LOCUS       sequence-247102-       16418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16418)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX109
FEATURES             Location/Qualifiers
     source          1..16418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6532..6556)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6585..6674
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6708..6960
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7389..7641)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8109..9134)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8109..9134)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9144..9488)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9630..9647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9630..9646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9639..9661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9786..9805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9996..10017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10264..10288
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11884..13032)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13331..14125)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14317..15084
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15777..16408
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtgtttg cagtaatgag ttgcaacggt tgtcgaattc
     5881 tacggaaagg ctgcaacgag aattgcatac tgcgacaaag tttacaaggc attgaaagcc
     5941 ctcatgctca agcaaatgcc accgtctttg tggccaaatt ctttggccgt gctggcctca
     6001 tgtctttcct ctcctctgtt cccgaatctc aacgtccagc tctattccaa tcgctgcttt
     6061 tcgaatcgtg tggaaggacg gtgaacccgg tgcacggagc ggtcggatta ttgtggacgg
     6121 gaaactggca cgtgtgccag tcagcggtag aaacggtgct taaaggaggc gtgttacggc
     6181 cgttgccgga gttttcaggt gtaacagctt taccggagtc cgattcagca tccaaggcga
     6241 acgatgttaa tctctttaga tcgcaaagct tcaacttccg atctaagagg aaagttatgg
     6301 acgaggtggc ggaggatcta gatctcggat tatcgtcagg attcccggtg aaaatgacgg
     6361 ccacgggtgg tagaatgaac cggcggacgg agaagagacg agaggctaca ccttcgttga
     6421 attctgatga atctgatacc acaacactag aaagtggttt tatgtgccat caaaatccgc
     6481 aacaaggaca aagagatgat actaaacttc ttagattgtt ctttcccggg gacccagctt
     6541 tcttgtacaa agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc
     6601 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
     6661 ttccagatta cgctgctcag tgcagcgctt agagctcgaa tttccccgat cgttcaaaca
     6721 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     6781 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     6841 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     6901 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     6961 gggaattagc ttcatcaacg caagacatgc gcacgaccgt ctgacaggag aggaatttcc
     7021 gacgagcaca gaaaggactt gctcttggac gtaggcctat ttctcaggca catgtatcaa
     7081 gtgttcggac gtgggttttc gatggtgtat cagccgccgc caactgggag atgaggaggc
     7141 tttcttgggg ggcagtcagc agttcatttc acaagacaga ggaacttgta aggagatgca
     7201 ctgatttatc ttggcgcaaa ccagcaggac gaattagtgg gaatagcccg cgaatatcta
     7261 agttatgcct gtcggcatga gcagaaactt ccaattcgaa acagtttgga gaggttgttt
     7321 ttgggcatac cttttgttag tcagcctctc gattgctcat cgtcattaca cagtaccgaa
     7381 gtttgatcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
     7441 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
     7501 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
     7561 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
     7621 ttgccaaatg tttgaacgat ctgcttcgac gcactccttc tttactccac catctcgtcc
     7681 ttattgaaaa cgtgggtagc accaaaacga atcaagtcgc tggaactgaa gttaccaatc
     7741 acgctggatg atttgccagt tggattaatc ttgcctttcc ccgcatgaat aatattgatg
     7801 aatgcatgcg tgaggggtat ttcgattttg gcaatagctg caattgccgc gacatcctcc
     7861 aacgagcata attcttcaga aaaatagcga tgttccatgt tgtcagggca tgcatgatgc
     7921 acgttatgag gtgacggtgc taggcagtat tccctcaaag tttcatagtc agtatcatat
     7981 tcatcattgc attcctgcaa gagagaattg agacgcaatc cacacgctgc ggcaaccttc
     8041 cggcgttcgt ggtctatttg ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg
     8101 catctactct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     8161 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     8221 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     8281 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     8341 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     8401 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     8461 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     8521 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     8581 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     8641 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     8701 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     8761 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     8821 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
     8881 tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     8941 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     9001 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     9061 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     9121 tcaggctttt tcatatcggg gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg
     9181 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     9241 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     9301 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     9361 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     9421 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     9481 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     9541 gagtgtcgtg ctccaccatg ttgacggatc tctaggacgc gtcgacgcgt cgacgcgtcg
     9601 acgcgtcgac gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg
     9661 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9721 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9781 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9841 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9901 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9961 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10021 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga
    10081 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
    10141 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
    10201 aaaaccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    10261 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    10321 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    10381 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    10441 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    10501 tgacagagcg ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca
    10561 gccttcttat tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga
    10621 cataatagga aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga
    10681 agtgaacgtt gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg
    10741 acccgaagtt ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc
    10801 tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa
    10861 ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc
    10921 ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc
    10981 cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg
    11041 tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag
    11101 gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag
    11161 cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga
    11221 ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg
    11281 aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca
    11341 tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca
    11401 acgcccgcag cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc
    11461 cggacagatg cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc
    11521 caatgatctc gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga
    11581 acgcctccat gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt
    11641 tcttcagggc cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc
    11701 gaatctgagc cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga
    11761 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
    11821 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
    11881 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
    11941 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
    12001 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    12061 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    12121 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    12181 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    12241 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    12301 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    12361 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    12421 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    12481 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    12541 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    12601 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    12661 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    12721 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    12781 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    12841 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    12901 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    12961 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    13021 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    13081 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    13141 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    13201 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
    13261 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
    13321 catctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    13381 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    13441 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    13501 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    13561 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    13621 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    13681 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    13741 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    13801 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    13861 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    13921 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    13981 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    14041 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    14101 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    14161 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    14221 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    14281 agtatcgacg gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt
    14341 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
    14401 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
    14461 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
    14521 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc
    14581 ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag
    14641 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
    14701 cagtggggct atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt
    14761 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
    14821 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
    14881 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt
    14941 gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg
    15001 ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
    15061 tatcaataag ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg
    15121 tcctgggttt caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata
    15181 ttgataagcg cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct
    15241 atataaaaga tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc
    15301 atcctcttca tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc
    15361 tgtaaaggtc caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg
    15421 ttcgctgggt ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga
    15481 atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa
    15541 gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat
    15601 gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc
    15661 catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg
    15721 gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg
    15781 ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag
    15841 ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac
    15901 aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt
    15961 ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag
    16021 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag
    16081 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt
    16141 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc
    16201 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct
    16261 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc
    16321 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc
    16381 ggcgagggca accagcccgg tgagcgtcgg aaaggcgc
//
