LOCUS       sequence-247570-       16466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16466)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX163
FEATURES             Location/Qualifiers
     source          1..16466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6580..6604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6633..6722
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6756..7008
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7437..7689)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8157..9182)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8157..9182)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9192..9536)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9678..9695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9678..9694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9687..9709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9834..9853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10044..10065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10312..10336
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11932..13080)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13379..14173)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14365..15132
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15825..16456
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtccttt ctatggcgat attgaaactt gctggactcc
     5881 tacgccccag gaatcagttg ttgcataaga gattgcctgg agagggtgtc agagattcca
     5941 tctggcagca tgccatcaac actcttgcag gtttttctac tgttagacag agaattgtgg
     6001 ctgatgcagg aaaattgaca ggtcgagtgt ttgctcccta ctcagtattt aaaggcaagg
     6061 cggcactttc tgctgaacct agactgccca cgtttagcaa actggattct ggaggtgtta
     6121 aactcaatcg ccgtggtgtc attatgttga cattctggcc atctgttggc gagcgcaaat
     6181 atgactggga aaagaggcag ttgtttgctt tatcagcaac agaggttgga tcattgataa
     6241 gtatcgggac cagagattct tgtgaattct ttcatgatcc ttcaatgcta tcaagcaatg
     6301 ctggtcaagt aagaaagagc ttgtctttta agccaaatgc tgatggcact ggttatttca
     6361 tttcattgag tgtggtcaac aataatctca aaaacaataa tcggtttact gttcctgtta
     6421 ctactgctga gtttgctgtt atgcggacag ctttcagttt tgcgctgcct catatcatgg
     6481 gatgggatcg cttcactaat cagccttcag aaagcatctc tcaaaccccc tcaaaggttg
     6541 ttccacaact tatggagacg gagtgggata gacccgggga cccagctttc ttgtacaaag
     6601 tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg
     6661 gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg
     6721 ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     6781 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     6841 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     6901 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     6961 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt
     7021 catcaacgca agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga
     7081 aaggacttgc tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt
     7141 gggttttcga tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg
     7201 cagtcagcag ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt
     7261 ggcgcaaacc agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt
     7321 cggcatgagc agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct
     7381 tttgttagtc agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc
     7441 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     7501 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     7561 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     7621 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     7681 tgaacgatct gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg
     7741 tgggtagcac caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat
     7801 ttgccagttg gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg
     7861 aggggtattt cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat
     7921 tcttcagaaa aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt
     7981 gacggtgcta ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat
     8041 tcctgcaaga gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg
     8101 tctatttgct cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat
     8161 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     8221 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     8281 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     8341 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     8401 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     8461 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     8521 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     8581 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     8641 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     8701 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     8761 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     8821 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     8881 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     8941 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     9001 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     9061 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     9121 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     9181 atatcggggt cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     9241 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     9301 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     9361 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     9421 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     9481 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     9541 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     9601 ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg acgcgtcgac gcgtcgacgc
     9661 gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt
     9841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9961 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10081 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
    10141 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    10201 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca
    10261 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
    10321 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
    10381 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
    10441 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
    10501 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
    10561 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
    10621 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa
    10681 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga
    10741 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc
    10801 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca
    10861 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
    10921 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
    10981 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
    11041 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
    11101 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
    11161 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
    11221 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
    11281 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
    11341 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
    11401 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
    11461 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
    11521 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
    11581 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
    11641 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
    11701 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct
    11761 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
    11821 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
    11881 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
    11941 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
    12001 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
    12061 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
    12121 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
    12181 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
    12241 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
    12301 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
    12361 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
    12421 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
    12481 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
    12541 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
    12601 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
    12661 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
    12721 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
    12781 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
    12841 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
    12901 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
    12961 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
    13021 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
    13081 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
    13141 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
    13201 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
    13261 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat
    13321 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact
    13381 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    13441 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    13501 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    13561 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    13621 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    13681 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    13741 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    13801 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    13861 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    13921 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    13981 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    14041 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    14101 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    14161 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    14221 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    14281 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    14341 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg
    14401 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
    14461 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    14521 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    14581 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    14641 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    14701 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    14761 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    14821 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    14881 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    14941 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    15001 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    15061 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    15121 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca
    15181 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg
    15241 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata
    15301 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
    15361 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
    15421 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
    15481 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta
    15541 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg
    15601 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc
    15661 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact
    15721 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc
    15781 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    15841 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    15901 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    15961 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    16021 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    16081 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    16141 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    16201 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    16261 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    16321 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
    16381 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
    16441 cagcccggtg agcgtcggaa aggcgc
//
