LOCUS       sequence-247353-       11260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable 
            KIURA3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0646
FEATURES             Location/Qualifiers
     source          1..11260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1891..1911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2194..3687
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     4618..4641
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4665..5375
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      5391..5578
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5446..5470)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    5684..5717
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7170..7203
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(7787..8602)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7787..8602)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8510..8529
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8594..8613
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8827..9415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9316..9335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10368..10781
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             10831..10851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     11079..11098
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11079..11097
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(11122..11260,1..371)
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 ttaccggcgg cgagatggac agctatcttt cgactgccgg tttgaatctt ccgatgatgt
       61 acggtgaaac gacggtggaa ggtgattcaa gactctcaat ttcgccggaa acgacgcttg
      121 ggactggaaa tttcaagaaa cggaagtttg atacagagac taaggattgt aatgagaaga
      181 agaagaagat gacgatgaac agagatgacc tagtagaaga aggagaagaa gagaagtcga
      241 aaataacaga gcaaaacaat gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca
      301 agaaagaaga gaacaatttc tctaatgatt catctaaagt gacgaaggaa ttggagaaaa
      361 cggattatat tcatgttcgt gcacgacgag gccaagccac tgatagtcac agcatagcag
      421 aacgagttag aagagaaaag atcagtgaga gaatgaagtt tctacaagat ttggttcctg
      481 gatgcgacaa gatcacaggc aaagcaggga tgcttgatga aatcattaac tatgttcagt
      541 ctcttcagag acaaatcgag ttcttatcga tgaaactagc aattgtgaat ccaaggccgg
      601 attttgatat ggatgacatt tttgccaaag aggttgcctc aactccaatg actgtggtgc
      661 catctcctga aatggttctt tccggttatt ctcatgagat ggttcactct ggttattcta
      721 gtgagatggt taactccggt taccttcatg tcaatccaat gcagcaagtg aataccagtt
      781 ctgatccatt gtcatgcttc aacaatggcg aagctccttc gatgtgggac tctcatgtgc
      841 agaatctcta tggcaattta ggagtaccgg tcatcgagct cgagctgcag atgaatcgta
      901 gatacggatc ctaactcgag agcttttgat taagccttct agtccaaaaa acacgttttt
      961 ttgtcattta tttcattttc ttagaatagt ttagtttatt cattttatag tcacgaatgt
     1021 tttatgattc tatatagggt tgcaaacaag catttttcat tttatgttaa aacaatttca
     1081 ggtttacctt ttattctgct tgtggtgacg cgtgtatccg cccgctcttt tggtcaccca
     1141 tgtatgctgc caaaccagat gtcaacacag ctacaacgaa gaggatgtcc aatatttttt
     1201 ttaaggaata aggatacttc aagactagat tcccccctgc attcccatca gaaccgtaaa
     1261 ccttggcgct ttccttggga agtattcaag aagtgccttg tccggtttct gtggctcaca
     1321 aaccagcgcg cccgatatgg ctttcttttc acttatgaat gtaccagtac gggacaatta
     1381 gaacgctcct gtaacaatct ctttgcaaat gtggggttac attctaacca tgtcacactg
     1441 ctgacgaaat tcaaagtaaa aaaaaatggg accacgtctt gagaacgata gattttcttt
     1501 attttacatt gaacagtcgt tgtctcagcg cgctttatgt tttcattcat acttcatatt
     1561 ataaaataac aaaagaagaa tttcatattc acgcccaaga aatcaggctg ctttccaaat
     1621 gcaattgaca cttcattagc catcacacaa aactctttct tgctggagct tcttttaaaa
     1681 aagacctcag tacaccaaac acgttacccg acctcgttat tttacgacaa ctatgataaa
     1741 attctgaaga aaaaataaaa aaattttcat acttcttgct tttatttaaa ccattgaatg
     1801 atttcttttg aacaaaacta cctgtttcac caaaggaaat agaaagaaaa aatcaattag
     1861 aagaaaacaa aaaacaaaag atctatggcc cccaagaaga agaggaaggt tggtattcac
     1921 gggggtaccc gcccatatgc ttgccctgtc gagtcctgcg atcgccgctt ttctcgctcg
     1981 gatgagctta cccgccatat ccgcatccat accggtcaga agcccttcca gtgtcgaatc
     2041 tgcatgcgta acttcagtcg tagtgaccac cttaccaccc acatccgcac ccacacaggc
     2101 gagaagcctt ttgcctgtga catttgtggg aggaagtttg ccaggagtga tgaacgcaag
     2161 aggcatacca aaatccatac aggtgctagc ttcatgaaga tggacaaaaa gactatagtt
     2221 tggtttagaa gagatctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa
     2281 ggatctgttt ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga
     2341 agagcttcaa gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct
     2401 cttggatctg acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt
     2461 atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta
     2521 gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac
     2581 aatggagatc tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg
     2641 agtttcaatt cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct
     2701 cctccttggc ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt
     2761 gaagaactag ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct
     2821 tggagtccag gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg
     2881 atagattatg caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg
     2941 tatctccatt tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa
     3001 attatatggg caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg
     3061 ggaatcggtt taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag
     3121 caatcgttgt tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag
     3181 gcctggagac aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg
     3241 gctaccggat ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt
     3301 cttctccttc catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat
     3361 ttggaatgtg acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag
     3421 cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac
     3481 ataaggcaat ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg
     3541 gacgctcctt taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa
     3601 cccattgtag acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt
     3661 gaagcacaga tcatgatcgg agcagcagcc cggggatcct aactcgaggc caattggtgc
     3721 ggcaattgat aataacgaaa atgtctttta atgatctggg tataatgagg aattttccga
     3781 acgtttttac tttatatata tatatacatg taacatatat tctatacgct atagagaaag
     3841 gaaatttttc aattaaaaaa aaaatagaga aagagtttca cttcttgatt atcgctaaca
     3901 ctaatggttg aagtactgct actttaattt tatgctggat gcacacactg gcttaagatg
     3961 acaacgcgag gcaagctaaa cagatcttta tattgaattt tcaaaaattc ttactttttt
     4021 tttggatgga cgcaaagaag tttaataatc atattacatg gcattaccac catatacata
     4081 tccatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat
     4141 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt
     4201 gctatattga agtgcgcgcg cgcgtgggcg agcgtgggcg agcgtgggcg tctagaccgt
     4261 gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct
     4321 ccgaacaata aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag
     4381 taacctggcc ccacaaacct tcaaattaac gaatcaaatt aacaaccata ggatgataat
     4441 gcgattagtt ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat
     4501 ctattaacag atatataaat ggaaaagctg cataaccact ttaactaata ctttcaacat
     4561 tttcagtttg tattacttct tattcaaatg tcataaaagt atcaacaaaa aattgttaat
     4621 atacctctat actttaacgt caaggagaaa aaactatagg tactatggtg tccaaaggag
     4681 aggagttaat caaggaaaac atgagaatga aagttgtcat ggagggctcc gttaatggtc
     4741 accaattcaa gtgtacaggg gaaggtgaag gtaatcctta catgggtaca caaactatga
     4801 gaattaaagt aattgaaggc ggaccactac catttgcatt tgacattctg gcaacgtcat
     4861 tcatgtacgg atcacgaact ttcatcaagt accctaaagg tataccagac tttttcaagc
     4921 aatcttttcc agagggtttt acatgggaaa gggttacaag atacgaagat gggggtgtcg
     4981 tcacagttat gcaagatact tcattagaag atggctgcct tgtctatcat gtgcaagtaa
     5041 gaggggtgaa ttttccttct aacggacctg tgatgcagaa aaagaccaaa ggttgggaac
     5101 caaatactga aatgatgtac ccagctgatg gaggtttgag aggctacaca cacatggcgc
     5161 ttaaagttga tggtggaggt catttgtctt gtagttttgt taccacttat cgttctaaaa
     5221 agactgttgg caatatcaaa atgccaggaa tacatgctgt agaccacaga ctagaaagac
     5281 tcgaagagag cgataacgaa atgttcgttg tacagagaga gcatgccgta gccaaatttg
     5341 ctggcttagg cggtggtatg gatgaattgt ataagggatc ctaactcgag gcgaatttct
     5401 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     5461 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     5521 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     5581 tgccgagcaa atgcctgcaa atcgctcccc atttcgctga gcagttacag agatgttacg
     5641 aaccactagt gcactgcagt acagcaggtc gacaaccctt aatataactt cgtataatgt
     5701 atgctatacg aagttattag gtctagagat cccaatacaa cagatcacgt gatcttttgt
     5761 aagatgaagt tgaagtgagt gttgcaccgt gccaatgcag gtggctatta gattaaatat
     5821 gtgatttgtt ctattaagtt tcctgtataa ttaatgggga gcgctgattc tcttttggta
     5881 cgcttcccat ccagcatttc tgtatctttc accttcaacc ttaggatctc tacccttggc
     5941 gaaaagtcct ctgccaacaa tgatgatatc tgatccacca cttacaactt cgtcgacggt
     6001 tctgtactgc tgacccaatg catcgccttt gtcgtctaaa cctacacctg gggtcatgat
     6061 tagccaatca aacccttctt ctcttcctcc catatcgttc tgagcaatga acccaataac
     6121 gaaatcttta tcactctttg caatatcaac ggtaccctta gtatattcac cgtgtgctag
     6181 agaacccttg gaagacaatt cagcaagcat caataatccc cttggttctt tggtgacctc
     6241 ttgcgcacct tgtttcaagc cagcaacaat accagcacca gtaaccccgt gggcgttggt
     6301 gatatcagac cattctgcga tacggtaaac gcccgatgta tattgtaatt tgactgtgtt
     6361 accgatatcg gcgaattttc tgtcctcaaa tatcaagaac ttgtatttct ctgccaatgc
     6421 tttcaatgga acgacagtac cctcataact gaaatcatcc aagatatcaa cgtgtgtttt
     6481 caaaaggcaa atgtatggac ccaacgtttc aacaagtttc aatagctcat cagtcgaacg
     6541 aacgtcaaga gaagcacaca aattggtctt cttttcatcc attaaacgta aaagtttcga
     6601 tgcaaccgga cttgcatgag tctcagctct actggtatat gattttgtgg acatggtgca
     6661 actaattgac gggagtgtat tgacgctggc gtactggctt tcacaaaatg gcccaatcac
     6721 aaccacatct tagatagttg aaatgacttt agataacatc aattgagatg agcttaatca
     6781 tgtcaaagct aaaagtgtca ccatgaacga caattcttaa gcaaatcacg tgatatagat
     6841 ccacgaataa ccaccatttg atgctcgagg caagtaatgt gtgtaaaaaa atgcgttacc
     6901 accatccaat gcagaccgat cttctaccca gaatcacata tatttatgta ccgagtacct
     6961 tttttctatc ttccaattgc ttctcccata tgattgtctc cgtaagctcg aaatttctaa
     7021 gttggatttt aatcttcacg caggatgaca gttcgatgag cttctgagga gtgtttagaa
     7081 cataatcagt ttatccatgg tctatctctt cttgtcgctt tttctcctcg atagaaccta
     7141 aataaaacga gctctcgaga acccttaata taacttcgta taatgtatgc tatacgaagt
     7201 tattaggtga tatcagatcc actgagtaga gcacttgaat ccactgcccc gggaatctcg
     7261 gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg aaagaaaaag cttcatggcc
     7321 tttataaaaa ggaactatcc aatacctcgc cagaaccaag taacagtatt ttacggggca
     7381 caaatcaaga acaataagac aggactgtaa agatggacgc attgaactcc aaagaacaac
     7441 aagagttcca aaaagtagtg gaacaaaagc aaatgaagga tttcatgcgt ttgtactcta
     7501 atctggtaga aagatgtttc acagactgtg tcaatgactt cacaacatca aagctaacca
     7561 ataaggaaca aacatgcatc atgaagtgct cagaaaagtt cttgaagcat agcgaacgtg
     7621 tagggcagcg tttccaagaa caaaacgctg ccttgggaca aggcttgggc cgataaggtg
     7681 tactggcgta tatatatcta attatgtatc tctggtgtag cccattttta gcatgtaaat
     7741 ataaagaccg agcggccgcg tgttacaacc aattaaccaa ttctgattag aaaaactcat
     7801 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     7861 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     7921 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     7981 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     8041 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     8101 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc
     8161 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     8221 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     8281 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     8341 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     8401 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     8461 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     8521 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg
     8581 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     8641 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
     8701 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa
     8761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9481 tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gccaatgcta aattcgagtg
     9541 aaacacagga agatcagaaa atcctcattt catccatatt aacaataatt tcaaatgttt
     9601 atttgcatta tttgaaacta ggcaagacaa gcaacgaaac gtttttgaaa attttgagta
     9661 ttttcaataa atttgtagag gactcagata ttgaaaaaaa gctacagcaa ttaatacttg
     9721 ataagaagag tattgagaag ggcaacggtt catcatctca tggatctgca catgaacaaa
     9781 caccagagtc aaacgacgtt gaaattgagg ctactgcgcc aattgatgac aatacagacg
     9841 atgataacaa accgaagtta tctgatgtag aaaaggatta aagatgctaa gagatagtga
     9901 tgatatttca taaataatgt aattctatat atgttaatta ccttttttgc gaggcatatt
     9961 tatggtgaag gataagtttt gaccatcaaa gaaggttaat gtggctgtgg tttcagggtc
    10021 cataaagccc acatggataa cattacccct gaattcgcat ctagactgaa ctggccgata
    10081 attgcagacg aacgccttgc caacagggag ttcttcagag acatggaggc tcaaaacgaa
    10141 attattgaca gcctagacat caatagtcat acaacagaaa gcgaccaccc aactttggct
    10201 gataatagcg tataaacaat gcatactttg tacgttcaaa atacaatgca gtagatatat
    10261 ttatgcatat tacatataat acatatcaca taggaagcaa caggcgcgtt ggacttttaa
    10321 ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat cccccacaca
    10381 ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg
    10441 catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc
    10501 tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg
    10561 tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa
    10621 attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
    10681 cggtcatcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
    10741 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaaagatct
    10801 atggataaag cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt
    10861 accgccgcct cggagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg
    10921 ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc
    10981 ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg
    11041 tttaccgatg cccttggaat tgacgagtac ggtgggttta atacgactca ctatagggcg
    11101 agcgccgaag ctagcgccac catgaatgga gctataggag gtgacctttt gctcaatttt
    11161 cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt
    11221 gattctcctc tcgccggctt ctttgccgat tcttcaatga
//
