LOCUS       sequence-247548-       15291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15291)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX152
FEATURES             Location/Qualifiers
     source          1..15291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5405..5429)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5458..5547
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5581..5833
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6262..6514)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6982..8007)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6982..8007)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8017..8361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8503..8520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8503..8519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8512..8534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8659..8678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8869..8890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9137..9161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10757..11905)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12204..12998)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13190..13957
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14650..15281
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatatgtcg
     4621 acttcagcag aaggtaacgg agctataatt gatcctcatc aaaggcagca acagcaaaat
     4681 ccttcggggg gcggcggtac aaacggcgct ttaatagtga aaaagccgcc agtaaaagac
     4741 cgtcatagca aagttgacgg tagaggaagg cgtataagga tgccgattgt atgtgcagct
     4801 agggtttttc agctcacccg tgaactaggt cacaagtctg atggccaaac tattgaatgg
     4861 ttgcttcgtc aagccgagcc gtcaattata gctgcaactg gtactggtac aataccaact
     4921 agtttctcta ctgtatccgt ttcagtacga agctcatcgt catcgatatt aacttcatgc
     4981 atttcggctc cgcttgatca taagcagccg ccatcatcac agccgttgat ttctcctgct
     5041 ccgtttatac tcgggaaacg cctccggcct gatgatgaga ccattgaaaa cgggcataag
     5101 gaggatgtgg gacgtgcagc tacggttgtg ggtcccacgt ccggattctg tgctgttcca
     5161 gctcggccag attttggcca agtttggagc tttgctgcgg cgccaccaga aatgatggtg
     5221 ccaacatcag cagcgttaag tttgcagagt tcaagattct ttcagcagca aatgggagag
     5281 gcttcagcag ctagagtggg gaattattta cccatgactc aaggacacct caatttgctg
     5341 gcttctttat caggtccgcc acaaccttct tctggtagaa gagacgacga cggacgcccc
     5401 ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa catcttttac
     5461 ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcc
     5521 tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc gaatttcccc
     5581 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     5641 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     5701 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     5761 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     5821 atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac cgtctgacag
     5881 gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc tatttctcag
     5941 gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc cgccaactgg
     6001 gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac agaggaactt
     6061 gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag tgggaatagc
     6121 ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc gaaacagttt
     6181 ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct catcgtcatt
     6241 acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc gataatttat
     6301 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
     6361 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
     6421 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
     6481 ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc ttctttactc
     6541 caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt cgctggaact
     6601 gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt tccccgcatg
     6661 aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag ctgcaattgc
     6721 cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca tgttgtcagg
     6781 gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca aagtttcata
     6841 gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca atccacacgc
     6901 tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa cgtaagtgtt
     6961 ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt
     7021 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     7081 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     7141 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     7201 aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc
     7261 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     7321 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     7381 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
     7441 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     7501 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     7561 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     7621 ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc
     7681 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
     7741 ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc
     7801 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     7861 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     7921 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     7981 cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg aaatgaactt
     8041 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     8101 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     8161 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     8221 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     8281 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     8341 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     8401 ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga cgcgtcgacg
     8461 cgtcgacgcg tcgacgcgtc gacgcgtcct agaagctaat tcactggccg tcgttttaca
     8521 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8581 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8641 cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8701 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8761 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     8821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8881 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     8941 tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     9001 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     9061 actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     9121 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     9181 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     9241 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     9301 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     9361 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag
     9421 atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct gtcgatcttg
     9481 agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg
     9541 ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc tcaccgaatg
     9601 gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg ctgatcgacc
     9661 ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc
     9721 ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg
     9781 agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg
     9841 agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt
     9901 tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct
     9961 gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca
    10021 gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca
    10081 gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc
    10141 aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg
    10201 atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat
    10261 aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc
    10321 tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg
    10381 aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc
    10441 aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac
    10501 atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc
    10561 gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc
    10621 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
    10681 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
    10741 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
    10801 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
    10861 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
    10921 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
    10981 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
    11041 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
    11101 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
    11161 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
    11221 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
    11281 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
    11341 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
    11401 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
    11461 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
    11521 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
    11581 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
    11641 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
    11701 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
    11761 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
    11821 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
    11881 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
    11941 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
    12001 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
    12061 tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct
    12121 cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg
    12181 catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata atattttatt
    12241 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    12301 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    12361 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    12421 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    12481 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    12541 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    12601 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    12661 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    12721 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    12781 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    12841 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    12901 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    12961 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    13021 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    13081 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    13141 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc
    13201 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
    13261 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
    13321 gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca
    13381 ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat
    13441 gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa
    13501 actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc
    13561 ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta
    13621 cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc
    13681 gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga
    13741 tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata
    13801 tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg
    13861 gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct
    13921 gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga atttacaagc
    13981 tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat gctttgccca
    14041 ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat ccttacatac
    14101 ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc
    14161 aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatggcgctt
    14221 catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata atcttaccta
    14281 tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg cacccgcgac
    14341 cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc
    14401 cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac
    14461 ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct
    14521 cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc
    14581 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
    14641 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
    14701 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
    14761 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
    14821 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
    14881 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
    14941 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
    15001 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
    15061 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
    15121 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
    15181 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
    15241 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg c
//
