LOCUS       sequence-247452-       15595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15595)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX127
FEATURES             Location/Qualifiers
     source          1..15595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5709..5733)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5762..5851
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5885..6137
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6566..6818)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7286..8311)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7286..8311)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8321..8665)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8807..8824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8807..8823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8816..8838)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8963..8982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9173..9194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9441..9465
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11061..12209)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12508..13302)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13494..14261
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14954..15585
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggccaa
     4621 tagcagtaac agttctaaga aagatatgga tcgggttaaa ggtccatgga gccccgaaga
     4681 agacgagctt ttacagcagc tcgttcataa acatggacca cgaaattggt ctcttattag
     4741 caaatccata cctggaagat ccggtaaatc ttgcaggtta cgctggtgta atcagttatc
     4801 tccacaagta gagcatcggg cttttactcc cgaagaagat gagaccatta ttcgggccca
     4861 tgctcgattt gggaataaat gggctactat agcccgactt cttaatggac gcaccgataa
     4921 cgccattaag aaccactgga actctacctt gaagaggaag tcctcctctc ttagtgctga
     4981 tgaaggtaac gaactcgccg atcaaatttt tcaaaacgaa cagccgccgt taaagagatc
     5041 cgttagtgcc ggatccgcta taccggtttc gggtttccat ttcagtccct gtagcccatc
     5101 gggttcggac agtgattcga gccttcatgt cacgtcatcg tctcaatctc acttattcaa
     5161 gcctgtctct agagccggcg gtgtgtttcc accgccgtct attgacacat catctccttc
     5221 cgatgatccg ccgacttccc tcagcctttc gcttcccgga gttgacttgg ccgagttttc
     5281 taatcgttcg gctgagtcaa ctcagtcgaa gaatcccttt cagatgcttc ctgttgctat
     5341 gcagattccg ccacctccac caccgccgcc tcaggctgcg gtggttccat ttcaacgtgc
     5401 tcctttaaat caggaagctg caggagtaca gcaagataag gtgtttgtgc cttttagtca
     5461 ggagttgttg ggggtgatgc aggagatgat taaagcggag gtgaggaact atatggtggg
     5521 gattgaacag caacaatttc agcaacaaca gcagcaggaa cgttatcatc aacaacagca
     5581 gcagttgcag caacaaaatc atcaaatgcc aagtggaata gggttaggtt tgtgtatgca
     5641 acaagctagt gatggattca ggaatacagg tgctaatcgc atgggagtaa gcaaaatcga
     5701 tcccggggac ccagctttct tgtacaaagt ggttgataac agcgggttaa ttaacatctt
     5761 ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcagg
     5821 atcctatcca tatgacgttc cagattacgc tgctcagtgc agcgcttaga gctcgaattt
     5881 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5941 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     6001 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     6061 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6121 atctatgtta ctagatcggg aattagcttc atcaacgcaa gacatgcgca cgaccgtctg
     6181 acaggagagg aatttccgac gagcacagaa aggacttgct cttggacgta ggcctatttc
     6241 tcaggcacat gtatcaagtg ttcggacgtg ggttttcgat ggtgtatcag ccgccgccaa
     6301 ctgggagatg aggaggcttt cttggggggc agtcagcagt tcatttcaca agacagagga
     6361 acttgtaagg agatgcactg atttatcttg gcgcaaacca gcaggacgaa ttagtgggaa
     6421 tagcccgcga atatctaagt tatgcctgtc ggcatgagca gaaacttcca attcgaaaca
     6481 gtttggagag gttgtttttg ggcatacctt ttgttagtca gcctctcgat tgctcatcgt
     6541 cattacacag taccgaagtt tgatcgatct agtaacatag atgacaccgc gcgcgataat
     6601 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     6661 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     6721 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     6781 aatcttaaga aactttattg ccaaatgttt gaacgatctg cttcgacgca ctccttcttt
     6841 actccaccat ctcgtcctta ttgaaaacgt gggtagcacc aaaacgaatc aagtcgctgg
     6901 aactgaagtt accaatcacg ctggatgatt tgccagttgg attaatcttg cctttccccg
     6961 catgaataat attgatgaat gcatgcgtga ggggtatttc gattttggca atagctgcaa
     7021 ttgccgcgac atcctccaac gagcataatt cttcagaaaa atagcgatgt tccatgttgt
     7081 cagggcatgc atgatgcacg ttatgaggtg acggtgctag gcagtattcc ctcaaagttt
     7141 catagtcagt atcatattca tcattgcatt cctgcaagag agaattgaga cgcaatccac
     7201 acgctgcggc aaccttccgg cgttcgtggt ctatttgctc ttggacgttg caaacgtaag
     7261 tgttggatcc cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc
     7321 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     7381 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     7441 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     7501 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     7561 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     7621 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     7681 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     7741 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     7801 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     7861 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     7921 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     7981 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     8041 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
     8101 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     8161 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     8221 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     8281 cagacgtcgc ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga
     8341 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     8401 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     8461 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     8521 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     8581 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     8641 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     8701 attcttggag tagacgagag tgtcgtgctc caccatgttg acggatctct aggacgcgtc
     8761 gacgcgtcga cgcgtcgacg cgtcgacgcg tcctagaagc taattcactg gccgtcgttt
     8821 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8881 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8941 tgcgcagcct gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9061 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     9121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9181 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     9241 attttgccga tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     9301 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     9361 tctcactggt gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta
     9421 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     9481 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     9541 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     9601 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     9661 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaaatatca tctccctcgc
     9721 agagatccga attatcagcc ttcttattca tttctcgctt aaccgtgaca ggctgtcgat
     9781 cttgagaact atgccgacat aataggaaat cgctggataa agccgctgag gaagctgagt
     9841 ggcgctattt ctttagaagt gaacgttgac gatatcaact cccctatcca ttgctcaccg
     9901 aatggtacag gtcggggacc cgaagttccg actgtcggcc tgatgcatcc ccggctgatc
     9961 gaccccagat ctggggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga
    10021 gatcccccgg aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag
    10081 gccgagaaca ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg
    10141 aacgagcaga agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg
    10201 aggttgccac ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc
    10261 cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc
    10321 cgcagttccg caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc
    10381 ggcagagatg aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc
    10441 cggcaggcgg tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc
    10501 gaggatgaag atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag
    10561 caataaaccc gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc
    10621 gggctccacg aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg
    10681 tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc
    10741 tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc
    10801 gaacatctct ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc
    10861 ggccgctcca agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt
    10921 tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt
    10981 cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc
    11041 ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg
    11101 tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac
    11161 ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg
    11221 tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg
    11281 cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc
    11341 tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg
    11401 tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc
    11461 gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag
    11521 cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc
    11581 ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg
    11641 ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc
    11701 tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc
    11761 atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg
    11821 atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc
    11881 aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc
    11941 atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc
    12001 gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta
    12061 gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg
    12121 cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg
    12181 tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac
    12241 gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa
    12301 tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg
    12361 gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg
    12421 gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg
    12481 ccggcatcgt tgcgccacat ctaggtacta aaacaattca tccagtaaaa tataatattt
    12541 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    12601 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    12661 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    12721 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    12781 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    12841 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    12901 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    12961 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    13021 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    13081 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    13141 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    13201 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    13261 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    13321 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    13381 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    13441 aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt atgggtgatg
    13501 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
    13561 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
    13621 gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact
    13681 tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt
    13741 ggatgccggg caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta
    13801 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
    13861 gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt
    13921 tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
    13981 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
    14041 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
    14101 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
    14161 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
    14221 atctgaacat aaaacactat caataagttg gagtcattac ccaattatga tagaatttac
    14281 aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg
    14341 cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac
    14401 atacggcgat atcttctata taaaagatat attatcttat cagtattgtc aatatattca
    14461 aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggc
    14521 gcttcataga gtaattctgt aaaggtccaa ttctcgtttt catacctcgg tataatctta
    14581 cctatcacct caaatggttc gctgggttta tcgcaccccc gaacacgagc acggcacccg
    14641 cgaccactat gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga
    14701 atgccccgac ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg
    14761 gcacctggtc gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg
    14821 cgctcgggct gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca
    14881 gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg
    14941 ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg
    15001 cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta
    15061 aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg
    15121 ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca
    15181 gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc
    15241 aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc
    15301 gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga
    15361 aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca
    15421 acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg
    15481 cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag
    15541 acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcggaaa ggcgc
//
