LOCUS       sequence-246721-       10176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of UPRT and CD along with reporter 
            RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Basnet H, Tian L, Ganesh K, Huang YH, Macalinao DG, Brogi E, Finley 
            LW, Massague J
  TITLE     Flura-seq identifies organ-specific metabolic adaptations during 
            early metastatic colonization.
  JOURNAL   Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627.
  PUBMED    30912515
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-26"; volume: "8. pii: 43627. doi"; pages: " 
            10.7554/eLife.43627"
COMMENT     Sequence Label: pCMVtight-UPRT-T2A-RFP-IRES-CD
FEATURES             Location/Qualifiers
     source          1..10176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1714..2028
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1722..1740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1758..1776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1793..1811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1829..1847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1865..1883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1936..1954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2001..2025
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2106..2130
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2193..2219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2979..3032
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3039..3734
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     misc_feature    3757..4329
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     4151..4170
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4353..4362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4841..4865)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4896..5484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4949..4969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5367..5378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5535..5555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5540..5869
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5720..5855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5917..5964
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5983..6381
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6468..6701
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6773..6894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6810..6829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6864..6883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6934..7069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7089..7108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7090..7108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7118..7135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7118..7134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7127..7149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7276..7731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7363..7382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7573..7594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7757..7861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7862..7930,7931..8722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8080..8099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8893..9481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9382..9401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9635..9652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9769..9790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9805..9810,9811..9828,9829..9835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9843..9859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9848..9870
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9867..9883
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9867..9883
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9902..9922
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9904..9922
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9950..10176
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagttta ctccctatca gtgatagaga
     1741 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     1801 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     1861 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     1921 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     1981 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga
     2041 gctcggtacc cggggatcca ccggttcgtc gactagtcca gtgtggtgga attctgcaga
     2101 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatct
     2161 cgagctcaag cttcgaagcc gccaccatgg cctaccccta cgatgtgccc gattacgcca
     2221 ctagtgctag cagatctgcg gccaacatgg cgcaggtccc agcgagcgga aagctccttg
     2281 tcgatccccg atattcgaca aacgaccagg aagaaagcat tctccaggac atcatcacga
     2341 ggtttcccaa tgtggtgctc atgaagcaga cggctcagct tcgagcgatg atgaccatca
     2401 ttcgtgataa agaaacaccg aaggaagaat tcgtcttcta cgccgaccgc ctgattcgcc
     2461 tcctcatcga agaagctttg aacgaactgc cgttcgaaaa gaaggaagtg acaacccctc
     2521 tggatgtgtc ataccatgga gtttccttct attccaagat ctgtggcgtc tcgattgtga
     2581 gagctggcga gtcgatggaa agcggcttgc gggcagtttg ccgcggctgc cgcatcggga
     2641 aaatcctcat ccagagagac gaaacaactg cggagcctaa gctgatctac gagaagctgc
     2701 ctgccgacat tagagatcgc tgggtgatgc tgctagatcc gatgtgcgcg acggcgggca
     2761 gtgtgtgcaa agcgatcgag gtcctcctga ggctcggcgt gaaggaagag agaatcattt
     2821 tcgtcaacat tttggctgct ccccaaggca ttgaacgtgt tttcaaggaa tacccgaaag
     2881 tccgcatggt cactgctgct gttgacatct gcctgaactc gaggtactac atcgtccccg
     2941 gcattggtga tttcggtgac cggtactttg gaaccatgga gggcagagga agtcttctaa
     3001 catgcggtga cgtggaggag aatcccggcc cttccgggat gagcgagctg atcaaggaga
     3061 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     3121 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
     3181 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
     3241 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     3301 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     3361 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
     3421 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
     3481 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
     3541 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
     3601 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
     3661 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
     3721 tggggcacag ataaggatcc gcggccgcaa attccgcccc tctccctccc ccccccctaa
     3781 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     3841 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3901 gagcattcct aggggtcttt cccctctcgc caagggaatg caaggtctgt tgaatgtcgt
     3961 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4021 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4081 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4141 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4201 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4261 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4321 acgataatac catggccacc gagtacttcg aagccaccat ggtgacaggg ggaatggcaa
     4381 gcaagtggga tcagaagggt atggacattg cctatgagga ggcggcctta ggttacaaag
     4441 agggtggtgt tcctattggc ggatgtctta tcaataacaa agacggaagt gttctcggtc
     4501 gtggtcacaa catgagattt caaaagggat ccgccacact acatggtgag atctccactt
     4561 tggaaaactg tgggagatta gagggcaaag tgtacaaaga taccactttg tatacgacgc
     4621 tgtctccatg cgacatgtgt acaggtgcca tcatcatgta tggtattcca cgctgtgttg
     4681 tcggtgagaa cgttaatttc aaaagtaagg gcgagaaata tttacaaact agaggtcacg
     4741 aggttgttgt tgttgacgat gagaggtgta aaaagatcat gaaacaattt atcgatgaaa
     4801 gacctcagga ttggtttgaa gatattggtg agtagtctag acccagcttt cttgtacaaa
     4861 gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     4921 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4981 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5041 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5101 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5161 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5221 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5281 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     5341 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5401 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5461 tctccctttg ggccgcctcc ccgcctggaa ttctgcagat atccggttag taatgagttt
     5521 ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5701 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5821 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5881 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     5941 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
     6001 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     6061 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     6121 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     6181 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     6241 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     6301 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     6361 ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac caatgactta
     6421 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     6481 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6721 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6781 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6841 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6901 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6961 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7021 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     7081 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     7141 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7201 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7261 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7321 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7381 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7441 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7501 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7561 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7621 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7681 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7741 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7801 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7861 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7921 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7981 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8041 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8101 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8161 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8221 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8281 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8341 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8401 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8461 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8521 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8581 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8641 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8701 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8761 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8821 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9001 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9301 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9541 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9601 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9661 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9721 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9781 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9841 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9901 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     9961 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
    10021 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
    10081 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
    10141 gatattgtat ttaagtgcct agctcgatac ataaac
//
