LOCUS       sequence-246966-       16706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16706)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX087
FEATURES             Location/Qualifiers
     source          1..16706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6820..6844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6873..6962
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6996..7248
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7677..7929)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8397..9422)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8397..9422)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9432..9776)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9918..9935)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9918..9934)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9927..9949)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10074..10093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10284..10305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10552..10576
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12172..13320)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13619..14413)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14605..15372
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16065..16696
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat gtctttctca tctggggcag tctctggggt ccgctgagcc
     5881 tgcccgcgat aaatgatatg tatcagccca aaagcgttcc aggttcaagc ttagcccaca
     5941 acaatgcagc agtgcatatt cagtcattag actgtgatga tggctcaatg gatccgacca
     6001 gtggaggcaa taccgtcaac aatcctagtc ttgcttcaaa gcaacgtctg cgttggacca
     6061 atgagcttca tgaacgattt gttgatgctg tcgcacaact tggtggccca gatagagcta
     6121 caccgaaagg cgttcttaga gtcatgggtg tacaaggcct aaccatttac catgtaaaaa
     6181 gccatttaca gaaatatcga cttgctaaat atcttccaga ttcctcattt gatgggaaaa
     6241 accctgataa gaaagaatcg agggatatgc tatcaagttt ggatggttca tcaggagtgg
     6301 aaatcactac ggctctaaag ctgcagatgg aggtgcaaaa gcgactgcac gagcaactgg
     6361 aggtccagaa acagctacac cttagaatag aagcccaagg gaagtatttg aagaagataa
     6421 tcgaagagca acagcggctc agtggagttc tttcagaagt tcctggttct ggggtcgctg
     6481 ctcctccaac tggtgacaat ggcccagaat ctgataatag gagtgatcca gggactcctg
     6541 caccaacatc tgaatcccct aatgttgaga agcatgtaaa agcacatgcc ccaaccaaga
     6601 gcctttctgt ggacgaacca ttctccttgc agcatgagcc tctcactcca gattctgatt
     6661 gcccagagtc gtctccaatg gagagcccta atggcgagag gtcatcgaag aaacaaagag
     6721 tgggcacaaa tgtagccttt gcaaaagacg atgtggcact tacacatcag atattggaat
     6781 caagcttgag cccaggatac cagtcgagga cccagcttga cccagctttc ttgtacaaag
     6841 tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg
     6901 gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg
     6961 ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     7021 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     7081 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     7141 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     7201 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt
     7261 catcaacgca agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga
     7321 aaggacttgc tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt
     7381 gggttttcga tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg
     7441 cagtcagcag ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt
     7501 ggcgcaaacc agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt
     7561 cggcatgagc agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct
     7621 tttgttagtc agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc
     7681 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     7741 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     7801 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     7861 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     7921 tgaacgatct gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg
     7981 tgggtagcac caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat
     8041 ttgccagttg gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg
     8101 aggggtattt cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat
     8161 tcttcagaaa aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt
     8221 gacggtgcta ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat
     8281 tcctgcaaga gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg
     8341 tctatttgct cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat
     8401 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     8461 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     8521 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     8581 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     8641 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     8701 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     8761 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     8821 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     8881 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     8941 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     9001 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     9061 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     9121 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     9181 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     9241 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     9301 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     9361 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     9421 atatcggggt cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     9481 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     9541 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     9601 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     9661 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     9721 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     9781 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     9841 ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg acgcgtcgac gcgtcgacgc
     9901 gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9961 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10021 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt
    10081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10201 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10321 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
    10381 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    10441 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca
    10501 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
    10561 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
    10621 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
    10681 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
    10741 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
    10801 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
    10861 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa
    10921 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga
    10981 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc
    11041 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca
    11101 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
    11161 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
    11221 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
    11281 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
    11341 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
    11401 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
    11461 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
    11521 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
    11581 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
    11641 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
    11701 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
    11761 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
    11821 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
    11881 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
    11941 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct
    12001 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
    12061 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
    12121 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
    12181 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
    12241 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
    12301 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
    12361 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
    12421 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
    12481 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
    12541 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
    12601 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
    12661 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
    12721 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
    12781 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
    12841 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
    12901 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
    12961 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
    13021 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
    13081 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
    13141 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
    13201 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
    13261 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
    13321 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
    13381 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
    13441 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
    13501 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat
    13561 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact
    13621 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    13681 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    13741 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    13801 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    13861 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    13921 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    13981 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    14041 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    14101 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    14161 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    14221 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    14281 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    14341 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    14401 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    14461 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    14521 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    14581 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg
    14641 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
    14701 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    14761 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    14821 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    14881 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    14941 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    15001 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    15061 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    15121 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    15181 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    15241 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    15301 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    15361 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca
    15421 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg
    15481 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata
    15541 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
    15601 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
    15661 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
    15721 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta
    15781 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg
    15841 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc
    15901 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact
    15961 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc
    16021 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    16081 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    16141 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    16201 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    16261 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    16321 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    16381 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    16441 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    16501 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    16561 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
    16621 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
    16681 cagcccggtg agcgtcggaa aggcgc
//
