LOCUS       sequence-247456-       16534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16534)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX128
FEATURES             Location/Qualifiers
     source          1..16534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6648..6672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6701..6790
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6824..7076
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7505..7757)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8225..9250)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8225..9250)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9260..9604)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9746..9763)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9746..9762)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9755..9777)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9902..9921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10112..10133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10380..10404
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12000..13148)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13447..14241)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14433..15200
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15893..16524
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatgtc taacgaatgc ggaaacaaag agcatgaatt
     5881 tcaggagatt tctgcctgca gaaacaaagg gttcatgctc tttggtgtta gagtgatgga
     5941 agctggaaat tctattagga aaagtgctag catgaacaac cttgctctat ttgaacagca
     6001 acaacaagat tccaacgtcg ccgccggcta tgcttctgat gacgttgttc atccttctgc
     6061 taggacccgt gaacgtaaac gaggagttcc atggacagag gaagagcaca aactattttt
     6121 attaggattg cagaaagtag ggaaaggcga ttggagagga atttcaagaa actttgtcaa
     6181 gacaaggacc cctactcagg tggccagcca tgctcagaag tactttctcc gccgaaataa
     6241 catcaataac cgccgacgcc gccgctatag tctttttgac atcaccactc atactcatgt
     6301 tcaaagtgtt gctaaattgg aagataagca aatgaatcaa aacaccatag aaaagtttcc
     6361 aatgtcagct tttcgagaga cattcgagca ggcaaaatta tctggtacat gtgaaagctc
     6421 gacggataac ttaatgattg gactgagcaa atctgtgaag cccattaggc caataccatt
     6481 tctccctccg tcatcaaaaa tggcaaatct caatttaaac aagaaatatg tccaagaatc
     6541 ggtcgaaaaa tcgcctccgg cgaggcattc atcggctttc caggcaatgt caagtgggtt
     6601 caatggtaat actagtgggg atagcataat tagtgtggct cccggggacc cagctttctt
     6661 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     6721 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     6781 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     6841 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     6901 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6961 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     7021 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     7081 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     7141 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     7201 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     7261 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     7321 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     7381 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     7441 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     7501 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     7561 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     7621 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     7681 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     7741 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     7801 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     7861 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     7921 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     7981 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     8041 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     8101 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     8161 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     8221 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     8281 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     8341 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     8401 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     8461 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     8521 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     8581 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     8641 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     8701 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     8761 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     8821 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     8881 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     8941 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     9001 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     9061 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     9121 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     9181 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     9241 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     9301 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     9361 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     9421 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     9481 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     9541 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     9601 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     9661 gtcgtgctcc accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc
     9721 gtcgacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc
     9901 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9961 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10021 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10081 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10141 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca
    10201 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
    10261 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
    10321 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    10381 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    10441 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    10501 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    10561 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    10621 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
    10681 tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata
    10741 ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg
    10801 aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc
    10861 gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag
    10921 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
    10981 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
    11041 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
    11101 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
    11161 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
    11221 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
    11281 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
    11341 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
    11401 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
    11461 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
    11521 ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga
    11581 cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat
    11641 gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc
    11701 ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt
    11761 cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat
    11821 ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc
    11881 gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc
    11941 tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    12001 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    12061 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    12121 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    12181 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    12241 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    12301 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    12361 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    12421 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    12481 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    12541 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    12601 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    12661 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    12721 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    12781 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    12841 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    12901 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    12961 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    13021 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    13081 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    13141 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
    13201 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
    13261 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
    13321 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
    13381 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
    13441 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    13501 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    13561 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    13621 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    13681 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    13741 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    13801 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    13861 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    13921 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    13981 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    14041 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    14101 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    14161 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    14221 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    14281 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    14341 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    14401 tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt
    14461 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    14521 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
    14581 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
    14641 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
    14701 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
    14761 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
    14821 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
    14881 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
    14941 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
    15001 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
    15061 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
    15121 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
    15181 aataagttgg agtcattacc caattatgat agaatttaca agctataagg ttattgtcct
    15241 gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga
    15301 taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat
    15361 aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc
    15421 tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta
    15481 aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg
    15541 ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc
    15601 ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga
    15661 agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg
    15721 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
    15781 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga
    15841 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    15901 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    15961 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    16021 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    16081 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    16141 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    16201 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    16261 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    16321 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    16381 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
    16441 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
    16501 agggcaacca gcccggtgag cgtcggaaag gcgc
//
