LOCUS       sequence-24757-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Goldstone RM, Moreland NJ, Bashiri G, Baker EN, Shaun Lott J
  TITLE     A new Gateway vector and expression protocol for fast and efficient 
            recombinant protein expression in Mycobacterium smegmatis.
  JOURNAL   Protein Expr Purif. 2008 Jan . 57(1):81-7.
  PUBMED    17949993
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2008 Jan . 57(1):81-7."
COMMENT     Sequence Label: pDESTsmg
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    90..214
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(239..244,245..262,263..269)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(389..408)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tggcgtnatt ccctctgaat attttgttta ctttaagaag gagatatacc atgtcgcatc
       61 accatcacca tcacctcgaa tcaccatcaa caagtttgta caaaaaagct gaacgagaaa
      121 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      181 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
      241 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
      301 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      361 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      421 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      481 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      541 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      601 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      661 ccggncgtcc cnacccatat attcgcaaga tgtggcgtgn tacngtnnaa acctggccnt
      721 tttccctaaa gggttanngg agatatgttt tccgtctcag ccattccngg gtgagtttng
      781 cagttngatt aacnnggcca atnggnccac tctcnccccg ttnccanggc aaatgtnna
//
