LOCUS       sequence-247350-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0629
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(12..116)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      219..807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     708..727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1825..1845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2128..3621
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(join(4147..4938,4939..4996,1..11))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4770..4789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
       61 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      121 tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt ttcgttccac
      181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      841 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      901 aaccgtgcgg ccgcccctga attcgcatct agaacggggt catcacggct catcatgcgc
      961 caaacaaatg tgtgcaatac acgctcggat gactgcatga tgaccgcact gactggggac
     1021 agcagatcca cctaagcctg tgagagaagc agacacccga cagatcaagg cagttacgtc
     1081 tcaccaaacc agatgtcaac acagctacaa cgagtcgaac aagaagcagg caaagtttag
     1141 agcactgccc ctccgcactc aaaaaagaaa aaactaggag gaaaataaaa ttctcaacca
     1201 cacaaacaca taaacacata caaatacaaa tacaagctta tttacttgac atcgcgcgat
     1261 cttccactat tcagcgccgt ccgccctctc tcgtgttttt tgtttacgcg acaactatgc
     1321 gaaatccgga gcaacgggca accgtttggg gaaagaccac acccacgcgc gatcgccatg
     1381 gcaacgaggt cgcacacgcc ccacacccag acctccctgc gagcgggcat gggtacaatg
     1441 tccccgttgc cacagacacc acttcgtagc acagcgcaga gcgtagcgtg ttgttgctgc
     1501 tgacaaaaga aaatttttct tagcaaagca aaggagggga agcacgggca gatagcaccg
     1561 taccataccc ttggaaactc gaaatgaacg aagcaggaaa tgagagaatg agagttttgt
     1621 aggtatatat agcggtagtg tttgcgcgtt accatcatct tctggatcta tctattgttc
     1681 ttttcctcat cactttcccc tttttcgctc ttcttcttgt cttttatttc tttctttttt
     1741 ttaattgttc cctcgattgg ctatctacca aagaatccaa acttaataca cgtatttatt
     1801 tgtccaatta ccagatctat ggcccccaag aagaagagga aggttggtat tcacgggggt
     1861 acccgcccat atgcttgccc tgtcgagtcc tgcgatcgcc gcttttctcg ctcggatgag
     1921 cttacccgcc atatccgcat ccataccggt cagaagccct tccagtgtcg aatctgcatg
     1981 cgtaacttca gtcgtagtga ccaccttacc acccacatcc gcacccacac aggcgagaag
     2041 ccttttgcct gtgacatttg tgggaggaag tttgccagga gtgatgaacg caagaggcat
     2101 accaaaatcc atacaggtgc tagcttcatg aagatggaca aaaagactat agtttggttt
     2161 agaagagatc taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct
     2221 gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct
     2281 tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga
     2341 tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc
     2401 gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg
     2461 gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga
     2521 gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc
     2581 aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct
     2641 tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa
     2701 ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggagt
     2761 ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat
     2821 tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc
     2881 catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata
     2941 tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc
     3001 ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg
     3061 ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg
     3121 agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc
     3181 ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc
     3241 cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa
     3301 tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat
     3361 cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg
     3421 caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct
     3481 cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt
     3541 gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca
     3601 cagatcatga tcggagcagc agcccgggga tcctaactcg aggccaattg gtgcggcaat
     3661 tgataataac gaaaatgtct tttaatgatc tgggtataat gaggaatttt ccgaacgttt
     3721 ttactttata tatatatata catgtaacat atattctata cgctatagag aaaggaaatt
     3781 tttcaattaa aaaaaaaata gagaaagagt ttcacttctt gattatcgct aacactaatg
     3841 gttgaagtac tgctacttta attttatgct ggatgtgaga cgataaatcg cagccaagtg
     3901 agtgaataga tgacgcacca cggtcagaca cggccacatc gtatctcaca ggagcaagcg
     3961 cgataggagc actcacacat agtacggtga tccgctgact cctttgccca aataagacgt
     4021 gagccactag tgcactgcag tacagcggcc gcgattatca aaaaggatct tcacctagat
     4081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4261 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     4321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4981 ggcgacacgg aaatgt
//
