LOCUS       sequence-246968-       15871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15871)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX089
FEATURES             Location/Qualifiers
     source          1..15871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5985..6009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6038..6127
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6161..6413
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6842..7094)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7562..8587)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7562..8587)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8597..8941)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9083..9100)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9083..9099)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9092..9114)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9239..9258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9449..9470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9717..9741
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11337..12485)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12784..13578)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLQGIKPDWEKIKYYILLDELF"
     mobile_element  13770..14537
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15230..15861
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg cttgctgtgt catctttgag
     4621 taacacaagg aacaaagatg aaaggggtaa cgagatggaa agtttcacga tcggaggagg
     4681 tgacgatttt ccagatttta tgggtgagaa tttgctcgac ggtattgatt ttgatgacct
     4741 ttttgttggg atcaatgacg gagatttgct agctgatttg gagatggacg ctgagatttt
     4801 tgctgaattt tcagttagta gtggcgatga atctgacgtg aacaactata atatctctac
     4861 tcctactact ataaaaaatg ttgaacaaga gagttgttta agaaaagaag tggaaaaggc
     4921 atcatcagtt tcagcttcgg atcttggttc ggggttaacg agcccaaatt atcagggaga
     4981 agaaattgtt agtcaaaaga gtgaagaatc tgctccacca gtgaatcaga atcctgtgca
     5041 taaagaaagt gataaaggca aaaagtcatc tgctcaatcc aagaataatc ctcaagggaa
     5101 gaggaaagtt aaggtggatt ggacgccaga attgcacagg agatttgtac aagcagtaga
     5161 gcagctaggt atagataagg cagtcccatc tagaattttg gaaattatgg gaatcgattg
     5221 tctcactcgc cataacattg ccagccatct ccagaaatat cgatcccata ggaagcattt
     5281 acttgcaaga gaagcggagg cggcgagctg gagtcaaaga aggcaaatct acggcggtgc
     5341 agcaggcgga gggggaggag gaaagaaaga gatgaaccca tggcctgcgc cgactattgg
     5401 ctttcctcct cctcctcaga tggcaactcc aatgcctcat tttagaccct tacatgtatg
     5461 gggtcatcca tctttcgaac aatcatatat gcacatgtgg cctaagcatt tagcaccttc
     5521 accttctcct caccagcatc catcgccagt ttggccacct gctgttcatc ctcctcctca
     5581 tcttcatcct gaaccacctg cagatccttc tttttggcat ccacatcatc aaccggtacc
     5641 aaattctctt accccaggaa ctacttactt tccagcacct atagcaccaa cgagatatcc
     5701 tgttcctcat ccagtcccag gcattccatc ccctgccgcc atgtacaaag tagaccccgg
     5761 cattggcgtt cgaactgtcg tcgccggagc tggacaacca cttcctaaac ctccttgtgg
     5821 ctttcatcct tcaaaggaga gcatagatgc ggctattgga gatgttttat caaagccatg
     5881 gctgccactt cccctcggac tgaaacctcc cgctgtggac agtgtgttgg gagaattaca
     5941 acgtcaaggg gtacctaaaa taccaccaac ttgtgcaccc ggggacccag ctttcttgta
     6001 caaagtggtt gataacagcg ggttaattaa catcttttac ccatacgatg ttcctgacta
     6061 tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
     6121 ttacgctgct cagtgcagcg cttagagctc gaatttcccc gatcgttcaa acatttggca
     6181 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     6241 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     6301 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     6361 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     6421 agcttcatca acgcaagaca tgcgcacgac cgtctgacag gagaggaatt tccgacgagc
     6481 acagaaagga cttgctcttg gacgtaggcc tatttctcag gcacatgtat caagtgttcg
     6541 gacgtgggtt ttcgatggtg tatcagccgc cgccaactgg gagatgagga ggctttcttg
     6601 gggggcagtc agcagttcat ttcacaagac agaggaactt gtaaggagat gcactgattt
     6661 atcttggcgc aaaccagcag gacgaattag tgggaatagc ccgcgaatat ctaagttatg
     6721 cctgtcggca tgagcagaaa cttccaattc gaaacagttt ggagaggttg tttttgggca
     6781 taccttttgt tagtcagcct ctcgattgct catcgtcatt acacagtacc gaagtttgat
     6841 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     6901 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     6961 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     7021 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     7081 atgtttgaac gatctgcttc gacgcactcc ttctttactc caccatctcg tccttattga
     7141 aaacgtgggt agcaccaaaa cgaatcaagt cgctggaact gaagttacca atcacgctgg
     7201 atgatttgcc agttggatta atcttgcctt tccccgcatg aataatattg atgaatgcat
     7261 gcgtgagggg tatttcgatt ttggcaatag ctgcaattgc cgcgacatcc tccaacgagc
     7321 ataattcttc agaaaaatag cgatgttcca tgttgtcagg gcatgcatga tgcacgttat
     7381 gaggtgacgg tgctaggcag tattccctca aagtttcata gtcagtatca tattcatcat
     7441 tgcattcctg caagagagaa ttgagacgca atccacacgc tgcggcaacc ttccggcgtt
     7501 cgtggtctat ttgctcttgg acgttgcaaa cgtaagtgtt ggatcccggt cggcatctac
     7561 tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     7621 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     7681 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     7741 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     7801 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     7861 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     7921 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
     7981 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
     8041 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
     8101 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
     8161 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
     8221 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
     8281 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
     8341 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
     8401 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
     8461 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
     8521 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
     8581 ttttcatatc ggggtcgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     8641 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     8701 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     8761 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     8821 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     8881 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     8941 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     9001 gtgctccacc atgttgacgg atctctagga cgcgtcgacg cgtcgacgcg tcgacgcgtc
     9061 gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     9121 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     9181 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct
     9241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9361 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9481 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca
     9541 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     9601 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     9661 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     9721 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     9781 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     9841 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     9901 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     9961 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
    10021 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
    10081 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
    10141 gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa
    10201 gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa
    10261 gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta
    10321 ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg
    10381 catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc
    10441 cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac
    10501 ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag
    10561 cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag
    10621 gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag
    10681 cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa
    10741 caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca
    10801 gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg
    10861 cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag
    10921 atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat
    10981 ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc
    11041 catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag
    11101 ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg
    11161 agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc
    11221 gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga
    11281 aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
    11341 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
    11401 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
    11461 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
    11521 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
    11581 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
    11641 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
    11701 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
    11761 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
    11821 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
    11881 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
    11941 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
    12001 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
    12061 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
    12121 aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
    12181 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
    12241 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    12301 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg
    12361 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    12421 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    12481 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    12541 tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    12601 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    12661 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    12721 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag
    12781 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    12841 cccctgtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    12901 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    12961 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    13021 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    13081 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    13141 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    13201 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    13261 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    13321 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    13381 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    13441 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    13501 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    13561 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    13621 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    13681 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    13741 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta
    13801 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
    13861 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    13921 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
    13981 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
    14041 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
    14101 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
    14161 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
    14221 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
    14281 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
    14341 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
    14401 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
    14461 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
    14521 aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg
    14581 tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa
    14641 gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa
    14701 agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct
    14761 tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag
    14821 gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg
    14881 ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca
    14941 aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg
    15001 gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg
    15061 ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg
    15121 ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc
    15181 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
    15241 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    15301 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    15361 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    15421 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    15481 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    15541 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    15601 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    15661 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    15721 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
    15781 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    15841 gcaaccagcc cggtgagcgt cggaaaggcg c
//
