LOCUS       sequence-247104-       14974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14974)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX110
FEATURES             Location/Qualifiers
     source          1..14974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5088..5112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5141..5230
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5264..5516
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5945..6197)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6665..7690)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6665..7690)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7700..8044)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8186..8203)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8186..8202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8195..8217)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8342..8361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8552..8573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8820..8844
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10440..11588)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11887..12681)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12873..13640
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14333..14964
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg agcagtggac cgtgtggtgc
     4621 atgcaaatac cttagaagaa agtgtgtaaa gggttgcata tttgcacctt attttgatac
     4681 agaacaaggc atgtctcact ttgctgctgt ccacaaggtg tttggagcaa gcaatgcctc
     4741 caaattattg cttagtattc ctgcaaacaa gcgtcccgaa actgttatta ccctctgcta
     4801 tgaggctctc gctagagtta gagaccctgt ttatggctct gttgctcaca ttttcactct
     4861 tcaacaacag gttttgacat tgcaagcaga gttaggttat gttcaagccc gtctttccac
     4921 cctacaatct gcttcttcag aacaggcagc aacttattct aacatttcat cgctgtctat
     4981 ggatactttg ttcgatacaa caggatttca ttatgtgtta gatgaagaag tggagaattg
     5041 tgacatacat tctgtagctg tagatttcgt ctctagacgc cccggggacc cagctttctt
     5101 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     5161 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     5221 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     5281 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     5341 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     5401 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     5461 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     5521 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     5581 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     5641 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     5701 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     5761 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     5821 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     5881 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     5941 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6001 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     6061 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     6121 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     6181 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     6241 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     6301 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     6361 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     6421 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     6481 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     6541 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     6601 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     6661 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     6721 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     6781 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     6841 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     6901 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     6961 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     7021 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     7081 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     7141 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     7201 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     7261 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     7321 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     7381 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     7441 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     7501 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     7561 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     7621 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     7681 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     7741 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     7801 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     7861 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     7921 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     7981 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     8041 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     8101 gtcgtgctcc accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc
     8161 gtcgacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8221 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8281 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc
     8341 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8401 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8461 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8521 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8581 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca
     8641 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     8701 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     8761 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     8821 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     8881 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     8941 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     9001 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     9061 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
     9121 tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata
     9181 ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg
     9241 aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc
     9301 gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag
     9361 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
     9421 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
     9481 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
     9541 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
     9601 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
     9661 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
     9721 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
     9781 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
     9841 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
     9901 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
     9961 ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga
    10021 cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat
    10081 gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc
    10141 ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt
    10201 cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat
    10261 ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc
    10321 gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc
    10381 tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    10441 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    10501 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    10561 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    10621 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    10681 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    10741 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    10801 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    10861 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    10921 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    10981 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    11041 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    11101 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    11161 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    11221 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    11281 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    11341 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    11401 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    11461 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    11521 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    11581 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
    11641 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
    11701 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
    11761 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
    11821 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
    11881 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    11941 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    12001 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    12061 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    12121 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    12181 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    12241 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    12301 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    12361 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    12421 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    12481 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    12541 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    12601 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    12661 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    12721 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    12781 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    12841 tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt
    12901 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    12961 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
    13021 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
    13081 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
    13141 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
    13201 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
    13261 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
    13321 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
    13381 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
    13441 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
    13501 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
    13561 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
    13621 aataagttgg agtcattacc caattatgat agaatttaca agctataagg ttattgtcct
    13681 gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga
    13741 taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat
    13801 aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc
    13861 tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta
    13921 aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg
    13981 ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc
    14041 ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga
    14101 agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg
    14161 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
    14221 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga
    14281 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    14341 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    14401 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    14461 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    14521 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    14581 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    14641 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    14701 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    14761 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    14821 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
    14881 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
    14941 agggcaacca gcccggtgag cgtcggaaag gcgc
//
