LOCUS       sequence-246736-        8936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of V5 dDispatched WT HA in Drosophila cells lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, 
            Pruett-Miller SM, Ogden SK
  TITLE     Cleavage activates dispatched for Sonic Hedgehog ligand release.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678.
  PUBMED    29359685
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 31678. doi"; pages: " 
            10.7554/eLife.31678"
COMMENT     Sequence Label: pAc V5 dDispatched WT HA
FEATURES             Location/Qualifiers
     source          1..8936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2852..2893
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6239..6265
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6328..6462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6352..6371)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6406..6425
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7063..7651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7143..7162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7822..8613,8614..8682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8445..8464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8683..8787)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8855..8873
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccgatg ttgtgcttcg actcggagag gatgaactgg tactaccacg tcctggccag
     2581 gcgtccctac ctggtggtcg tctccattgc tgtctactgt gtcgcttgca tcatcgtggc
     2641 cctcgtcctg aacaagctgc cggacttcag tgatcccacc ttgggcttcg aaacccgcgg
     2701 caccaaaatt ggagaacgac tgacggcctg gtacaacctg ctgcaagaga ctgaccacca
     2761 tggtgctctt ttctcaaatc catcggatct gtgggagagg agacgggtag agcagggcta
     2821 tgtggagacg aagctgcatc cgaatcacgt gggtaagcct atccctaacc ctctcctcgg
     2881 tctcgattct acgcacgtga ggcgacgcaa aaacaagcac aagaacagaa acaagaacaa
     2941 gcgacgcaag gaacaaaatc aaagcagcca cgagcatcac gatgtggcgc agaagatgat
     3001 gcagttcaaa aaacggctga aggctactag ttctccctct ccaaatctcg gctttgacac
     3061 ctggatcggg gacagtggag tatttcgcga ctacgagatt acaaacgaca gcgcttcctc
     3121 ctctttggaa cccacgcgac gcactgagca aatcgaatat ggccacaaca ccacctcagt
     3181 ggacgaggaa gagcatcagc agcgggtgca gaccaagaag agcacttggc ggctcctaaa
     3241 gcaagccgcc accttgccca ccgacggttg ggcagacatg catcgtcgcc aacccatcga
     3301 gggattcttt tgcgattcat cgccccggaa ggagtactct cattttgtgg tccagcggat
     3361 cggtcccaac gccaccgatt ctctatttga tttgaacgga ctgctggcca tgtgtcagtt
     3421 gcaggatcag attaccgaag tccccagcta ccgggcattt tgcgagccgg agatgcttac
     3481 caccgagtgc tgccggccat ggtcactgcc caactacgcc gccatgctgg ccaacaaaag
     3541 ttcctgtttc gaccttacca cggaggatgt tacttcgctg cacacgctgc tcttgggctg
     3601 ctatgagtac ttccatgacc taaagatgga caaccactgc aacgaaatac cgcattgtcg
     3661 tgctcctgaa gagtgcaagc ggctaaatat cgtctttaac gtcctgaatt ttctcaccga
     3721 ctttagtttc atcaagtcta atgactcgaa tgtatacctg aaatacgcca tgatttttat
     3781 acccgtggcg caatccaacc gcttgctgcc actgttccac gaatgggaag acgtggagct
     3841 gatcaacgag cttgtggaag tggttgcgat ggacttgggc ctcgaaaacg aactctttaa
     3901 cgagctgctg ctgacggacg tgtggctagt ttcgctggga gggacattcg tcatggctag
     3961 cgtgtggttg tacacgggat cggcattcat caccttgatg tcctgcgtag ccatatgctt
     4021 ttcgctagga ctagcgtact ttttttacgc catcgtgctg gagtttgagt tcttccccta
     4081 tatgaacctt ctggccgtgg tagtaataat tggcattgga gcagacgacg tatttctgtt
     4141 tcttaagatc tggcactgtg tgctgaccga gaggttcagc aatcgatgta ccttgaccac
     4201 tcagtcccag agtgctcttc ccaccctgga gaacagtgat cacacggagt cactggaaaa
     4261 cataatggcg ctgactatgc gacatgccgc tgcctccatg ttcgttacct cacttaccac
     4321 cgccggcgcc ttttatgcct cctacagcag ctctataaca gctataaagt gctttgggat
     4381 ttttgcggga actgtcgtgg tgaccaacta cttactaatg ataacttggt tgcctgcatc
     4441 ggtctccatc atggaacgac tatttgccac aaggatgtcc tgccatcatc cgatgtcaat
     4501 aaagctgatc cacgcctgca agaagtcaat taaccgattt tgtcagatgt ttgaggagtg
     4561 catcacgaaa agcatcatga actatgccta tctctggctg ctgatctttg gggctctagg
     4621 cgcgtccagt gccgtcattg tgttctggta tccaggactt cagttgccgg aaaaatcaca
     4681 cttccagctc tttgtgtcaa agcatccatt tgaggtttac tccagtctca agcagcagtt
     4741 ctggttcgag aaaccattgc aggcgtacga gaacttcaag atgcacatgc atttcgtctg
     4801 gggcgttcaa gcggtggacg acggcgacta tacgaacccc aactcatacg gccacctgca
     4861 ctatgacaat aattttaacg tatccagcag gccggcacaa ctctggatcc ttgatttttg
     4921 ccagagtgtg cgccagcaac ccttttacaa agagactctc ggcatgctgt tgcccaattg
     4981 tttcattgaa aatcttatcg actatatgaa gcgcagatgc atcgatgata tggacagtac
     5041 caggaaagac cgttcaccct gctgtgacgc acagtttccc tttgagccgc acatatttga
     5101 gtactgcttg ccacaaagca tatccaacat gtatgacact acatttttcc ggcccggcgt
     5161 ggcaggaccc aagtttgcag aggctccccg actggagact gaggattacc taggaatgag
     5221 tggaaatgag agtgcagagt acagcaccaa cggatcattt acaccgctac tagtcaaagc
     5281 cctggtcatc gagttcgagt ccaacgtggc ctacagcacc atctacgcaa atattaggca
     5341 gttctacgag tctgtagagc actggtttca gatgcagtta aaaacggcac cgccggaact
     5401 tcaaggagga tggttcacga gcgacctgaa attttacaat gtgcaggaca ccctttcgca
     5461 cgacaccttt gttgccattt gtctggccat ggctgcatca cttgcagtgc tcctatgctt
     5521 caccgtaaac atattgattt ccatctacgc cgtactaacc gtgtcgctaa gtatttttaa
     5581 caccgtggct gtgctcatcc tgctcggctg gcagcttaac atcttggaga gcattgcggt
     5641 gagcacggct atcggtctgg ctgtggactt tagcctacat tacggtattc actaccggat
     5701 gtccccggtt aaggagagat tggcagccac acagtttgta ctatcccgca tcattggacc
     5761 cacagtgatg gcggccacca caactggtct agctggcgga atcatgatgg catccaacat
     5821 attgccctac atacagattg gcgtcttcct ggtcgttgtc atgatcgtta gctggttcta
     5881 tgccactttc tttctaatga gtctgcttcg ggttgccggt cctcagcatg gttttctgga
     5941 actcaagtgg ccgttgtgga gcaagcgaag cagtggcagc agcaagttct acgagcggaa
     6001 acccagccaa gtgatcgcca gcgagcagct gctgaccccc acaagctcgg ccatcgttga
     6061 gttggcgaac tcggagacgc acgaactgga gtccctaaac tccaacagcc tgatcaaaac
     6121 catttcgggc atcgaaagcg cccacgcatt gtcctcgctg ccgagggact tcgagcactc
     6181 attccagacg atgcacgagt gcaaatatca aacgtatccg tctacatcca atggtaccta
     6241 cccatacgac gtcccagact acgcttgaga tatccagcac agtggcggcc gctcgagtct
     6301 agagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt
     6361 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     6421 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     6481 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     6541 tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc
     6601 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     6661 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     6721 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     6781 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     6841 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     6901 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     6961 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca
     7021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7501 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8101 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     8161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     8461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8821 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8881 tcttcaagaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaaat
//
