LOCUS       sequence-246991-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of a fusion protein encoding SNAP, CLIP and 
            the GB1a subunit of the metabotropic GABA receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Masharina A, Reymond L, Maurel D, Umezawa K, Johnsson K
  TITLE     A fluorescent sensor for GABA and synthetic GABA(B) receptor 
            ligands.
  JOURNAL   J Am Chem Soc. 2012 Nov 21;134(46):19026-34. doi: 10.1021/ja306320s.
            Epub 2012 Nov 5.
  PUBMED    23095089
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja306320s";
            journalName: "J Am Chem Soc"; date: "2012-11-21- 21"; volume: "134";
            issue: "46"; pages: "19026-34"
COMMENT     Sequence Label: pRK5-SNAP-CLIP-GB1a
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1007..1033
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1007..1033
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1046..1588
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             1601..2143
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLAV
                     KEWLLAHEGHRLGKPGLG"
     regulatory      5011..5020
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5024..5158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5061..5080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5115..5134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5227..5584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5227..5418
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5250..5270)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5435..5570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5604..5621)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5604..5620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5613..5635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5833..6288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5920..5939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6130..6151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6305..6324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6424..6446
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6484..6502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6570..6674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6675..6743,6744..7535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6893..6912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7706..8294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8195..8214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8448..8465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8582..8603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8618..8623,8624..8641,8642..8648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8656..8672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8661..8683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8680..8696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8680..8696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcctttcc taaaatggtc cttctgttga tcctgtcagt
      961 cctacttctg aaagaagatg tacgagggag tgcacagtcc acgcgatacc cctacgacgt
     1021 gcccgactac gccctgcagg gatccgacaa agactgcgaa atgaagcgca ccaccctgga
     1081 tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt
     1141 cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct
     1201 gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact ttcaccagcc
     1261 tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag
     1321 ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag
     1381 ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc
     1441 cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacct
     1501 ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg
     1561 ccacagactg ggcaagcctg ggctgggcac gcgtgatatc gacaaagact gcgaaatgaa
     1621 gcgcaccacc ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct
     1681 gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc
     1741 cccagccgcc gtgctgggcg gaccagagcc actgatccag gccaccgcct ggctcaacgc
     1801 ctactttcac cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt
     1861 gttccagcag gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt
     1921 cggagaggtc atcagcgaga gccacctggc cgccctggtg ggcaatcccg ccgccaccgc
     1981 cgccgtgaac accgccctgg acggaaatcc cgtgcccatt ctgatcccct gccaccgggt
     2041 ggtgcagggc gacagcgacg tggggcccta cctgggcggg ctcgccgtga aagagtggct
     2101 gctggcccac gagggccaca gactgggcaa gcctgggctg ggcacgcgtg gcggggcgca
     2161 gacccccaac gccacctcgg aaggttgcca gattatacat ccgccctggg aaggtggcat
     2221 caggtaccgt ggcttgactc gcgaccaggt gaaggccatc aacttcctgc ctgtggacta
     2281 tgagatcgaa tatgtgtgcc gaggggagcg cgaggtggtg gggcccaagg tgcgcaaatg
     2341 cctggccaac ggctcctgga cggatatgga cacacccagc cgctgtgtcc gaatctgctc
     2401 caagtcttat ttgaccctgg aaaatgggaa ggttttcctg acgggtgggg acctcccagc
     2461 tctggatgga gcccgggtgg agttccgatg tgaccccgac ttccatctgg tgggcagctc
     2521 ccggagcgtc tgtagtcagg gccagtggag cacccccaag ccccactgcc aggtgaatcg
     2581 aacgccacac tcagaacggc gtgcagtata catcggggcg ctgtttccca tgagcggggg
     2641 ctggccgggg ggccaggcct gccagcccgc ggtggagatg gcgctggagg acgttaacag
     2701 ccgcagagac atcctgccgg actacgagct caagcttatc caccacgaca gcaagtgtga
     2761 cccagggcaa gccaccaagt acttgtacga actactctac aatgacccca tcaagatcat
     2821 tctcatgcct ggctgtagtt ctgtctccac acttgtagct gaggctgccc ggatgtggaa
     2881 ccttattgtg ctctcatatg gctccagttc accagccttg tcaaaccgac agcggtttcc
     2941 cacgttcttc cggacgcatc catccgccac actccacaat cccacccggg tgaaactctt
     3001 cgaaaagtgg ggctggaaga agatcgctac catccaacag accaccgagg tcttcacctc
     3061 aacgctggat gacctggagg agcgagtgaa agaggctggg atcgagatca ctttccgaca
     3121 gagtttcttc tcggatccag ctgtgcctgt taaaaacctg aagcgtcaag atgctcgaat
     3181 catcgtggga cttttctatg agacggaagc ccggaaagtt ttttgtgagg tctataagga
     3241 aaggctcttt gggaagaagt acgtctggtt cctcatcggg tggtatgctg acaactggtt
     3301 caagacctat gacccgtcaa tcaattgtac agtggaagaa atgaccgagg cggtggaggg
     3361 ccacatcacc acggagattg tcatgctgaa ccctgccaac acccgaagca tttccaacat
     3421 gacgtcacag gaatttgtgg agaaactaac caagcggctg aaaagacacc ccgaggagac
     3481 tggaggcttc caggaggcac cactggccta tgatgctatc tgggccttgg ctttggcctt
     3541 gaacaagacg tctggaggag gtggtcgttc cggcgtgcgc ctggaggact ttaactacaa
     3601 caaccagacc attacagacc agatctaccg ggccatgaac tcctcctcct ttgagggcgt
     3661 ttctggccat gtggtctttg atgccagcgg ctcccggatg gcatggacac ttatcgagca
     3721 gctacagggc ggcagctaca agaagatcgg ctactacgac agcaccaagg atgatctttc
     3781 ctggtccaaa acggacaagt ggattggagg gtctccccca gctgaccaga ccttggtcat
     3841 caagacattc cgtttcctgt ctcagaaact ctttatctcc gtctcagttc tctccagcct
     3901 gggcattgtt cttgctgttg tctgtctgtc ctttaacatc tacaactccc acgttcgtta
     3961 tatccagaac tcccagccca acctgaacaa tctgactgct gtgggctgct cactggcact
     4021 ggctgctgtc ttccctctcg ggctggatgg ttaccacata gggagaagcc agttcccgtt
     4081 tgtctgccag gcccgccttt ggctcttggg cttgggcttt agtctgggct atggctctat
     4141 gttcaccaag atctggtggg tccacacagt cttcacgaag aaggaggaga agaaggagtg
     4201 gaggaagacc ctagagccct ggaaactcta tgccactgtg ggcctgctgg tgggcatgga
     4261 tgtcctgact cttgccatct ggcagattgt ggaccccttg caccgaacca ttgagacttt
     4321 tgccaaggag gaaccaaagg aagacatcga tgtctccatt ctgccccagt tggagcactg
     4381 cagctccaag aagatgaata cgtggcttgg cattttctat ggttacaagg ggctgctgct
     4441 gctgctggga atctttcttg cttacgaaac caagagcgtg tccactgaaa agatcaatga
     4501 ccacagggcc gtgggcatgg ctatctacaa tgtcgcggtc ctgtgtctca tcactgctcc
     4561 tgtgaccatg atcctttcca gtcagcagga cgcagccttt gcctttgcct ctctggccat
     4621 cgtgttctct tcctacatca ctctggttgt gctctttgtg cccaagatgc gcaggctgat
     4681 cacccgaggg gaatggcagt ctgaaacgca ggacaccatg aaaacaggat catccaccaa
     4741 caacaacgag gaagagaagt cccgactgtt ggagaaggaa aaccgagaac tggaaaagat
     4801 catcgctgag aaagaggagc gcgtctctga actgcgccat cagctccagt ctcggcagca
     4861 actccgctca cggcgccacc ccccaacacc cccagatccc tccgggggcc ttcccagggg
     4921 accctctgag ccccctgacc ggcttagctg tgatgggagt cgagtacatt tgctttacaa
     4981 gtgattctag agtcgacctg cagaagcttg gccgccatgg cccaacttgt ttattgcagc
     5041 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5101 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgg
     5161 gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     5221 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5581 caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5641 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5701 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5761 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5821 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5881 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5941 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6001 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6061 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6121 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     6181 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6241 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6301 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6361 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6421 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6481 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6541 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8581 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8641 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     8701 ttacgaatta a
//
