LOCUS       sequence-247484-       15203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15203)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX136
FEATURES             Location/Qualifiers
     source          1..15203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4867..4878)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5317..5341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5370..5459
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5493..5745
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6174..6426)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6894..7919)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6894..7919)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7929..8273)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8415..8432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8415..8431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8424..8446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8571..8590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8781..8802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9049..9073
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10669..11817)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12116..12910)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13102..13869
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14562..15193
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtctcgtcta
     4621 aaacgccaat ggaaaacaat cagcaatcgg cggcgaatgc agcggcggcg gcagcagcag
     4681 cagctgcata tccagcgcag ccaccgtacc accatctcct tcagcaacag caacagcagc
     4741 tccagatgtt ctggacctac cagcgccaag aaatcgaaca ggttaacgat ttcaagaacc
     4801 accaacttcc tcttgcccga atcaagaaga tcatgaaagc tgacgaggat gtccgcatga
     4861 tctccgctga agctcccatt ttattcgcca aagcgtgtga gcttttcatt ctggaactca
     4921 ctattcgttc ctggcttcac gctgaggaaa acaagcgtcg aactttacaa aagaacgaca
     4981 tagctgcagc gattacgcgg actgacattt ttgatttcct tgttgacatt gttccgaggg
     5041 atgagatcaa ggaagagggt ggtgttggtc ttgggcctgc tgggattgtg ggctccacag
     5101 ctagtggtgt gccttactat tacccaccaa tgggccagcc tgctccaccg ggtgtgatga
     5161 tgggcaggcc tgctatgcct ggggttgatc cttcaatgta cgttcagcca ccgccgtcgc
     5221 aggcgtggca gtccgtttgg cagactgctg aggataattc ctatgcaagt ggaggcagca
     5281 gtggacaggg taaccttgat ggtcaaagtc ccggggaccc agctttcttg tacaaagtgg
     5341 ttgataacag cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct
     5401 atccctatga cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg
     5461 ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     5521 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     5581 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     5641 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     5701 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat
     5761 caacgcaaga catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag
     5821 gacttgctct tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg
     5881 ttttcgatgg tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag
     5941 tcagcagttc atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc
     6001 gcaaaccagc aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg
     6061 catgagcaga aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt
     6121 gttagtcagc ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag
     6181 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     6241 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     6301 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     6361 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     6421 acgatctgct tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg
     6481 gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg
     6541 ccagttggat taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg
     6601 ggtatttcga ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct
     6661 tcagaaaaat agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac
     6721 ggtgctaggc agtattccct caaagtttca tagtcagtat catattcatc attgcattcc
     6781 tgcaagagag aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct
     6841 atttgctctt ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc
     6901 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     6961 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     7021 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     7081 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     7141 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     7201 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     7261 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     7321 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     7381 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     7441 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     7501 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     7561 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     7621 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     7681 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     7741 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     7801 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     7861 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     7921 tcggggtcgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     7981 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     8041 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     8101 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     8161 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     8221 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     8281 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     8341 ccatgttgac ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc
     8401 ctagaagcta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8461 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8521 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc
     8581 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8641 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     8701 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8761 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8821 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag
     8881 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     8941 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta
     9001 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     9061 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     9121 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     9181 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     9241 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     9301 gcctgtgatc aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt
     9361 tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg
     9421 ctggataaag ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga
     9481 tatcaactcc cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac
     9541 tgtcggcctg atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta
     9601 aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac
     9661 ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga
     9721 ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc
     9781 aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga
     9841 acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt
     9901 ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca
     9961 tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca
    10021 cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc
    10081 tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg
    10141 ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca
    10201 taccggcgac ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct
    10261 tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt
    10321 cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct
    10381 ttttctcctc gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca
    10441 atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa
    10501 tcacaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc
    10561 cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag
    10621 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
    10681 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
    10741 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
    10801 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
    10861 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
    10921 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
    10981 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
    11041 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
    11101 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
    11161 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
    11221 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
    11281 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
    11341 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
    11401 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
    11461 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
    11521 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
    11581 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
    11641 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa
    11701 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
    11761 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
    11821 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
    11881 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
    11941 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
    12001 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
    12061 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa
    12121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    12181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    12241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    12301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    12361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    12421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    12481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    12541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    12601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    12661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    12721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    12781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    12841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    12901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    12961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    13021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    13081 gattttgaaa ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg
    13141 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
    13201 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
    13261 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
    13321 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
    13381 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
    13441 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc
    13501 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt
    13561 gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg
    13621 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
    13681 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
    13741 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
    13801 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
    13861 gtcattaccc aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
    13921 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
    13981 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagatatat
    14041 tatcttatca gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc
    14101 ttcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt
    14161 ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc
    14221 gcacccccga acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa
    14281 attgccggcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg
    14341 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc
    14401 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc
    14461 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg
    14521 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
    14581 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
    14641 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
    14701 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
    14761 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
    14821 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
    14881 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
    14941 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
    15001 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
    15061 gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg
    15121 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
    15181 cccggtgagc gtcggaaagg cgc
//
