LOCUS       sequence-247514-       15944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15944)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX148
FEATURES             Location/Qualifiers
     source          1..15944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6143..6232
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6266..6518
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6947..7199)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7667..8692)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7667..8692)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8702..9046)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9156..9173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9156..9172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9165..9187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9312..9331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9522..9543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9790..9814
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11410..12558)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12857..13651)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13843..14610
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15303..15934
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg ggtattcaag attatggctt
     4621 tttggaagat cttcaagtgg aattcaaaga ttatgattgc acaaatggga tttttctatg
     4681 cttctggttt tacttgaaca aggcctcaac tttaccctcc acaattcttc tgcagaatca
     4741 ttcagatgtc acatcccatg ctcctttcct gttacttgat gagaagagga gacttttact
     4801 ttatccttta ctaacttcgt ggagggaaga tgtgtatgca acttcagaaa gtgaattttc
     4861 tacgaagaaa tgggttcatg ttggatgtga ggtcacaaaa aatcttttgc gcctctacct
     4921 tgacggggag attgtaggcg agaagtcttt gacgtcctta ctaaacaatg attcagtatc
     4981 agacagttct gaaatatcgt ttccttgcat tagtggtaag ggttctcagc tggatggcta
     5041 tgtgtacagt gcagagctct tgccagtagt atcatctatc aaaaatcact atgttaagga
     5101 ccctccagta caactatcta tcgatagttc aagcgcgtat gaaattgaag aagacactga
     5161 tggtgtttgg agtattgttg gtggaaaggc ctcctgccgg agaaattttt ctttagatgt
     5221 aacactgatg gataacttta gtcgccccat aaccgaagaa gtggaggttg ttgcttcgct
     5281 tgtgtacgcc gatgacgaag caccagttga gaagccagtt gatgatgagg cacctcttct
     5341 tacaaattat gatggaattg aatatgcttc ttctgataga ccaagcaaag taattagtgg
     5401 acgtgcttcc tttaagctca agatatctca gctttcatcc aagtgcgata acaggctctt
     5461 ctgcattagg tttgtgattc caaaaatggg taattatcct ttccttgagg tattctccag
     5521 accaatccgg tgtatttctc ggaatcgtaa cactagggcg gcctctctaa tgttgaggaa
     5581 atcatctttg ggaatccatt tactaaatgg ttctcaatct cctatattgg aggatggatc
     5641 ttctgatctg ccctgtattg tacgtgaagc aaaacaaagt ccttcatcaa agcgggtcaa
     5701 attaggacaa gagaaactat gtgcaaattt taaggatggt tttgtgctgc agcaagctaa
     5761 tggtgaatca aaatctcatt cccggatctc tgaggataac tatgcatatc tgaattcttt
     5821 ggtggcaaga ccagtaagcc atggtggggc acaaaatttc tcctctgatt cagagaacag
     5881 tgaaaccaca aactctggta ttgatgactt tccaagtaac agagatccaa tctcagatgt
     5941 ggatgttttt aaatactgcc tcggtgacct gaatgagagg tgtcttcttc tcaaggaaat
     6001 ggcaatgagt gctaaagaag aagaacttgc aacctttgcg gagagagttt ccttattctc
     6061 tggatgttca caccacaggt atcccgggga cccagctttc ctgtacaaag tggttgataa
     6121 cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg gctatcccta
     6181 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     6241 cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     6301 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     6361 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     6421 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     6481 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt catcaacgca
     6541 agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga aaggacttgc
     6601 tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt gggttttcga
     6661 tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg cagtcagcag
     6721 ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt ggcgcaaacc
     6781 agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt cggcatgagc
     6841 agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct tttgttagtc
     6901 agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc tagtaacata
     6961 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     7021 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     7081 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     7141 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     7201 gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg tgggtagcac
     7261 caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat ttgccagttg
     7321 gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg aggggtattt
     7381 cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat tcttcagaaa
     7441 aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt gacggtgcta
     7501 ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat tcctgcaaga
     7561 gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg tctatttgct
     7621 cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat tcctttgccc
     7681 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     7741 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     7801 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     7861 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     7921 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     7981 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     8041 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     8101 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     8161 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     8221 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     8281 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     8341 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     8401 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     8461 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     8521 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     8581 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     8641 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcggggt
     8701 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     8761 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     8821 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     8881 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     8941 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     9001 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     9061 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     9121 gacggatctc taggacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt
     9181 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9241 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9301 aatggcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9421 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9481 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9541 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     9601 ttcggaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
     9661 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
     9721 aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     9781 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     9841 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     9901 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     9961 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
    10021 taacgatgac agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa
    10081 ttatcagcct tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta
    10141 tgccgacata ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc
    10201 tttagaagtg aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg
    10261 tcggggaccc gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc
    10321 tggggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga
    10381 accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat
    10441 tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa
    10501 gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact
    10561 tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc
    10621 cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc
    10681 aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga
    10741 acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt
    10801 agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga
    10861 tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg
    10921 ccggcaacgc ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga
    10981 aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg
    11041 acagcccaat gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt
    11101 cagcgaacgc ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg
    11161 gagctttctt cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa
    11221 gccgtcgaat ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt
    11281 cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc
    11341 cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc
    11401 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
    11461 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
    11521 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
    11581 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
    11641 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
    11701 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
    11761 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
    11821 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
    11881 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
    11941 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
    12001 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
    12061 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
    12121 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
    12181 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
    12241 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
    12301 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
    12361 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
    12421 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
    12481 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
    12541 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
    12601 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
    12661 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
    12721 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
    12781 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
    12841 gcgccacatc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    12901 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    12961 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    13021 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    13081 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    13141 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    13201 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    13261 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    13321 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    13381 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    13441 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    13501 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    13561 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    13621 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    13681 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    13741 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    13801 taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta
    13861 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
    13921 tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca
    13981 gcgctatctc tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc
    14041 tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc
    14101 aacagcccgc attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg
    14161 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
    14221 gacgaacagt ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
    14281 gacagtctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
    14341 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg
    14401 ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag
    14461 cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg
    14521 ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata
    14581 aaacactatc aataagttgg agtcattacc caattatgat agaatttaca agctataagg
    14641 ttattgtcct gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata
    14701 gatatattga taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata
    14761 tcttctatat aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc
    14821 ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag
    14881 taattctgta aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc
    14941 aaatggttcg ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg
    15001 ccaagaatgc ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg
    15061 gccgaagtga agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg
    15121 ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg
    15181 atcgcccatc ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt
    15241 tttggggtga ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg
    15301 ttagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
    15361 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
    15421 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
    15481 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
    15541 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
    15601 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
    15661 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
    15721 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
    15781 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg
    15841 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
    15901 cccagcggcg agggcaacca gcccggtgag cgtcggaaag gcgc
//
