LOCUS       sequence-247551-       15124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15124)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX155
FEATURES             Location/Qualifiers
     source          1..15124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5238..5262)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5291..5380
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5414..5666
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6095..6347)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6815..7840)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6815..7840)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7850..8194)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8336..8353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8336..8352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8345..8367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8492..8511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8702..8723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8970..8994
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10590..11738)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12037..12831)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13023..13790
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14483..15114
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcatc
     4621 atcagagatt gtggattccg gtcgattcgc cgccgccgcc ggtggtcaga aatcacattt
     4681 ctcacagaca tgtaatttgt tgagccaata cttgaaagag aagaaaggtt cctttggaga
     4741 tctcagcctt ggtatccacc gcgccggcac tactactatg gatttgttgc caatgattga
     4801 gaaatccggt gagtcaaacc ctcagaaatc aatggatctg tttcctcaaa ctgaagcaaa
     4861 atctgaaccg gagaaagcac agatgacgat attctatggc ggtcaagtta ttgtgtttaa
     4921 tgattttcca gcagataaag ctgaggaaat catgcttatg gctagttgta ccaaaggaga
     4981 caacaacagt actactcaga ttcaaaaaac agctgaatct gctttagatt tggtatacca
     5041 gcctattatt tctggagatt taccaattgc gagacgagct tcacttacta ggtttttaga
     5101 gaaaagaaaa gataggctga ctgcaaaagc accgtaccaa ttaagcaacc caaataaaca
     5161 agcagcagtt tctgaaaaca aggcgtggct tggattgggt gctcaatttc cagtgaaagc
     5221 tgagcaattc cccggggacc cagctttctt gtacaaagtg gttgataaca gcgggttaat
     5281 taacatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5341 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag
     5401 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5461 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5521 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5581 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     5641 cgcggtgtca tctatgttac tagatcggga attagcttca tcaacgcaag acatgcgcac
     5701 gaccgtctga caggagagga atttccgacg agcacagaaa ggacttgctc ttggacgtag
     5761 gcctatttct caggcacatg tatcaagtgt tcggacgtgg gttttcgatg gtgtatcagc
     5821 cgccgccaac tgggagatga ggaggctttc ttggggggca gtcagcagtt catttcacaa
     5881 gacagaggaa cttgtaagga gatgcactga tttatcttgg cgcaaaccag caggacgaat
     5941 tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg gcatgagcag aaacttccaa
     6001 ttcgaaacag tttggagagg ttgtttttgg gcataccttt tgttagtcag cctctcgatt
     6061 gctcatcgtc attacacagt accgaagttt gatcgatcta gtaacataga tgacaccgcg
     6121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     6181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     6241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     6301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttcgacgcac
     6361 tccttcttta ctccaccatc tcgtccttat tgaaaacgtg ggtagcacca aaacgaatca
     6421 agtcgctgga actgaagtta ccaatcacgc tggatgattt gccagttgga ttaatcttgc
     6481 ctttccccgc atgaataata ttgatgaatg catgcgtgag gggtatttcg attttggcaa
     6541 tagctgcaat tgccgcgaca tcctccaacg agcataattc ttcagaaaaa tagcgatgtt
     6601 ccatgttgtc agggcatgca tgatgcacgt tatgaggtga cggtgctagg cagtattccc
     6661 tcaaagtttc atagtcagta tcatattcat cattgcattc ctgcaagaga gaattgagac
     6721 gcaatccaca cgctgcggca accttccggc gttcgtggtc tatttgctct tggacgttgc
     6781 aaacgtaagt gttggatccc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     6841 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     6901 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     6961 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7021 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7081 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7141 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7201 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7261 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7321 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7381 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7441 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7501 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     7561 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     7621 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     7681 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     7741 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     7801 acttctcgac agacgtcgcg gtgagttcag gctttttcat atcggggtcg tcctctccaa
     7861 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     7921 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     7981 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8041 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     8101 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     8161 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     8221 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga cggatctcta
     8281 ggacgcgtcg acgcgtcgac gcgtcgacgc gtcgacgcgt cctagaagct aattcactgg
     8341 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8401 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8461 cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc ttcctttctc
     8521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     8641 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8701 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     8761 ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc
     8821 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     8881 tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat
     8941 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     9001 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     9061 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     9121 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     9181 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat
     9241 ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag
     9301 gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg
     9361 aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc ccctatccat
     9421 tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct gatgcatccc
     9481 cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa ctgtaggttc
     9541 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     9601 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     9661 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     9721 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     9781 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
     9841 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
     9901 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
     9961 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
    10021 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
    10081 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
    10141 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
    10201 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
    10261 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
    10321 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
    10381 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
    10441 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
    10501 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
    10561 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
    10621 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
    10681 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
    10741 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
    10801 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
    10861 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
    10921 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
    10981 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
    11041 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
    11101 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
    11161 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
    11221 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
    11281 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
    11341 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
    11401 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
    11461 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
    11521 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
    11581 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
    11641 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
    11701 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
    11761 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
    11821 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
    11881 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
    11941 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
    12001 tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat
    12061 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
    12121 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
    12181 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
    12241 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
    12301 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
    12361 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
    12421 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
    12481 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
    12541 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
    12601 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
    12661 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
    12721 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
    12781 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
    12841 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
    12901 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
    12961 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta
    13021 tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
    13081 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
    13141 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
    13201 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
    13261 aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta
    13321 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
    13381 tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc
    13441 gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg
    13501 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
    13561 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
    13621 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
    13681 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
    13741 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caattatgat
    13801 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
    13861 tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct tgccccctga
    13921 aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc agtattgtca
    13981 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
    14041 aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc atacctcggt
    14101 ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg aacacgagca
    14161 cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc cccgccatga
    14221 agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg ccgccgccct
    14281 cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc
    14341 cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg gcaatggcaa
    14401 ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
    14461 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
    14521 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
    14581 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
    14641 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
    14701 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
    14761 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
    14821 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
    14881 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
    14941 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
    15001 caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
    15061 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcggaaag
    15121 gcgc
//
