LOCUS       sequence-247293-       15292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15292)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX122
FEATURES             Location/Qualifiers
     source          1..15292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5406..5430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5459..5548
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5582..5834
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6263..6515)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6983..8008)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6983..8008)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8018..8362)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8504..8521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8504..8520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8513..8535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8660..8679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8870..8891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9138..9162
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10758..11906)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12205..12999)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13191..13958
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14651..15282
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtgtag
     4621 cagagggcat tggagacctc atgaagatga gaaactccgg gagttagtcg ctaaatatgg
     4681 acctcataat tggaatgcta ttgctgaaaa cttgcaaggc agatcaggga agagttgtag
     4741 gttgagatgg tacaatcagt tggatccaag aataaacaga agcccattca cagaagagga
     4801 agaagaaaga ctacttgcct ctcacagaat tcatgggaat agatgggcta taattgctag
     4861 gctttttcca ggtcgtactg ataatgctgt taaaaatcat tggcacgtta ttatgtcaag
     4921 aagatgcaga gaaagatcta agatttattc tatgagaaat attgctgctg cccctaaatc
     4981 aacaagccaa caagacatga atacgacttc accgagtgat catctgcatg atgataataa
     5041 tatccacaat atcatgagaa aaagaagttc caatatttct attgaccatc atcaccaatt
     5101 attagaaaga tttaattatt atcctttcac taataataat tccctttatc caaaagagct
     5161 tcatttcaat caccttccag ctcatcattt gaagatggat gaaggcaaga ataatgaagc
     5221 agtggaatgt tatgactttc tacgagtgaa tacaggctca aataaaagtg aagttgtaga
     5281 ggataatatt acaagaaggg atgctgagga agtggaacaa gaagctgggg ataataattc
     5341 cctacaaagc aaggctccag tgcgctttat agatttcctc tcagttggag attcatcgcc
     5401 cggggaccca gctttcttgt acaaagtggt tgataacagc gggttaatta acatctttta
     5461 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc
     5521 ctatccatat gacgttccag attacgctgc tcagtgcagc gcttagagct cgaatttccc
     5581 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     5641 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     5701 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     5761 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     5821 tatgttacta gatcgggaat tagcttcatc aacgcaagac atgcgcacga ccgtctgaca
     5881 ggagaggaat ttccgacgag cacagaaagg acttgctctt ggacgtaggc ctatttctca
     5941 ggcacatgta tcaagtgttc ggacgtgggt tttcgatggt gtatcagccg ccgccaactg
     6001 ggagatgagg aggctttctt ggggggcagt cagcagttca tttcacaaga cagaggaact
     6061 tgtaaggaga tgcactgatt tatcttggcg caaaccagca ggacgaatta gtgggaatag
     6121 cccgcgaata tctaagttat gcctgtcggc atgagcagaa acttccaatt cgaaacagtt
     6181 tggagaggtt gtttttgggc ataccttttg ttagtcagcc tctcgattgc tcatcgtcat
     6241 tacacagtac cgaagtttga tcgatctagt aacatagatg acaccgcgcg cgataattta
     6301 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     6361 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     6421 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     6481 cttaagaaac tttattgcca aatgtttgaa cgatctgctt cgacgcactc cttctttact
     6541 ccaccatctc gtccttattg aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac
     6601 tgaagttacc aatcacgctg gatgatttgc cagttggatt aatcttgcct ttccccgcat
     6661 gaataatatt gatgaatgca tgcgtgaggg gtatttcgat tttggcaata gctgcaattg
     6721 ccgcgacatc ctccaacgag cataattctt cagaaaaata gcgatgttcc atgttgtcag
     6781 ggcatgcatg atgcacgtta tgaggtgacg gtgctaggca gtattccctc aaagtttcat
     6841 agtcagtatc atattcatca ttgcattcct gcaagagaga attgagacgc aatccacacg
     6901 ctgcggcaac cttccggcgt tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt
     6961 tggatcccgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt
     7021 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     7081 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     7141 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     7201 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     7261 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     7321 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     7381 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     7441 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     7501 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     7561 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     7621 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     7681 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     7741 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     7801 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     7861 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     7921 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     7981 acgtcgcggt gagttcaggc tttttcatat cggggtcgtc ctctccaaat gaaatgaact
     8041 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     8101 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     8161 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     8221 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     8281 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     8341 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     8401 cttggagtag acgagagtgt cgtgctccac catgttgacg gatctctagg acgcgtcgac
     8461 gcgtcgacgc gtcgacgcgt cgacgcgtcc tagaagctaa ttcactggcc gtcgttttac
     8521 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8641 gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8701 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8761 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     8821 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8881 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     8941 ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     9001 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     9061 cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt gttattaagt
     9121 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     9181 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     9241 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     9301 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     9361 gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga
     9421 gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt
     9481 gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc
     9541 gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg ctcaccgaat
     9601 ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg gctgatcgac
     9661 cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     9721 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     9781 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     9841 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     9901 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     9961 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
    10021 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
    10081 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
    10141 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
    10201 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
    10261 taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg
    10321 ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt
    10381 gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg
    10441 caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa
    10501 catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc
    10561 cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat
    10621 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
    10681 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
    10741 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
    10801 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
    10861 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
    10921 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
    10981 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
    11041 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
    11101 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
    11161 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
    11221 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
    11281 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
    11341 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
    11401 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
    11461 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
    11521 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
    11581 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
    11641 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
    11701 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
    11761 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
    11821 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
    11881 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
    11941 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
    12001 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
    12061 ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc
    12121 tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg
    12181 gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    12241 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    12301 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    12361 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    12421 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    12481 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    12541 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    12601 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    12661 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    12721 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    12781 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    12841 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    12901 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    12961 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    13021 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    13081 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    13141 ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg
    13201 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
    13261 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
    13321 ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac
    13381 accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
    13441 tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
    13501 aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg
    13561 cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt
    13621 acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg
    13681 cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg
    13741 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat
    13801 atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac
    13861 ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc
    13921 tgaacataaa acactatcaa taagttggag tcattaccca attatgatag aatttacaag
    13981 ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc
    14041 attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata
    14101 cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg
    14161 caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct
    14221 tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct
    14281 atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg gcacccgcga
    14341 ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg
    14401 ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca ctgcccggca
    14461 cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc
    14521 tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg
    14581 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
    14641 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
    14701 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
    14761 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
    14821 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
    14881 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
    14941 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
    15001 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
    15061 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
    15121 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
    15181 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    15241 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gc
//
