LOCUS       sequence-247569-       16423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16423)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX162
FEATURES             Location/Qualifiers
     source          1..16423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6537..6561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6590..6679
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6713..6965
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7394..7646)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8114..9139)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8114..9139)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9149..9493)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9635..9652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9635..9651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9644..9666)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9791..9810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10001..10022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10269..10293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11889..13037)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13336..14130)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14322..15089
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15782..16413
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatggc gatattgaaa ctcgcgggac tcctaggctc
     5881 caggaatcat ttgttgcata agagattgcc tggagagggt gtcagagatt ccatctggca
     5941 gcatgccatc aacactcttg cagcaggaaa attgacaggt cgagtgtttg ctccctactc
     6001 agtatttaaa ggcaaggcgg cactttctgc tgaacctaga ctgcccacat ttagcaaact
     6061 ggattctgga ggtgttaaac tcaatcgccg tggtgtcatt atgttgacat tctggccatc
     6121 tgttggtgag cgcaaatatg actgggaaaa gaggcagttg tttgctttat cagcaacaga
     6181 ggttggatca ttgataagta tcgggaccag agatacttgt gaattcttcc atgatccttc
     6241 aatgctatca agcaatgctg gtcaagtaag aaagagcttg tcatttaagc caaatgctga
     6301 tggcagtggt tatttcgttt cattgagtgt ggtcaacaac aatctcaaga ccaatgatcg
     6361 gtttactgtt ccggttacta ctgctgagtt tgctgttatg cggacagctt tcagttttgc
     6421 gctgcctcat atcatgggat gggatcgctt cattaatcaa cctttggaaa gcatctctca
     6481 aagctcctca aaggtggttc cacaacttat ggagacggag tgggatagac ccggggaccc
     6541 agctttcttg tacaaagtgg ttgataacag cgggttaatt aacatctttt acccatacga
     6601 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     6661 tgacgttcca gattacgctg ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc
     6721 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     6781 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     6841 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     6901 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     6961 agatcgggaa ttagcttcat caacgcaaga catgcgcacg accgtctgac aggagaggaa
     7021 tttccgacga gcacagaaag gacttgctct tggacgtagg cctatttctc aggcacatgt
     7081 atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc gccgccaact gggagatgag
     7141 gaggctttct tggggggcag tcagcagttc atttcacaag acagaggaac ttgtaaggag
     7201 atgcactgat ttatcttggc gcaaaccagc aggacgaatt agtgggaata gcccgcgaat
     7261 atctaagtta tgcctgtcgg catgagcaga aacttccaat tcgaaacagt ttggagaggt
     7321 tgtttttggg catacctttt gttagtcagc ctctcgattg ctcatcgtca ttacacagta
     7381 ccgaagtttg atcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     7441 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     7501 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     7561 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     7621 ctttattgcc aaatgtttga acgatctgct tcgacgcact ccttctttac tccaccatct
     7681 cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac
     7741 caatcacgct ggatgatttg ccagttggat taatcttgcc tttccccgca tgaataatat
     7801 tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat agctgcaatt gccgcgacat
     7861 cctccaacga gcataattct tcagaaaaat agcgatgttc catgttgtca gggcatgcat
     7921 gatgcacgtt atgaggtgac ggtgctaggc agtattccct caaagtttca tagtcagtat
     7981 catattcatc attgcattcc tgcaagagag aattgagacg caatccacac gctgcggcaa
     8041 ccttccggcg ttcgtggtct atttgctctt ggacgttgca aacgtaagtg ttggatcccg
     8101 gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     8161 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     8221 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     8281 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     8341 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     8401 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     8461 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     8521 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     8581 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     8641 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     8701 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     8761 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt
     8821 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     8881 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     8941 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     9001 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     9061 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     9121 tgagttcagg ctttttcata tcggggtcgt cctctccaaa tgaaatgaac ttccttatat
     9181 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     9241 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     9301 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     9361 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     9421 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     9481 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     9541 gacgagagtg tcgtgctcca ccatgttgac ggatctctag gacgcgtcga cgcgtcgacg
     9601 cgtcgacgcg tcgacgcgtc ctagaagcta attcactggc cgtcgtttta caacgtcgtg
     9661 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9721 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     9781 atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9901 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10021 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
    10081 tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
    10141 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
    10201 aaagaaaaac caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    10261 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    10321 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    10381 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    10441 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    10501 aacgatgaca gagcgttgct gcctgtgatc aaatatcatc tccctcgcag agatccgaat
    10561 tatcagcctt cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat
    10621 gccgacataa taggaaatcg ctggataaag ccgctgagga agctgagtgg cgctatttct
    10681 ttagaagtga acgttgacga tatcaactcc cctatccatt gctcaccgaa tggtacaggt
    10741 cggggacccg aagttccgac tgtcggcctg atgcatcccc ggctgatcga ccccagatct
    10801 ggggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
    10861 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
    10921 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
    10981 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
    11041 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
    11101 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
    11161 aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa
    11221 cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta
    11281 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
    11341 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
    11401 cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa
    11461 aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga
    11521 cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc
    11581 agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg
    11641 agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag
    11701 ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc
    11761 gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc
    11821 gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc
    11881 acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg
    11941 ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg
    12001 gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg
    12061 tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc
    12121 catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg
    12181 acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac
    12241 accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc
    12301 gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg
    12361 caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc
    12421 aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg
    12481 atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt
    12541 ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc
    12601 gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc
    12661 gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg
    12721 gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc
    12781 aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc
    12841 agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc
    12901 atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg
    12961 gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg
    13021 tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg
    13081 tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg
    13141 gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc
    13201 ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc ctcggcctga
    13261 gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg
    13321 cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
    13381 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
    13441 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
    13501 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
    13561 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
    13621 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    13681 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    13741 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    13801 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    13861 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    13921 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    13981 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    14041 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    14101 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    14161 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    14221 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    14281 aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct gccaacttac
    14341 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
    14401 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
    14461 cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta caccgcttct
    14521 caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg atgccgggca
    14581 acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc
    14641 cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg
    14701 acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg
    14761 acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg
    14821 ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc
    14881 cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc
    14941 gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc
    15001 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
    15061 aacactatca ataagttgga gtcattaccc aattatgata gaatttacaa gctataaggt
    15121 tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc cattctatag
    15181 atatattgat aagcgcgctg cctatgcctt gccccctgaa atccttacat acggcgatat
    15241 cttctatata aaagatatat tatcttatca gtattgtcaa tatattcaag gcaatctgcc
    15301 tcctcatcct cttcatcctc ttcgtcttgg tagcttttta aatatggcgc ttcatagagt
    15361 aattctgtaa aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca
    15421 aatggttcgc tgggtttatc gcacccccga acacgagcac ggcacccgcg accactatgc
    15481 caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat gccccgacgg
    15541 ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc acctggtcgc
    15601 tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga
    15661 tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc gctgccattt
    15721 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
    15781 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
    15841 cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa
    15901 aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg
    15961 cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc
    16021 tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac
    16081 cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag
    16141 gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc
    16201 ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct
    16261 catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt
    16321 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
    16381 ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgc
//
