LOCUS       sequence-246970-       16933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16933)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX091
FEATURES             Location/Qualifiers
     source          1..16933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7047..7071)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7100..7189
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7223..7475
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7904..8156)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8624..9649)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8624..9649)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9659..10003)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10145..10162)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10145..10161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10154..10176)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10301..10320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10511..10532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10779..10803
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12399..13547)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13846..14640)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14832..15599
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16292..16923
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca tgctaactgt atcacctttg agctacaaaa ccaccaaaaa
     5881 tgacagggat aatcacatgg atagttttac gattcgagga gttgatgact tgccagattt
     5941 tgatgatggg aatttgctcg aaagcattga ttttgatgac attttcttgg ggatcaacgt
     6001 caacgatgaa atgttgccag atatggagat ggactcagag attcttgctg aattctcagt
     6061 tagtagtggc gacgaatctg atcatttgaa tagttataat actactacac caccactcca
     6121 tgtagaagaa cacaactcct ctacaaaaga agaagtggaa aaagcagtat cagatttgaa
     6181 tcagaagagt gaaaaatcta aacctaagtc aaaggagaag cccaagaatc cagaagggaa
     6241 gagaaaagtg aaggtggatt ggacgccaga gctacacagg agatttgtac aagcagtaga
     6301 gcaattaggt gtagataaag cagtcccatc gaggatttta gaacttatgg gtgttgatgg
     6361 tctcactcga cataacattg ctagccatct tcaaaaatat agagcccatc ggaaacattt
     6421 gcttgcgaga gaagcggagg cggcaagctg gagccaaaga aaacaaatgt acggcggtgc
     6481 agccatggtt ggaggtggcg gaaagagaga tataaatcca tggccgccaa cagcaaccat
     6541 gggttttcca cctatgacga caccacccat ggtgccatct cattttagac ctctacacgt
     6601 ctggggtcat ccgcccgccg accaatctat gatgcacatg tggccgaaac atataacgca
     6661 tcttccacca gcatgggcac cggttgttcc tcctcattct tcaccgccta tagatccttc
     6721 cttttggcat tcacaccatc aaagagtact aaactctctt gcaccaggca ctccttgcat
     6781 tccttcacca atagcgccca cgagatttcc agttcagggc atcccaccca ctgccatggt
     6841 caaagctgct cccactggag cacgacaaga tctgcctaaa cctccttctg attttcatcc
     6901 ttcaaaggag agcatagatg cggccattgg agatgtttta gcaaagccat gtctgccacc
     6961 accaccccta ggactcaaac ctccctcaat cgacagtgtg ttgaatgaat tacaacgtca
     7021 agggattaat aaaatacctc caactgaccc agctttcttg tacaaagtgg ttgataacag
     7081 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     7141 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     7201 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     7261 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     7321 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     7381 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     7441 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga
     7501 catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct
     7561 tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg
     7621 tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc
     7681 atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc
     7741 aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga
     7801 aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc
     7861 ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat
     7921 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     7981 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     8041 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     8101 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     8161 tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa
     8221 aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat
     8281 taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga
     8341 ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat
     8401 agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc
     8461 agtattccct caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag
     8521 aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt
     8581 ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg
     8641 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     8701 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     8761 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     8821 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     8881 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     8941 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     9001 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     9061 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     9121 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     9181 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     9241 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     9301 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     9361 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     9421 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     9481 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     9541 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     9601 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt
     9661 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     9721 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     9781 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     9841 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     9901 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     9961 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    10021 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
    10081 ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc ctagaagcta
    10141 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    10201 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    10261 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
    10321 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10381 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
    10441 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10501 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
    10561 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
    10621 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    10681 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
    10741 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    10801 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    10861 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    10921 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    10981 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    11041 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
    11101 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
    11161 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
    11221 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
    11281 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
    11341 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    11401 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    11461 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    11521 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    11581 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    11641 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    11701 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    11761 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    11821 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    11881 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    11941 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    12001 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    12061 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    12121 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    12181 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    12241 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    12301 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    12361 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    12421 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    12481 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    12541 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    12601 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    12661 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    12721 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    12781 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    12841 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    12901 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    12961 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    13021 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    13081 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    13141 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    13201 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    13261 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    13321 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    13381 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    13441 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    13501 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    13561 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    13621 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    13681 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    13741 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    13801 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    13861 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    13921 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    13981 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    14041 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    14101 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    14161 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    14221 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    14281 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    14341 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    14401 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    14461 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    14521 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    14581 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    14641 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    14701 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    14761 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    14821 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    14881 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    14941 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    15001 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    15061 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    15121 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    15181 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    15241 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    15301 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    15361 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    15421 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    15481 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    15541 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    15601 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    15661 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    15721 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    15781 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    15841 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    15901 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    15961 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    16021 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    16081 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    16141 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    16201 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    16261 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    16321 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    16381 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    16441 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    16501 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    16561 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    16621 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    16681 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    16741 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    16801 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    16861 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    16921 gtcggaaagg cgc
//
