LOCUS       sequence-246928-       15604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15604)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX072
FEATURES             Location/Qualifiers
     source          1..15604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5718..5742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5771..5860
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5894..6146
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6575..6827)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7295..8320)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7295..8320)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8330..8674)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8816..8833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8816..8832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8825..8847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8972..8991)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9182..9203
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9450..9474
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11070..12218)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12517..13311)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLQGIKPDWEKIKYYILLDELF"
     mobile_element  13503..14270
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14963..15594
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gaaattcaca tggctcacca
     4621 tctgtttgag agacgcccca tatacaagtc caaagctcct gcttccaaat ggttcaaaga
     4681 atgggtcccg caagatgttg tagcaactgg tgggaagtgc tacctcttaa agtgggtgaa
     4741 tgaggccaca ctgaaagctc tgaaggagaa gcccaaagag ccagaggtac ccctgccaga
     4801 gccggagcca actactgaag ttcttttcct ttgtagctat gacagttgtg gaaagacatt
     4861 tattgatgct ggggccttga ggaagcattc tcacatccac ggggagaggc agtatgtatg
     4921 tcattatgag ggttgtggaa agaaattttt ggatagctcc aagctaaaac ggcattttct
     4981 tattcacact ggggaaagag actatgtatg tcctcatgaa ggctgtggca aggcattctc
     5041 actggacttt aacttaagat cacacatgaa aacacactcc caggagaact atcatatctg
     5101 cccgtatcca gaatgtggaa agcgatatgc acatgagtac aagcttaaaa atcatgttgc
     5161 atctagtcac cagaagaact ctacacctga tgctccaaag tataccccac ctgtggagaa
     5221 gccaatgaaa acaccgaaat cttcttcagc agcttatgcc tcatcatcat ctgaccgccc
     5281 ttatagttgt ccgtacgaag gatgtgacaa atcttacatc catgaataca agctgaatct
     5341 tcatttaagg aacatgcacc ctggtcattt tccagaggat aatgcaaaaa atgttcaatc
     5401 tactgctgac aatgagatgg atgaaggcag tgatcaagac gcgtatgctg caaaacgtgg
     5461 aaacggcaaa attcagaaac atagcaggcc aaaaccaagc ttaaagcagc cacctttaaa
     5521 ggttgcacgg aaaagctcta gtgcttctcc agccaatata agtatggcac caaagaagcc
     5581 ttggccggtt aaagaagaga actacaacga agaagatagt gaagaaacag aagaggaacg
     5641 ggatgatgtt ggcgatggat ggagatatgg tgaaaacgat gacgacgacg aagagactga
     5701 agaagatgag cccggggacc cagctttctt gtacaaagtg gttgataaca gcgggttaat
     5761 taacatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5821 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag
     5881 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5941 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     6001 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     6061 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     6121 cgcggtgtca tctatgttac tagatcggga attagcttca tcaacgcaag acatgcgcac
     6181 gaccgtctga caggagagga atttccgacg agcacagaaa ggacttgctc ttggacgtag
     6241 gcctatttct caggcacatg tatcaagtgt tcggacgtgg gttttcgatg gtgtatcagc
     6301 cgccgccaac tgggagatga ggaggctttc ttggggggca gtcagcagtt catttcacaa
     6361 gacagaggaa cttgtaagga gatgcactga tttatcttgg cgcaaaccag caggacgaat
     6421 tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg gcatgagcag aaacttccaa
     6481 ttcgaaacag tttggagagg ttgtttttgg gcataccttt tgttagtcag cctctcgatt
     6541 gctcatcgtc attacacagt accgaagttt gatcgatcta gtaacataga tgacaccgcg
     6601 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     6661 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     6721 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     6781 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttcgacgcac
     6841 tccttcttta ctccaccatc tcgtccttat tgaaaacgtg ggtagcacca aaacgaatca
     6901 agtcgctgga actgaagtta ccaatcacgc tggatgattt gccagttgga ttaatcttgc
     6961 ctttccccgc atgaataata ttgatgaatg catgcgtgag gggtatttcg attttggcaa
     7021 tagctgcaat tgccgcgaca tcctccaacg agcataattc ttcagaaaaa tagcgatgtt
     7081 ccatgttgtc agggcatgca tgatgcacgt tatgaggtga cggtgctagg cagtattccc
     7141 tcaaagtttc atagtcagta tcatattcat cattgcattc ctgcaagaga gaattgagac
     7201 gcaatccaca cgctgcggca accttccggc gttcgtggtc tatttgctct tggacgttgc
     7261 aaacgtaagt gttggatccc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     7321 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7381 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7441 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7501 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7561 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7621 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7681 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7741 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7801 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7861 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7921 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7981 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     8041 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     8101 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     8161 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     8221 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     8281 acttctcgac agacgtcgcg gtgagttcag gctttttcat atcggggtcg tcctctccaa
     8341 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     8401 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     8461 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8521 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     8581 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     8641 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     8701 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga cggatctcta
     8761 ggacgcgtcg acgcgtcgac gcgtcgacgc gtcgacgcgt cctagaagct aattcactgg
     8821 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8881 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8941 cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc ttcctttctc
     9001 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9061 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     9121 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9181 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     9241 ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc
     9301 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     9361 tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat
     9421 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     9481 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     9541 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     9601 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     9661 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat
     9721 ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag
     9781 gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg
     9841 aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc ccctatccat
     9901 tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct gatgcatccc
     9961 cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa ctgtaggttc
    10021 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
    10081 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
    10141 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
    10201 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
    10261 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
    10321 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
    10381 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
    10441 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
    10501 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
    10561 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
    10621 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
    10681 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
    10741 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
    10801 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
    10861 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
    10921 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
    10981 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
    11041 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
    11101 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
    11161 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
    11221 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
    11281 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
    11341 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
    11401 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
    11461 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
    11521 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
    11581 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
    11641 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
    11701 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
    11761 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
    11821 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
    11881 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
    11941 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
    12001 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
    12061 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
    12121 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
    12181 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
    12241 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
    12301 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
    12361 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
    12421 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
    12481 tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat
    12541 ataatatttt attttctccc aatcaggctt gatcccctgt aagtcaaaaa atagctcgac
    12601 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
    12661 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
    12721 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
    12781 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
    12841 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
    12901 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
    12961 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
    13021 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
    13081 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
    13141 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
    13201 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
    13261 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
    13321 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
    13381 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
    13441 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta
    13501 tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
    13561 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
    13621 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
    13681 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
    13741 aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta
    13801 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
    13861 tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc
    13921 gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg
    13981 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
    14041 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
    14101 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
    14161 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
    14221 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caattatgat
    14281 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
    14341 tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct tgccccctga
    14401 aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc agtattgtca
    14461 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
    14521 aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc atacctcggt
    14581 ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg aacacgagca
    14641 cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc cccgccatga
    14701 agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg ccgccgccct
    14761 cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc
    14821 cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg gcaatggcaa
    14881 ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
    14941 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
    15001 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
    15061 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
    15121 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
    15181 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
    15241 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
    15301 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
    15361 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
    15421 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
    15481 caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
    15541 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcggaaag
    15601 gcgc
//
