LOCUS       sequence-247461-       15304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15304)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX130
FEATURES             Location/Qualifiers
     source          1..15304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5418..5442)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5471..5560
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5594..5846
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6275..6527)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6995..8020)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6995..8020)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8030..8374)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8516..8533)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8516..8532)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8525..8547)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8672..8691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8882..8903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9150..9174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10770..11918)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12217..13011)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13203..13970
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14663..15294
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgggaag
     4621 atcaccttgc tgtgagaaat cacatacaaa taaaggagca tggactaaag aagaagatga
     4681 aaggcttata gcttacatta aagttcatgg cgaaggctgt tggaggtcac ttcctaaagc
     4741 tgctggactt ttgaggtgtg gcaaaagttg ccgtctccga tggattaatt acttgaggcc
     4801 tgatctcaaa cgcggtaatt ttactgatga agaagatgaa ctcattatta aactccacag
     4861 cctcctcgga aacaagtggt cgcttatagc aggaagacta ccgggaagaa cagataatga
     4921 gataaagaat tattggaata ctcatataag gaggaaactg ttgagtcgcg gaattgatcc
     4981 tacaacacac aggcctatga acgagcctaa cacacaaaaa gtgacatcaa tttcttttgc
     5041 ttctggtaat tataaagata ttgaagatga aaagatgaac attaaagctg aatttggaac
     5101 aatcaaggaa gatgaaatta gtagcagacc acagtgtcct gatttgaatc ttgagctcag
     5161 gattagccct ccttaccaac aaaaccaaca gaagagggca ttggaacaaa gtagtactgg
     5221 ttcgtggagg attagcccta tatgttttaa atgcaattta gggttaaaga atagtaaaga
     5281 ttgcagttgt aatgacaata gtaatggaaa tggttgcagc agtagcagta atataagtat
     5341 gaatattgct gcttatgact ttctaggatt aaagacaaat ggtttggact acagaacctt
     5401 ggagactaag cccggggacc cagctttctt gtacaaagtg gttgataaca gcgggttaat
     5461 taacatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5521 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag
     5581 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5641 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5701 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5761 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     5821 cgcggtgtca tctatgttac tagatcggga attagcttca tcaacgcaag acatgcgcac
     5881 gaccgtctga caggagagga atttccgacg agcacagaaa ggacttgctc ttggacgtag
     5941 gcctatttct caggcacatg tatcaagtgt tcggacgtgg gttttcgatg gtgtatcagc
     6001 cgccgccaac tgggagatga ggaggctttc ttggggggca gtcagcagtt catttcacaa
     6061 gacagaggaa cttgtaagga gatgcactga tttatcttgg cgcaaaccag caggacgaat
     6121 tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg gcatgagcag aaacttccaa
     6181 ttcgaaacag tttggagagg ttgtttttgg gcataccttt tgttagtcag cctctcgatt
     6241 gctcatcgtc attacacagt accgaagttt gatcgatcta gtaacataga tgacaccgcg
     6301 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     6361 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     6421 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     6481 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttcgacgcac
     6541 tccttcttta ctccaccatc tcgtccttat tgaaaacgtg ggtagcacca aaacgaatca
     6601 agtcgctgga actgaagtta ccaatcacgc tggatgattt gccagttgga ttaatcttgc
     6661 ctttccccgc atgaataata ttgatgaatg catgcgtgag gggtatttcg attttggcaa
     6721 tagctgcaat tgccgcgaca tcctccaacg agcataattc ttcagaaaaa tagcgatgtt
     6781 ccatgttgtc agggcatgca tgatgcacgt tatgaggtga cggtgctagg cagtattccc
     6841 tcaaagtttc atagtcagta tcatattcat cattgcattc ctgcaagaga gaattgagac
     6901 gcaatccaca cgctgcggca accttccggc gttcgtggtc tatttgctct tggacgttgc
     6961 aaacgtaagt gttggatccc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     7021 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7081 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7141 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7201 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7261 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7321 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7381 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7441 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7501 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7561 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7621 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7681 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     7741 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     7801 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     7861 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     7921 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     7981 acttctcgac agacgtcgcg gtgagttcag gctttttcat atcggggtcg tcctctccaa
     8041 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     8101 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     8161 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8221 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     8281 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     8341 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     8401 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga cggatctcta
     8461 ggacgcgtcg acgcgtcgac gcgtcgacgc gtcgacgcgt cctagaagct aattcactgg
     8521 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8581 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8641 cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc ttcctttctc
     8701 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8761 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     8821 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8881 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     8941 ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc
     9001 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     9061 tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat
     9121 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     9181 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     9241 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     9301 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     9361 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat
     9421 ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag
     9481 gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg
     9541 aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc ccctatccat
     9601 tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct gatgcatccc
     9661 cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa ctgtaggttc
     9721 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     9781 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     9841 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     9901 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     9961 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
    10021 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
    10081 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
    10141 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
    10201 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
    10261 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
    10321 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
    10381 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
    10441 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
    10501 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
    10561 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
    10621 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
    10681 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
    10741 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
    10801 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
    10861 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
    10921 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
    10981 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
    11041 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
    11101 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
    11161 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
    11221 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
    11281 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
    11341 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
    11401 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
    11461 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
    11521 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
    11581 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
    11641 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
    11701 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
    11761 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
    11821 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
    11881 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
    11941 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
    12001 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
    12061 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
    12121 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
    12181 tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat
    12241 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
    12301 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
    12361 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
    12421 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
    12481 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
    12541 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
    12601 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
    12661 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
    12721 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
    12781 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
    12841 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
    12901 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
    12961 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
    13021 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
    13081 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
    13141 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta
    13201 tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
    13261 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
    13321 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
    13381 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
    13441 aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta
    13501 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
    13561 tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc
    13621 gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg
    13681 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
    13741 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
    13801 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
    13861 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
    13921 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caattatgat
    13981 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
    14041 tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct tgccccctga
    14101 aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc agtattgtca
    14161 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
    14221 aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc atacctcggt
    14281 ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg aacacgagca
    14341 cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc cccgccatga
    14401 agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg ccgccgccct
    14461 cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc
    14521 cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg gcaatggcaa
    14581 ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
    14641 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
    14701 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
    14761 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
    14821 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
    14881 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
    14941 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
    15001 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
    15061 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
    15121 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
    15181 caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
    15241 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcggaaag
    15301 gcgc
//
