LOCUS       sequence-247488-       15540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15540)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX138
FEATURES             Location/Qualifiers
     source          1..15540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5654..5678)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5707..5796
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5830..6082
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6511..6763)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7231..8256)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7231..8256)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8266..8610)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8752..8769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8752..8768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8761..8783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8908..8927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9118..9139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9386..9410
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11006..12154)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12453..13247)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13439..14206
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14899..15530
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg ggtctcaaat tgaaatgtcc
     4621 tttggtaaaa ggacctgtac gtcaagtccg aaaaaattcc cttttctttt caattgaaaa
     4681 ataccttcga tttgcccctc tctcactccc aatgattgtg ttcttctgat tcttcccaat
     4741 ttcacaaatt tacatctcaa gattctctta tttattttaa tatccttcag attctcccaa
     4801 tttctgaatg tccagaaact gatttttgtc atgaggaaaa aactcgacac tcgcttccct
     4861 gcggcccgga tcaagaagat tatgcaagct gatgaggatg ttggtaagat tgcaatggcc
     4921 gtgcccgttc tagtgtcaaa agccttggag ttattcttac aagacctttg tgatcggaca
     4981 tatgacataa ctcttagaag aggagcaaag actgttaatt cactgcattt aaagcattgt
     5041 gtacaaagct acaacgtgtt tgactttctt cgggaagttg tcagcaaggt tcctgattat
     5101 ggccattctg atgctgcggg tgaaatgcca aaaagaagga aagttgcaat ggaagaacat
     5161 catgacagtg aggatgagtt caaaaggagt aggacggaga cgggtcatgc tagtggtagg
     5221 ggaagaggta ggggtagagg aagaggtcgt ggccgcggac gtgctgatag ggagctttca
     5281 cggcctgata tgcaacttga atcttgcacc tctgttcagc agagtggtct gcagaatcca
     5341 aaccctggaa ctcacacaga caactgcact gaaccgaagg gatcacctaa actggattcc
     5401 agtgcttgtg acagggaaag ttcagttgta acaactccta atctgaaagt cagcgtagat
     5461 ggaagcgttg ataaatcagc tgcaccagat atagcagcct gcaatccatc gccaaggcct
     5521 gccaatgaga aagtggagga aggcccccaa tggtctctcg aaatggagag aatggttatc
     5581 ggccctgccc atatgtcaca actgaattca agtttggcgg aagaggaaga agattatgat
     5641 gaagagcccg gggacccagc tttcttgtac aaagtggttg ataacagcgg gttaattaac
     5701 atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
     5761 gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc ttagagctcg
     5821 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     5881 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     5941 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     6001 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6061 gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc
     6121 gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg acgtaggcct
     6181 atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc
     6241 gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt tcacaagaca
     6301 gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg acgaattagt
     6361 gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac ttccaattcg
     6421 aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc tcgattgctc
     6481 atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac accgcgcgcg
     6541 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     6601 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     6661 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     6721 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg acgcactcct
     6781 tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc
     6841 gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt
     6901 ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc
     6961 tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat
     7021 gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa
     7081 agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa
     7141 tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac
     7201 gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     7261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     7321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     7381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     7441 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     7501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     7561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     7621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     7681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     7741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     7801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     7861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     7921 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     7981 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     8041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     8101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     8161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     8221 ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct ctccaaatga
     8281 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     8341 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     8401 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     8461 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     8521 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     8581 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     8641 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga tctctaggac
     8701 gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt cactggccgt
     8761 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8821 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8881 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8941 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9001 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     9061 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9121 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     9181 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     9241 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     9301 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     9361 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     9421 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     9481 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     9541 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     9601 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     9661 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     9721 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     9781 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     9841 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     9901 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     9961 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
    10021 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
    10081 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
    10141 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
    10201 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
    10261 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
    10321 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
    10381 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
    10441 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
    10501 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
    10561 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
    10621 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
    10681 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
    10741 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
    10801 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
    10861 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
    10921 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    10981 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    11041 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    11101 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    11161 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    11221 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    11281 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    11341 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    11401 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    11461 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    11521 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    11581 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    11641 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    11701 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    11761 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    11821 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    11881 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    11941 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    12001 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    12061 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    12121 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    12181 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    12241 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    12301 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    12361 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    12421 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    12481 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    12541 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    12601 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    12661 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    12721 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    12781 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    12841 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    12901 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12961 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    13021 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    13081 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    13141 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    13201 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    13261 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    13321 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    13381 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    13441 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    13501 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    13561 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    13621 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    13681 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    13741 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    13801 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    13861 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    13921 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    13981 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    14041 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    14101 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    14161 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    14221 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    14281 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    14341 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    14401 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    14461 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    14521 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    14581 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    14641 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    14701 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    14761 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    14821 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    14881 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    14941 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    15001 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    15061 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    15121 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    15181 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    15241 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    15301 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    15361 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    15421 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    15481 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
