LOCUS       sequence-247464-       15430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15430)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX131
FEATURES             Location/Qualifiers
     source          1..15430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5544..5568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5597..5686
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5720..5972
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6401..6653)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7121..8146)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7121..8146)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8156..8500)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8642..8659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8642..8658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8651..8673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8798..8817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9008..9029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9276..9300
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10896..12044)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12343..13137)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13329..14096
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14789..15420
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggtcat
     4621 ggcccttcct aatcttggct ccttctccaa cccaaccgcc accgcttccg ccgccgcaac
     4681 tgcgtcggcc tccaacccgt tatcgccttc cgatgacccg tcaaagaaaa ttcgaaaacc
     4741 ctacaccatt accaagtctc gcgaaagctg gaccgaaccc gagcacgata agttccttga
     4801 agctcttcaa ctgtttgacc gggactggaa aaagattgaa gcgtttgttg gatcaaaaac
     4861 tgttattcag atacgtagcc atgctcaaaa atattttctg aaggtccaga agagtggaac
     4921 caatgaacat ctacctcctc ctcggccaaa aagaaaagct gctcatccct accctcagaa
     4981 agcctcaaaa agtgctccag ctctctcacc gatgactgca tcatttcaag cttcacttcc
     5041 attaacagat cctggatttg taacaaggcc tgatgcttct ttgttaccta gcaatccagt
     5101 tactgttgca tctatgccat catggactga caactctgtg ccaccagtca gtttgtctcg
     5161 agtgaagaat gataacgtga gagaaccagg tcaacctgtg gccaatactc attgttgcag
     5221 tagcaacgaa agcactccag gatcaaaatc aacaggtgaa gtgatggagc ggactcaggg
     5281 accttcattg agagttttgc ctgactttgt tcaagtatac aacttcatcg gcagcgtatt
     5341 cgatcctgct gttaccggac acttgcagaa attgaaaaaa atggatcgca ttgatgttga
     5401 gacggtgttg ttgctgatga gaaacctctc catcaatctt acaagccctg attttgagca
     5461 tcataggcag ctgctttcat cttatgatat ggacatggag aagcagtgca aatcaagtca
     5521 tcaattacaa ccaactcccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     5581 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     5641 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     5701 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     5761 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     5821 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     5881 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     5941 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     6001 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     6061 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     6121 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     6181 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     6241 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     6301 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     6361 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     6421 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     6481 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     6541 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     6601 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     6661 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     6721 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     6781 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     6841 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     6901 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     6961 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     7021 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     7081 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     7141 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7201 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7261 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7321 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     7381 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     7441 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     7501 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     7561 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     7621 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     7681 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     7741 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     7801 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7861 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     7921 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     7981 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     8041 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     8101 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     8161 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8221 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8281 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8341 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8401 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8461 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8521 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     8581 tctctaggac gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt
     8641 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8701 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8761 gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc
     8821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     8941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     9061 tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc
     9121 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     9181 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc
     9241 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     9301 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     9361 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     9421 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     9481 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa
     9541 tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg
     9601 tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg
     9661 ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct
     9721 atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg
     9781 catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt
     9841 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
     9901 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
     9961 cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt
    10021 gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac
    10081 cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac
    10141 caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg
    10201 gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt
    10261 cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga
    10321 aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg
    10381 gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc
    10441 tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt
    10501 ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc
    10561 gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg
    10621 ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg
    10681 gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa
    10741 ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga
    10801 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
    10861 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
    10921 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
    10981 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
    11041 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
    11101 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
    11161 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
    11221 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
    11281 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
    11341 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
    11401 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
    11461 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
    11521 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
    11581 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
    11641 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
    11701 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
    11761 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
    11821 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
    11881 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
    11941 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
    12001 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
    12061 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    12121 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    12181 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    12241 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    12301 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
    12361 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    12421 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    12481 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    12541 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    12601 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    12661 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    12721 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    12781 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    12841 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    12901 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    12961 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    13021 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    13081 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    13141 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    13201 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    13261 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
    13321 caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
    13381 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
    13441 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
    13501 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
    13561 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
    13621 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
    13681 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
    13741 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
    13801 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
    13861 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
    13921 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
    13981 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
    14041 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaat
    14101 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
    14161 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
    14221 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
    14281 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
    14341 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
    14401 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca
    14461 cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg
    14521 ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc
    14581 cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa
    14641 tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa
    14701 tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
    14761 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
    14821 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
    14881 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
    14941 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
    15001 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
    15061 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
    15121 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
    15181 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
    15241 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
    15301 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    15361 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
    15421 ggaaaggcgc
//
