LOCUS       sequence-246833-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Ub fused to GyrA intein at the C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S
  TITLE     Efficient generation of hydrazides in proteins by RadA split intein.
  JOURNAL   Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160.
  PUBMED    31265209
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160."
COMMENT     Sequence Label: Ub-GyrA
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      213..299
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     434..453
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        434..452
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    453..477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             492..514
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             522..749
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 cntaaaantn ccaggattaa ttccccaggc ntcaatnaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttntct ntgtttgtcg gtgaacgctn tctgagtagg acaaatccgc
      121 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
      181 cataaactgc caggaattaa ttccccaggc atcaaataaa acgaaaggct cagtcgaaag
      241 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      301 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
      361 cgccataaac tgccaggaat tggggatcgg aattaattcc cggtttaaac cggggatctc
      421 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      481 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcagatc ttcgtcaaga
      541 cgttaaccgg taaaaccata actctggaag ttgaaccatc cgataccatc gaaaacgtta
      601 aggctaaaat tcaagacaag gaaggaattc cacctgatca acaaagattg atctttgccg
      661 gtaagcagct cgaggacggt agaacgctgt ctgattacaa cattcagaag gagtcgacct
      721 tacatcttgt cttaagacta agaggtggtt gcatcacggg agatgcacta gttgccctac
      781 ccgagggcga gtcggtacgc atcgccgaca tcgtgccggg tgcgcggccc aacangnnnn
      841 ngcc
//
