5187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4846..4865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5056..5077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5196..5216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5201..5530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5381..5516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5443..5462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5578..5625
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5644..6018
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6148..6269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6185..6204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6239..6258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6318..6334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6318..6334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6331..6353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6342..6358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6366..6372,6373..6390,6391..6396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6411..6432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8908..8927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccggc gcgccaccgg
      781 tgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc
      841 ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta
      901 ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa
      961 ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc
     1021 ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc
     1081 cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc
     1141 catcaccaag tacaccagcg ctaaggatcg atccggtgga ggtggaggtc ctgcaggttc
     1201 gaaggtacaa agcggtccgg gaggttctgg aggattgttg cccaaaaact accacttgga
     1261 gaacgaggtc gcaaggttga aaaaattggt cggtgagcgt ggcggctcag gcggccttct
     1321 tccaaaaaat tatcatcttg aaaatgaagt tgctagactt aaaaaacttg ttggagaaag
     1381 aggcgggagc ggcggcctgc tgcccaagaa ctaccacctg gagaacgagg tcgcccgcct
     1441 gaagaagctg gtcggcgagc gcggtggttc tggaggtttg ctcccaaaga actaccactt
     1501 ggaaaacgaa gtcgcaagat tgaagaagtt ggtcggtgaa agaggaggaa gcggaggatt
     1561 attacctaaa aattatcatt tggaaaatga agttgctaga ttgaagaagt tggttggaga
     1621 gagagggggg tctggaggac tactaccgaa aaactaccac ctagagaacg aggtggcccg
     1681 actaaaaaag ctagtgggcg agcgcggcgg cagtggcggc ctcctcccca agaattacca
     1741 cctcgaaaat gaagtagcaa ggctcaagaa gctcgtagga gaaagaggtg gttccggggg
     1801 actccttcca aaaaactatc atcttgaaaa cgaagttgca cgtcttaaaa aacttgttgg
     1861 cgaacgtggc ggctctggcg ggctgctgcc caagaattac cacctggaaa atgaagtggc
     1921 ccgcctgaag aagctggtgg gcgaacgcgg aggcagtgga ggcctactac cgaagaacta
     1981 ccacctagag aacgaggtgg cacgcctaaa gaagctagtg ggcgagcgcg gcggctcggg
     2041 tggtttatta cctaagaatt atcatttaga aaatgaagta gctcgattaa agaagttagt
     2101 aggtgaacgt ggtgggtctg gtggtcttct tccgaagaac taccaccttg agaacgaggt
     2161 agctcgtctt aagaaacttg taggtgagcg tggtggaagc ggtggtgggc tggcaagcca
     2221 cgtttggtgc cgggagctgc atgtgtcaga ggaacttttg ccaaagaatt atcatcttga
     2281 gaacgaagtg gctcgtctta agaaactcgt aggagaaaga ggtggcagtg gaggtctgct
     2341 tcccaagaat taccacctgg aaaatgaggt agctagactg aaaaagctcg taggcgagcg
     2401 cgggggaagt gggggattgc tgccaaaaaa ttatcatttg gagaacgaag tagcacgact
     2461 aaagaaatta gttggggaga gggggggatc gggtggctta ctcccgaaaa attatcatct
     2521 cgaaaacgaa gtggctcggc taaaaaagtt agttggagag cgtggcggtt ctggaggcct
     2581 attaccaaaa aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagc tggtcggaga
     2641 acggggaggc agtggtggcc tactacccaa gaattatcat cttgagaacg aggtagcgcg
     2701 tttgaagaag ctcgtcggtg aaagaggtgg ctcaggaggg ctgctcccaa agaactatca
     2761 tcttgaaaat gaggtcgctc gattaaaaaa gttagtgggc gagaggggag gcagtggtgg
     2821 gctacttcct aagaattacc acctcgaaaa cgaagtagct cgattaaaga aattggtcgg
     2881 tgagagaggt gggtcgggtg gcttactgcc gaaaaattat catctggaaa atgaggtagc
     2941 gagactaaaa aagctcgtag gtgagagagg gggttctggc ggcttgctac caaaaaatta
     3001 tcatcttgag aacgaagttg ctaggctcaa aaagctggtt ggagagcgcg gcggctcagg
     3061 cggtctgtta cccaaaaact accacttgga aaatgaggtt gccagattga agaaactggt
     3121 cggcgagcgc ggtggaagtg gaggactact tcctaaaaat tatcatttgg agaacgaagt
     3181 cgctagactg aaaaagttgg ttggggaacg aggagggtct ggcggcaagc tagtcgaatt
     3241 cgacacgtag gctagcttag taggcggccg ccccccccct aacgttactg gccgaagccg
     3301 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3361 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3421 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3481 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3541 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3601 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3661 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3721 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3781 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3841 caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     3901 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     3961 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4021 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4081 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4141 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4201 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4261 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4321 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4381 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4441 cctgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4681 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4741 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4801 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4981 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5041 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5101 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5161 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5221 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5281 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5341 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5461 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5521 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     5581 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5641 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5701 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5761 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     5821 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     5881 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     5941 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     6001 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     6061 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6121 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6241 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6301 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6901 gtggcgcttt ctcatagctc acgctgtagg tatcttcctc gctcactgac tcgctgcgct
     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8881 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8941 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9001 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     9061 tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
