LOCUS       sequence-247605-       16165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16165)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX184
FEATURES             Location/Qualifiers
     source          1..16165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6279..6303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6332..6421
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6455..6707
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7136..7388)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7856..8881)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7856..8881)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8891..9235)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9377..9394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9377..9393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9386..9408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9533..9552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9743..9764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10011..10035
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11631..12779)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13078..13872)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14064..14831
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15524..16155
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgaatca
     4621 ttcaattcct gattttgata tggatgatga ctacactatt cctacttctt ctggtctaac
     4681 cagacctaaa aagtctgcaa tggcggaaga ggatatcatg gaactattgt ggcataatgg
     4741 tcaagtggtt atgcagagcc aaaatcaaag atctctgaag aaatctcaca ttagcaacga
     4801 cggtggtagc ggtgatgcgc ttattccctc cgaacaagct gtcagtaggg agatccgaca
     4861 tgtagaggaa acaactacac cgcagcagct gtttatgcag gaggacgaga tggcttcatg
     4921 gcttcactac ccacttgatg actcctcctc cttcgaacgt gatctttacg ccaatctcct
     4981 ttattccaca ccgagcgcaa ccgttacaac cgctgcgccg cctcgagaaa tccgtacgcc
     5041 cacggtggag atccgtccac ctccgccgca accgtcccca gcagcgccga ttgcagtggc
     5101 tcctcgaccg cctatacctc ctgcaagacg tcccggcaca gaaagctcac atcggttcca
     5161 gaacttcgga cacttctcgc gattgcctag tcgaacaagg tcagaacttg gtccgtcaaa
     5221 ttcgagcaag tcacctagag aatcaacggt cgtggactca aacgaaactc caatttcagg
     5281 gcctgaatct agagtttcac acgtagcgga taatgtagta ccggttcccg gcggaaatgt
     5341 agcatgtgga gctgtaaatg tcaccggaac tacgactgcg tcaaccgcaa ttagggaacc
     5401 ggcgacgaca tgtgagcttt cggtgacgtc atctcccggc tccggaaaca gtataaacgc
     5461 aagcgctgaa ctaccgccgt cgcaaacggt ggcgttggcg acaccgacgg ctgcggcatc
     5521 gaatgatcgg aagcgcaaag gaatagaaac ggacgatagg gatggtcaga atgaggacgc
     5581 tgaatttgag tctggtgata caaagaagca tgcatgtggc tcaacttcta caaaacgatc
     5641 tcgtgctgca gaggtccata atctttcgga aaggagacgc cgagacagaa taaatgagaa
     5701 gatgagggct ctgcaggaac tgataccacg ctgtaacaag acagacaaag cttcaatgct
     5761 ggatgaggca attgagtact tgaaatcact gcaattgcaa gtgcagatga tgtccatggg
     5821 atgcggcatg gtcccgatga tgtatcctgg aatgcagcaa tatatgccag ctatgggaat
     5881 gggcatggtg ggaatgggta tggagattgg catgaacagg ccaatggtgc catatccacc
     5941 tctattacca ggtgcagcga tgcagaatgc agctgcagca gcacaaatgg gtcctagatt
     6001 tcctatggcg ccgtttcatt tgccaccagt tccagtacca gatccttcca ggatgcaagc
     6061 ctcaagtcag caagatctaa tgctaaatcc acttgtagca cgtaatccca accagccaag
     6121 acttccgaat tttaatgatc catatcaaca gttttttggt cttcaccagg cacaagtgca
     6181 attaccgcag aatcaggcag tagaacagca aggttataat aaatccggca gcagcaaaga
     6241 agttggaaat ccagggaatc ctcaatctgg tcccggggac ccagctttct tgtacaaagt
     6301 ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg
     6361 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
     6421 tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     6481 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6541 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6601 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6661 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattagcttc
     6721 atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac gagcacagaa
     6781 aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg
     6841 ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt cttggggggc
     6901 agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg atttatcttg
     6961 gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt tatgcctgtc
     7021 ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg ggcatacctt
     7081 ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt tgatcgatct
     7141 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     7201 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     7261 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     7321 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     7381 gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta ttgaaaacgt
     7441 gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg ctggatgatt
     7501 tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat gcatgcgtga
     7561 ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac gagcataatt
     7621 cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg ttatgaggtg
     7681 acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca tcattgcatt
     7741 cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg cgttcgtggt
     7801 ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat ctactctatt
     7861 cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
     7921 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
     7981 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
     8041 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
     8101 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
     8161 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
     8221 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
     8281 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
     8341 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
     8401 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
     8461 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
     8521 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
     8581 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
     8641 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
     8701 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
     8761 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
     8821 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
     8881 tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     8941 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     9001 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     9061 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     9121 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     9181 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     9241 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     9301 caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg cgtcgacgcg
     9361 tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9421 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9481 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     9541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9661 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9781 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     9841 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     9901 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
     9961 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    10021 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    10081 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    10141 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    10201 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    10261 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
    10321 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
    10381 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
    10441 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
    10501 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
    10561 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
    10621 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
    10681 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
    10741 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
    10801 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
    10861 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
    10921 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
    10981 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
    11041 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
    11101 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
    11161 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
    11221 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
    11281 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
    11341 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
    11401 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
    11461 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
    11521 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
    11581 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
    11641 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    11701 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    11761 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    11821 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    11881 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    11941 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    12001 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    12061 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    12121 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    12181 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    12241 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    12301 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    12361 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    12421 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    12481 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    12541 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    12601 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    12661 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    12721 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    12781 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    12841 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    12901 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    12961 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    13021 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    13081 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    13141 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    13201 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    13261 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    13321 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    13381 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    13441 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    13501 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    13561 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    13621 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    13681 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    13741 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    13801 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    13861 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    13921 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    13981 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    14041 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    14101 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
    14161 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    14221 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    14281 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    14341 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    14401 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    14461 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    14521 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    14581 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    14641 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    14701 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    14761 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    14821 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    14881 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    14941 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    15001 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    15061 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    15121 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    15181 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    15241 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    15301 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    15361 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    15421 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    15481 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    15541 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    15601 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    15661 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    15721 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    15781 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    15841 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    15901 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    15961 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    16021 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    16081 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    16141 agcccggtga gcgtcggaaa ggcgc
//
